TY - JOUR
T1 - Characterization of five fresh water microalgae with potential for biodiesel production
AU - Valdez-Ojeda, Ruby
AU - González-Muñoz, Muriel
AU - Us-Vázquez, Roberto
AU - Narváez-Zapata, José
AU - Chavarria-Hernandez, Juan Carlos
AU - López-Adrián, Silvia
AU - Barahona-Pérez, Felipe
AU - Toledano-Thompson, Tanit
AU - Garduño-Solórzano, Gloria
AU - Escobedo-Gracia Medrano, Rosa María
N1 - Publisher Copyright:
© 2014 Elsevier B.V.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Microalgae biodiesel feedstocks have been investigated by numerous research groups to overcome dependence on fossil fuels. This study describes a detailed characterization of five freshwater microalgae strains of the family Scenedesmaceae, based on cell wall ultrastructure, ITS-2 sequence and secondary structure analysis, as well as the estimation of biodiesel properties from fatty acids produced before and after N-limitation. Characterization permitted the reclassification of SCRE-1 and SCRE-2 strains into the subgenus Scenedesmus; DSRE-1 and DSRE-2 strains into the subgenus Desmodesmus; and CORE-1 strain into the genus Coelastrum. Transesterification of fatty acids of the five strains was performed before and after N-limitation, and seven important biodiesel quality parameters were predicted by applying selected correlations. These parameters were then compared to the corresponding specifications in the American and European biodiesel standards. N-limitation promoted higher yields of biomass (up to 3.5 times) and lipids (up to 4.1 times) in all strains under study. Moreover, it was found that SCRE-2 was the only biodiesel that met all estimated parameters of the more stringent European standard before N-limitation. This was also true for DSRE-1 and DSRE-2 biodiesels after limitation, with the exception of their oxidative stability parameter, whose values met the limit of the American but not the European standard.
AB - Microalgae biodiesel feedstocks have been investigated by numerous research groups to overcome dependence on fossil fuels. This study describes a detailed characterization of five freshwater microalgae strains of the family Scenedesmaceae, based on cell wall ultrastructure, ITS-2 sequence and secondary structure analysis, as well as the estimation of biodiesel properties from fatty acids produced before and after N-limitation. Characterization permitted the reclassification of SCRE-1 and SCRE-2 strains into the subgenus Scenedesmus; DSRE-1 and DSRE-2 strains into the subgenus Desmodesmus; and CORE-1 strain into the genus Coelastrum. Transesterification of fatty acids of the five strains was performed before and after N-limitation, and seven important biodiesel quality parameters were predicted by applying selected correlations. These parameters were then compared to the corresponding specifications in the American and European biodiesel standards. N-limitation promoted higher yields of biomass (up to 3.5 times) and lipids (up to 4.1 times) in all strains under study. Moreover, it was found that SCRE-2 was the only biodiesel that met all estimated parameters of the more stringent European standard before N-limitation. This was also true for DSRE-1 and DSRE-2 biodiesels after limitation, with the exception of their oxidative stability parameter, whose values met the limit of the American but not the European standard.
KW - Biodiesel properties
KW - CBC analysis
KW - Desmodesmus
KW - Fatty acids
KW - ITS-2
KW - Scenedesmus
UR - http://www.scopus.com/inward/record.url?scp=84921352153&partnerID=8YFLogxK
U2 - 10.1016/j.algal.2014.11.009
DO - 10.1016/j.algal.2014.11.009
M3 - Artículo
SN - 2211-9264
VL - 7
SP - 33
EP - 44
JO - Algal Research
JF - Algal Research
ER -