TY - JOUR
T1 - CD80 expression correlates with IL-6 production in THP-1-like macrophages costimulated with LPS and dialyzable leukocyte extract (Transferon®)
AU - Jiménez-Uribe, Alexis P.
AU - Valencia-Martínez, Hugo
AU - Carballo-Uicab, Gregorio
AU - Vallejo-Castillo, Luis
AU - Medina-Rivero, Emilio
AU - Chacón-Salinas, Rommel
AU - Pavón, Lenin
AU - Velasco-Velázquez, Marco A.
AU - Mellado-Sánchez, Gabriela
AU - Estrada-Parra, Sergio
AU - Pérez-Tapia, Sonia M.
N1 - Publisher Copyright:
Copyright © 2019 Alexis P. Jiménez-Uribe et al.
PY - 2019
Y1 - 2019
N2 - Transferon® is a complex drug based on a mixture of low molecular weight peptides. This biotherapeutic is employed as a coadjuvant in clinical trials of several diseases, including viral infections and allergies. Given that macrophages play key roles in pathogen recognition, phagocytosis, processing, and antigen presentation, we evaluated the effect of Transferon® on phenotype and function of macrophage-like cells derived from THP-1 monocytes. We determined the surface expression of CD80 and CD86 by flow cytometry and IL-1β, TNF-α, and IL-6 levels by ELISA. Transferon® alone did not alter the steady state of PMAdifferentiated macrophage-like THP-1 cells. On the contrary, simultaneous stimulation of cells with Transferon® and LPS elicited a significant increase in CD80 (P ≤ 0 001) and CD86 (P ≤ 0 001) expression, as well as in IL-6 production (P ≤ 0 05) compared to the LPS control. CD80 expression and IL-6 production exhibited a positive correlation (r = 0 6, P ≤ 0 05) in cells exposed to Transferon® and LPS. Our results suggest that the administration of Transferon® induces the expression of costimulatory molecules and the secretion of cytokines in LPS-Activated macrophages. Further studies are necessary to determine the implication of these findings in the therapeutic properties of Transferon®.
AB - Transferon® is a complex drug based on a mixture of low molecular weight peptides. This biotherapeutic is employed as a coadjuvant in clinical trials of several diseases, including viral infections and allergies. Given that macrophages play key roles in pathogen recognition, phagocytosis, processing, and antigen presentation, we evaluated the effect of Transferon® on phenotype and function of macrophage-like cells derived from THP-1 monocytes. We determined the surface expression of CD80 and CD86 by flow cytometry and IL-1β, TNF-α, and IL-6 levels by ELISA. Transferon® alone did not alter the steady state of PMAdifferentiated macrophage-like THP-1 cells. On the contrary, simultaneous stimulation of cells with Transferon® and LPS elicited a significant increase in CD80 (P ≤ 0 001) and CD86 (P ≤ 0 001) expression, as well as in IL-6 production (P ≤ 0 05) compared to the LPS control. CD80 expression and IL-6 production exhibited a positive correlation (r = 0 6, P ≤ 0 05) in cells exposed to Transferon® and LPS. Our results suggest that the administration of Transferon® induces the expression of costimulatory molecules and the secretion of cytokines in LPS-Activated macrophages. Further studies are necessary to determine the implication of these findings in the therapeutic properties of Transferon®.
UR - http://www.scopus.com/inward/record.url?scp=85066864621&partnerID=8YFLogxK
U2 - 10.1155/2019/2198508
DO - 10.1155/2019/2198508
M3 - Artículo
C2 - 31093509
AN - SCOPUS:85066864621
SN - 2314-8861
VL - 2019
JO - Journal of Immunology Research
JF - Journal of Immunology Research
M1 - 2198508
ER -