TY - JOUR
T1 - Carotene-superproducing mutants ofPhycomyces blakesleeanus
AU - Salgado, Luis M.
AU - Bejarano, Eduardo R.
AU - Cerda-Olmedo, Enrique
N1 - Funding Information:
We thank A. Fernandez Estefane and 3. Cordoba for technical help and Hoffmann-LaRoche Company, Basel, and Comision Asesora para Investigation Cien-tifica y Ttcnica and Fondo de Investigaciones Sanita-rias de la Seguridad Social, Madrid, for financial support.
PY - 1989/12
Y1 - 1989/12
N2 - The wild-type mycelia of the fungusPhycomyces blakesleeanus are yellow because they contain small amounts of β-carotene. Some mutations lead to large increases in β-carotene content. These "deep yellow" mutants carry recessive mutations in either of two genes,carD andcarS, not linked to each other or to other genes related to carotenogenesis. ThecarS mutants contain up to 100 times more β-carotene than the wild type; thecarD mutants, up to 20 times.carS mutants are unable to form zygospores and their carotenogenesis is not activated by retinol; on the other hand,carD mutants complete the sexual cycle and respond to retinol.carS mutations are epistatic overcarD mutations. The product of genecarS mediates the end-product regulation of the pathway; it is suggested that thecarD gene product increases the amount or the activity of thecarS gene product.
AB - The wild-type mycelia of the fungusPhycomyces blakesleeanus are yellow because they contain small amounts of β-carotene. Some mutations lead to large increases in β-carotene content. These "deep yellow" mutants carry recessive mutations in either of two genes,carD andcarS, not linked to each other or to other genes related to carotenogenesis. ThecarS mutants contain up to 100 times more β-carotene than the wild type; thecarD mutants, up to 20 times.carS mutants are unable to form zygospores and their carotenogenesis is not activated by retinol; on the other hand,carD mutants complete the sexual cycle and respond to retinol.carS mutations are epistatic overcarD mutations. The product of genecarS mediates the end-product regulation of the pathway; it is suggested that thecarD gene product increases the amount or the activity of thecarS gene product.
KW - Phycomyces blakesleeanus β-carotene biosynthesis and regulation
KW - deep yellow mutants
UR - http://www.scopus.com/inward/record.url?scp=0024789884&partnerID=8YFLogxK
U2 - 10.1016/0147-5975(89)90028-5
DO - 10.1016/0147-5975(89)90028-5
M3 - Artículo
AN - SCOPUS:0024789884
SN - 0147-5975
VL - 13
SP - 332
EP - 336
JO - Experimental Mycology
JF - Experimental Mycology
IS - 4
ER -