TY - JOUR
T1 - Changes in biomass allocation and phenolic compounds accumulation due to the effect of light and nitrate supply in Cecropia peltata plants
AU - Izquierdo, Alejandro Mora
AU - Torres, María del Pilar Nicasio
AU - Sepúlveda Jiménez, Gabriela
AU - Sosa, Francisco Cruz
N1 - Funding Information:
This research was partially supported by a scholarship from the Consejo Nacional de Ciencia y Tecnología-México (CONACyT-México) (CVU 42514) and from the Instituto Mexicano del Seguro Social (IMSS) (99183755).
PY - 2011/11
Y1 - 2011/11
N2 - Cecropia peltata is popularly known as "guarumbo" in Mexico and is used in traditional medicine for treatment of diabetes mellitus. C. peltata plants were cultivated in a hydroponic system under controlled conditions. Gradients of light (20, 30 and 100 μmol m-2 s-1) and nitrate concentrations (13, 2 and 0. 2 mM) were applied to estimate their effect on biomass allocation and accumulation of bioactive (chlorogenic acid and isoorientin) phenolic compounds over a 28-day period. According to carbon nutrient balance (CNB) hypothesis predictions, biomass accumulation in foliage was stimulated by the highest irradiance (100 μmol m-2 s-1); similarly, at highest irradiance in combination with lowest nitrate concentration (0. 2 mM), root growth was stimulated (root-to-shoot ratio increased twofold with respect to the control). In these conditions, total phenolics (TP) and chlorogenic acid (CGA) contents were higher in aerial parts than in roots, with a 3. 8-fold increase in TP and a 7. 7-fold increase in CGA in foliage with respect to the control plants. Isoorientin was accumulated at very low levels. Antioxidant activity and total phenolic content showed a strong positive correlation. Phenylalanine ammonia-lyase activity (PAL) in aerial parts exhibited significant changes (&twofold) by highest irradiance. C. peltata plants allocate biomass and/or phenolic compounds to compensate the oxidative damage (increase in MDA levels) due to changes in light and nitrate restriction. The results are the basis for the establishment of a system of C. peltata culture in view of the potential use of C. peltata in therapeutic preparations for the treatment of diabetes mellitus.
AB - Cecropia peltata is popularly known as "guarumbo" in Mexico and is used in traditional medicine for treatment of diabetes mellitus. C. peltata plants were cultivated in a hydroponic system under controlled conditions. Gradients of light (20, 30 and 100 μmol m-2 s-1) and nitrate concentrations (13, 2 and 0. 2 mM) were applied to estimate their effect on biomass allocation and accumulation of bioactive (chlorogenic acid and isoorientin) phenolic compounds over a 28-day period. According to carbon nutrient balance (CNB) hypothesis predictions, biomass accumulation in foliage was stimulated by the highest irradiance (100 μmol m-2 s-1); similarly, at highest irradiance in combination with lowest nitrate concentration (0. 2 mM), root growth was stimulated (root-to-shoot ratio increased twofold with respect to the control). In these conditions, total phenolics (TP) and chlorogenic acid (CGA) contents were higher in aerial parts than in roots, with a 3. 8-fold increase in TP and a 7. 7-fold increase in CGA in foliage with respect to the control plants. Isoorientin was accumulated at very low levels. Antioxidant activity and total phenolic content showed a strong positive correlation. Phenylalanine ammonia-lyase activity (PAL) in aerial parts exhibited significant changes (&twofold) by highest irradiance. C. peltata plants allocate biomass and/or phenolic compounds to compensate the oxidative damage (increase in MDA levels) due to changes in light and nitrate restriction. The results are the basis for the establishment of a system of C. peltata culture in view of the potential use of C. peltata in therapeutic preparations for the treatment of diabetes mellitus.
KW - Antioxidant
KW - Chlorogenic acid
KW - Hydroponics
KW - Irradiance
KW - Nitrate
KW - PAL
KW - Phenolics
UR - http://www.scopus.com/inward/record.url?scp=85028102812&partnerID=8YFLogxK
U2 - 10.1007/s11738-011-0753-5
DO - 10.1007/s11738-011-0753-5
M3 - Artículo
SN - 0137-5881
VL - 33
SP - 2135
EP - 2147
JO - Acta Physiologiae Plantarum
JF - Acta Physiologiae Plantarum
IS - 6
ER -