TY - JOUR
T1 - CD133+CD34+ and CD133+CD38+ blood progenitor cells as predictors of platelet engraftment in patients undergoing autologous peripheral blood stem cell transplantation
AU - Camacho Villa, Alma Y.
AU - Reyes Maldonado, Elba
AU - Montiel Cervantes, Laura A.
AU - Vela Ojeda, Jorge
N1 - Funding Information:
This work was supported in part by a grant from Fundación Gonzalo Río Arronte I.A.P. and by a grant from SIP-IPN 20100898.
PY - 2012/6
Y1 - 2012/6
N2 - Background: Hematopoietic stem cells (HSC) have been characterized by CD34+ expression and an adequate dose of CD34+ cells is associated with a complete engraftment. CD133 is a more specific marker of HSC. Materials and methods: We studied the relationship between graft content of CD34+, CD133+, and CD38+ cells and trilineage engraftment after autologous stem cell transplantation in patients with different hematological disorders. Blood samples were obtained before and after mobilization with recombinant granulocyte-colony stimulating factor (G-CSF, 16 μg/kg), from apheresis collections, and after transplantation. Results: Cell subsets were quantified by flow cytometry, and the dose of each population infused was correlated with success of engraftment. G-CSF induced mobilization of CD133+CD38+ cells (12.6-fold) and CD133+CD34+ cells (14.7-fold). A correlation was observed between the infused dose of CD133+CD34+ and CD133+CD38+ cells and platelet engraftment. Conclusion: CD133+CD34+ and CD133+CD38+ cells were mobilized with G-CSF and these cell subsets were correlated with platelet engraftment.
AB - Background: Hematopoietic stem cells (HSC) have been characterized by CD34+ expression and an adequate dose of CD34+ cells is associated with a complete engraftment. CD133 is a more specific marker of HSC. Materials and methods: We studied the relationship between graft content of CD34+, CD133+, and CD38+ cells and trilineage engraftment after autologous stem cell transplantation in patients with different hematological disorders. Blood samples were obtained before and after mobilization with recombinant granulocyte-colony stimulating factor (G-CSF, 16 μg/kg), from apheresis collections, and after transplantation. Results: Cell subsets were quantified by flow cytometry, and the dose of each population infused was correlated with success of engraftment. G-CSF induced mobilization of CD133+CD38+ cells (12.6-fold) and CD133+CD34+ cells (14.7-fold). A correlation was observed between the infused dose of CD133+CD34+ and CD133+CD38+ cells and platelet engraftment. Conclusion: CD133+CD34+ and CD133+CD38+ cells were mobilized with G-CSF and these cell subsets were correlated with platelet engraftment.
KW - Autologous transplantation
KW - CD133+CD34+ cells
KW - CD133+CD38+ cells
KW - Engraftment
KW - Stem cells
UR - http://www.scopus.com/inward/record.url?scp=84860442677&partnerID=8YFLogxK
U2 - 10.1016/j.transci.2012.02.002
DO - 10.1016/j.transci.2012.02.002
M3 - Artículo
C2 - 22386660
SN - 1473-0502
VL - 46
SP - 239
EP - 244
JO - Transfusion and Apheresis Science
JF - Transfusion and Apheresis Science
IS - 3
ER -