Changes in the functionality of plasma membrane of rhizopus stolonifer by addition of chitosan

Ana N. Hernández-Lauzardo, Josam Vega-Pérez, Miguel G. Velázquez-del Valle, Norma S. Sánchez, Antonio Peña, Guadalupe Guerra-Sánchez

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The effect of chitosan (2mg/ml) on the functionality of the plasma membrane of the Rhizopus stolonifer was studied. This study focuses on the changes in the integrity of the plasma membrane, external minimum medium pH, membrane potential, potassium efflux and determination of membrane phospholipids and proteins and of the H+-ATPase enzymatic kinetic activity. The results demonstrated that the membrane integrity diminishes gradually during 6h of incubation, that there was no change in the external minimum medium pH and that the spores treated with chitosan showed the lowest membrane potential compared with the control. The results also revealed an increase of five times of potassium efflux by the addition of chitosan. There were no significant observed differences in the content of total phospholipids in both treatments. However, protein content was reduced approximately 40% and total H+-ATPase activity decreased 52% in the presence of chitosan. Chitosan treatments diminished the kinetic parameters (Vmax and Km) of the H+-ATPase activity. The damage to the plasma membrane of R. stolonifer by the presence of chitosan alters the H+-ATPase, affecting the physiological and metabolic functions of this phytopathogen fungus. © 2011 Blackwell Verlag GmbH.
Original languageAmerican English
Pages (from-to)563-568
Number of pages506
JournalJournal of Phytopathology
DOIs
StatePublished - 1 Aug 2011

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Rhizopus stolonifer
Rhizopus
Chitosan
chitosan
H-transporting ATP synthase
Proton-Translocating ATPases
plasma membrane
Cell Membrane
membrane potential
Membrane Potentials
Phospholipids
Potassium
phospholipids
potassium
kinetics
Spores
plant pathogens
Membrane Proteins
Fungi
spores

Cite this

@article{347da09968b04975ad4625f0b4a20131,
title = "Changes in the functionality of plasma membrane of rhizopus stolonifer by addition of chitosan",
abstract = "The effect of chitosan (2mg/ml) on the functionality of the plasma membrane of the Rhizopus stolonifer was studied. This study focuses on the changes in the integrity of the plasma membrane, external minimum medium pH, membrane potential, potassium efflux and determination of membrane phospholipids and proteins and of the H+-ATPase enzymatic kinetic activity. The results demonstrated that the membrane integrity diminishes gradually during 6h of incubation, that there was no change in the external minimum medium pH and that the spores treated with chitosan showed the lowest membrane potential compared with the control. The results also revealed an increase of five times of potassium efflux by the addition of chitosan. There were no significant observed differences in the content of total phospholipids in both treatments. However, protein content was reduced approximately 40{\%} and total H+-ATPase activity decreased 52{\%} in the presence of chitosan. Chitosan treatments diminished the kinetic parameters (Vmax and Km) of the H+-ATPase activity. The damage to the plasma membrane of R. stolonifer by the presence of chitosan alters the H+-ATPase, affecting the physiological and metabolic functions of this phytopathogen fungus. {\circledC} 2011 Blackwell Verlag GmbH.",
author = "Hern{\'a}ndez-Lauzardo, {Ana N.} and Josam Vega-P{\'e}rez and {Vel{\'a}zquez-del Valle}, {Miguel G.} and S{\'a}nchez, {Norma S.} and Antonio Pe{\~n}a and Guadalupe Guerra-S{\'a}nchez",
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Changes in the functionality of plasma membrane of rhizopus stolonifer by addition of chitosan. / Hernández-Lauzardo, Ana N.; Vega-Pérez, Josam; Velázquez-del Valle, Miguel G.; Sánchez, Norma S.; Peña, Antonio; Guerra-Sánchez, Guadalupe.

In: Journal of Phytopathology, 01.08.2011, p. 563-568.

Research output: Contribution to journalArticle

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