TY - JOUR
T1 - Cell culture of bursera linanoe in a stirred tank bioreactor for production of linalool and linalyl acetate
AU - Pavón-Reyes, Leticia
AU - Evangelista-Lozano, Silvia
AU - Sepúlveda-Jiménez, Gabriela
AU - Avila, Víctor Chávez
AU - Rodríguez-Monroy, Mario
PY - 2017/3
Y1 - 2017/3
N2 - Bursera linanoe cell suspension cultures were initiated from callus grown in Murashige and Skoog medium supplemented with naphthalene acetic acid (3.0 mg L-1) and 6-benzylaminopurine (0.5 mg L-1). In flasks, B. linanoe cell cultures grew over a 9 day period, reaching a maximum biomass of 11.16 g DW L-1. Throughout the growth phase, cell viability was constant at 60 - 70%. In contrast, B. linanoe cells growing in a bioreactor achieved a maximum biomass of 22.26 g DW L-1 (after 7 days), and cell viability was constant at 75 - 85%. Production of linalool and linalyl acetate in the bioreactor (3.02 and 2.40 mg g-1 DW, respectively) was significantly greater than that achieved from cells in flask cultures (1.05 and 0.97 mg g-1 DW, respectively). B. linanoe cell suspension culture has potential as an alternative method for the production of essential oils.
AB - Bursera linanoe cell suspension cultures were initiated from callus grown in Murashige and Skoog medium supplemented with naphthalene acetic acid (3.0 mg L-1) and 6-benzylaminopurine (0.5 mg L-1). In flasks, B. linanoe cell cultures grew over a 9 day period, reaching a maximum biomass of 11.16 g DW L-1. Throughout the growth phase, cell viability was constant at 60 - 70%. In contrast, B. linanoe cells growing in a bioreactor achieved a maximum biomass of 22.26 g DW L-1 (after 7 days), and cell viability was constant at 75 - 85%. Production of linalool and linalyl acetate in the bioreactor (3.02 and 2.40 mg g-1 DW, respectively) was significantly greater than that achieved from cells in flask cultures (1.05 and 0.97 mg g-1 DW, respectively). B. linanoe cell suspension culture has potential as an alternative method for the production of essential oils.
KW - Bursera linanoe
KW - Callus
KW - Cell viability
KW - Essential oils
KW - Monoterpene
UR - http://www.scopus.com/inward/record.url?scp=85019134807&partnerID=8YFLogxK
U2 - 10.1177/1934578x1701200301
DO - 10.1177/1934578x1701200301
M3 - Artículo
SN - 1934-578X
VL - 12
SP - 319
EP - 322
JO - Natural Product Communications
JF - Natural Product Communications
IS - 3
ER -