TY - JOUR
T1 - Catecholamine levels and gene expression of their receptors in tissues of adults with osteosarcoma
AU - Bandala, Cindy
AU - Ávila-Luna, Alberto
AU - Gómez-López, Modesto
AU - Estrada-Villaseñor, Eréndira
AU - Montes, Sergio
AU - Alfaro-Rodríguez, Alfonso
AU - Miliar-García, Ángel
AU - Cortés-Altamirano, José L.
AU - Peralta, Raúl
AU - Ibáñez-Cervantes, Gabriela
AU - Gómez-Manzo, Saúl
AU - Cárdenas-Rodríguez, Noemí
AU - Lara-Padilla, Eleazar
N1 - Publisher Copyright:
© 2019 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2021
Y1 - 2021
N2 - Aim: The purpose of this work was to identify and measure catecholamines, their metabolites, and the gene expression of catecholamine receptors in osteosarcoma tissue. Materials and methods: The levels of 3,4-dihydroxyphenylacetic acid, norepinephrine, serotonin, and 5-hydroxyindoleacetic acid in cancer tissue and in adjacent and non-oncological bone tissue were analysed by high-performance liquid chromatography, and the gene expression of catecholamine receptors and of dopamine β-hydroxylase, monoaminoxidase, ki67, and Runx2 in the osteosarcoma tissue, tissue adjacent to the tumour, non-oncological bone, and human brain tissue was analysed by RT-PCR. Results: We found significantly higher levels of 3,4-dihydroxyphenylacetic acid and norepinephrine in the cancer sample than in adjacent and non-oncological bone. We found that β-adrenergic receptors and dopaminergic receptors, dopamine β-hydroxylase, ki67, Runx2, and serotonergic receptor gene expression were significantly higher in tumour tissue than in adjacent and non-oncological bone. Conclusion: Catecholamines and their receptors could be potential molecular markers for osteosarcoma progression.
AB - Aim: The purpose of this work was to identify and measure catecholamines, their metabolites, and the gene expression of catecholamine receptors in osteosarcoma tissue. Materials and methods: The levels of 3,4-dihydroxyphenylacetic acid, norepinephrine, serotonin, and 5-hydroxyindoleacetic acid in cancer tissue and in adjacent and non-oncological bone tissue were analysed by high-performance liquid chromatography, and the gene expression of catecholamine receptors and of dopamine β-hydroxylase, monoaminoxidase, ki67, and Runx2 in the osteosarcoma tissue, tissue adjacent to the tumour, non-oncological bone, and human brain tissue was analysed by RT-PCR. Results: We found significantly higher levels of 3,4-dihydroxyphenylacetic acid and norepinephrine in the cancer sample than in adjacent and non-oncological bone. We found that β-adrenergic receptors and dopaminergic receptors, dopamine β-hydroxylase, ki67, Runx2, and serotonergic receptor gene expression were significantly higher in tumour tissue than in adjacent and non-oncological bone. Conclusion: Catecholamines and their receptors could be potential molecular markers for osteosarcoma progression.
KW - Dopamine
KW - catecholamines
KW - norepinephrine
KW - osteosarcoma
KW - serotonin
UR - http://www.scopus.com/inward/record.url?scp=85068772660&partnerID=8YFLogxK
U2 - 10.1080/13813455.2019.1638942
DO - 10.1080/13813455.2019.1638942
M3 - Artículo
C2 - 31291139
AN - SCOPUS:85068772660
SN - 1381-3455
VL - 127
SP - 337
EP - 343
JO - Archives of Physiology and Biochemistry
JF - Archives of Physiology and Biochemistry
IS - 4
ER -