Cell vacuolation induced by Haemophilus influenzae supernatants in HEp-2 cells

María del Rosario Espinoza-Mellado; Edgar Oliver López-Villegas; Ramón I. Arteaga-Garibay; Silvia Giono-Cerezo

doi:10.1590/0074-0276130716

Cell vacuolation induced by Haemophilus influenzae supernatants in HEp-2 cells

María del Rosario Espinoza-Mellado, Edgar Oliver López-Villegas, Ramón I. Arteaga-Garibay, Silvia Giono-Cerezo

Escuela Nacional de Ciencias Biológicas (ENCB)

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Haemophilus influenzae belongs to respiratory tract microbiota. We observed vacuoles formation in previous studies with H. influenzae culture supernatants, so in this work we characterised that cytotoxic effect. We observed an abundant production of acidic cytoplasmic vacuoles due to the presence of a "vacuolating factor" in H. influenzae supernatants which was characterised as thermolabile. Greatest vacuolating activity was observed when utilizing the fraction > 50 kDa. The presence of a large number of vacuoles in HEp-2 cells was verified by transmission electron microscopy and some vacuoles were identified with a double membrane and/or being surrounded by ribosomes. These results suggest similar behaviour to that of vacuolating effects described by autotransporter proteins an undescribed cytotoxic effect induced by H. influenzae.

Original language	English
Pages (from-to)	1074-1077
Number of pages	4
Journal	Memorias do Instituto Oswaldo Cruz
Volume	108
Issue number	8
DOIs	https://doi.org/10.1590/0074-0276130716
State	Published - 2013

Keywords

Cell vacuolation
Cytotoxicity assays
Haemophilus influenzae

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title = "Cell vacuolation induced by Haemophilus influenzae supernatants in HEp-2 cells",

abstract = "Haemophilus influenzae belongs to respiratory tract microbiota. We observed vacuoles formation in previous studies with H. influenzae culture supernatants, so in this work we characterised that cytotoxic effect. We observed an abundant production of acidic cytoplasmic vacuoles due to the presence of a {"}vacuolating factor{"} in H. influenzae supernatants which was characterised as thermolabile. Greatest vacuolating activity was observed when utilizing the fraction > 50 kDa. The presence of a large number of vacuoles in HEp-2 cells was verified by transmission electron microscopy and some vacuoles were identified with a double membrane and/or being surrounded by ribosomes. These results suggest similar behaviour to that of vacuolating effects described by autotransporter proteins an undescribed cytotoxic effect induced by H. influenzae.",

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T1 - Cell vacuolation induced by Haemophilus influenzae supernatants in HEp-2 cells

AU - Espinoza-Mellado, María del Rosario

AU - López-Villegas, Edgar Oliver

AU - Arteaga-Garibay, Ramón I.

AU - Giono-Cerezo, Silvia

PY - 2013

Y1 - 2013

N2 - Haemophilus influenzae belongs to respiratory tract microbiota. We observed vacuoles formation in previous studies with H. influenzae culture supernatants, so in this work we characterised that cytotoxic effect. We observed an abundant production of acidic cytoplasmic vacuoles due to the presence of a "vacuolating factor" in H. influenzae supernatants which was characterised as thermolabile. Greatest vacuolating activity was observed when utilizing the fraction > 50 kDa. The presence of a large number of vacuoles in HEp-2 cells was verified by transmission electron microscopy and some vacuoles were identified with a double membrane and/or being surrounded by ribosomes. These results suggest similar behaviour to that of vacuolating effects described by autotransporter proteins an undescribed cytotoxic effect induced by H. influenzae.

AB - Haemophilus influenzae belongs to respiratory tract microbiota. We observed vacuoles formation in previous studies with H. influenzae culture supernatants, so in this work we characterised that cytotoxic effect. We observed an abundant production of acidic cytoplasmic vacuoles due to the presence of a "vacuolating factor" in H. influenzae supernatants which was characterised as thermolabile. Greatest vacuolating activity was observed when utilizing the fraction > 50 kDa. The presence of a large number of vacuoles in HEp-2 cells was verified by transmission electron microscopy and some vacuoles were identified with a double membrane and/or being surrounded by ribosomes. These results suggest similar behaviour to that of vacuolating effects described by autotransporter proteins an undescribed cytotoxic effect induced by H. influenzae.

KW - Cell vacuolation

KW - Cytotoxicity assays

KW - Haemophilus influenzae

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DO - 10.1590/0074-0276130716

M3 - Artículo

SN - 0074-0276

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JO - Memorias do Instituto Oswaldo Cruz

JF - Memorias do Instituto Oswaldo Cruz

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Cell vacuolation induced by Haemophilus influenzae supernatants in HEp-2 cells

Abstract

Keywords

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