TY - JOUR
T1 - Changes in the functionality of plasma membrane of rhizopus stolonifer by addition of chitosan
AU - Hernández-Lauzardo, Ana N.
AU - Vega-Pérez, Josam
AU - Velázquez-del Valle, Miguel G.
AU - Sánchez, Norma S.
AU - Peña, Antonio
AU - Guerra-Sánchez, Guadalupe
PY - 2011/8
Y1 - 2011/8
N2 - The effect of chitosan (2mg/ml) on the functionality of the plasma membrane of the Rhizopus stolonifer was studied. This study focuses on the changes in the integrity of the plasma membrane, external minimum medium pH, membrane potential, potassium efflux and determination of membrane phospholipids and proteins and of the H+-ATPase enzymatic kinetic activity. The results demonstrated that the membrane integrity diminishes gradually during 6h of incubation, that there was no change in the external minimum medium pH and that the spores treated with chitosan showed the lowest membrane potential compared with the control. The results also revealed an increase of five times of potassium efflux by the addition of chitosan. There were no significant observed differences in the content of total phospholipids in both treatments. However, protein content was reduced approximately 40% and total H+-ATPase activity decreased 52% in the presence of chitosan. Chitosan treatments diminished the kinetic parameters (Vmax and Km) of the H+-ATPase activity. The damage to the plasma membrane of R. stolonifer by the presence of chitosan alters the H+-ATPase, affecting the physiological and metabolic functions of this phytopathogen fungus.
AB - The effect of chitosan (2mg/ml) on the functionality of the plasma membrane of the Rhizopus stolonifer was studied. This study focuses on the changes in the integrity of the plasma membrane, external minimum medium pH, membrane potential, potassium efflux and determination of membrane phospholipids and proteins and of the H+-ATPase enzymatic kinetic activity. The results demonstrated that the membrane integrity diminishes gradually during 6h of incubation, that there was no change in the external minimum medium pH and that the spores treated with chitosan showed the lowest membrane potential compared with the control. The results also revealed an increase of five times of potassium efflux by the addition of chitosan. There were no significant observed differences in the content of total phospholipids in both treatments. However, protein content was reduced approximately 40% and total H+-ATPase activity decreased 52% in the presence of chitosan. Chitosan treatments diminished the kinetic parameters (Vmax and Km) of the H+-ATPase activity. The damage to the plasma membrane of R. stolonifer by the presence of chitosan alters the H+-ATPase, affecting the physiological and metabolic functions of this phytopathogen fungus.
KW - Chitosan
KW - Enzymatic kinetic
KW - H-ATPase activity
KW - Membrane potential
KW - México
KW - Phospholipids
KW - Proteins
UR - http://www.scopus.com/inward/record.url?scp=79960029724&partnerID=8YFLogxK
U2 - 10.1111/j.1439-0434.2011.01802.x
DO - 10.1111/j.1439-0434.2011.01802.x
M3 - Artículo
SN - 0931-1785
VL - 159
SP - 563
EP - 568
JO - Journal of Phytopathology
JF - Journal of Phytopathology
IS - 7-8
ER -