Calpain-10 gene polymorphisms and risk of type 2 diabetes mellitus in Mexican mestizos

V. J. Picos-Cárdenas, E. Sáinz-González, A. Miliar-García, A. Romero-Zazueta, R. Quintero-Osuna, E. Leal-Ugarte, V. Peralta-Leal, J. P. Meza-Espinoza

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Abstract

© FUNPEC-RP. The calpain-10 gene is expressed primarily in tissues important in glucose metabolism; thus, some of its polymorphisms have been associated with type 2 diabetes. In this study, we examined the association between the calpain-10 single-nucleotide polymorphism (SNP)-43, SNP-19, and SNP-63 and type 2 diabetes in Mexican mestizos. We included 211 patients and 152 non-diabetic subjects. Polymerase chain reaction was used to identify alleles. We compared allele, genotype, haplotype, and diplotype frequencies between both groups and used the chi-square test to calculate the risk. The allele frequency of SNP-43 allele 1 was 70% in controls and 72% in patients; the GG, GA, and AA genotype frequencies were 48.7, 42.8, and 8.5% in controls and 51.2, 41.7, and 7.1% in patients, respectively. For SNP-19, the prevalence of allele 1 (2R) was 32% in controls and 39% in patients. In controls, homozygosity (2R/2R) was 10.5%, heterozygosity was 42.8%, and 3R/3R was 46.7%; in cases, these values were 13.3, 50.7, and 36.0%, respectively. For SNP-63, the frequency of allele 1 was 87% in controls and 83% in patients; genotype frequencies in controls were 75.7% (CC), 23% (CT), and 1.3% (TT), and were 69.7, 27.5, and 2.8%, respectively for the cases. Genotype distributions were consistent with Hardy-Weinberg equilibrium. No significant intergroup differences for allele, genotype, haplotype, or diplotype frequencies were observed. We found no association between these polymorphisms and diabetes. However, our sample size was small, so the role of calpain-10 risk alleles should be further examined.
Original languageAmerican English
Pages (from-to)2205-2215
Number of pages11
JournalGenetics and Molecular Research
DOIs
StatePublished - 27 Mar 2015

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Type 2 Diabetes Mellitus
Single Nucleotide Polymorphism
Alleles
Genotype
Genes
Gene Frequency
Haplotypes
Chi-Square Distribution
Sample Size
calpain 10
Glucose
Polymerase Chain Reaction

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Picos-Cárdenas, V. J., Sáinz-González, E., Miliar-García, A., Romero-Zazueta, A., Quintero-Osuna, R., Leal-Ugarte, E., ... Meza-Espinoza, J. P. (2015). Calpain-10 gene polymorphisms and risk of type 2 diabetes mellitus in Mexican mestizos. Genetics and Molecular Research, 2205-2215. https://doi.org/10.4238/2015.March.27.6
Picos-Cárdenas, V. J. ; Sáinz-González, E. ; Miliar-García, A. ; Romero-Zazueta, A. ; Quintero-Osuna, R. ; Leal-Ugarte, E. ; Peralta-Leal, V. ; Meza-Espinoza, J. P. / Calpain-10 gene polymorphisms and risk of type 2 diabetes mellitus in Mexican mestizos. In: Genetics and Molecular Research. 2015 ; pp. 2205-2215.
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abstract = "{\circledC} FUNPEC-RP. The calpain-10 gene is expressed primarily in tissues important in glucose metabolism; thus, some of its polymorphisms have been associated with type 2 diabetes. In this study, we examined the association between the calpain-10 single-nucleotide polymorphism (SNP)-43, SNP-19, and SNP-63 and type 2 diabetes in Mexican mestizos. We included 211 patients and 152 non-diabetic subjects. Polymerase chain reaction was used to identify alleles. We compared allele, genotype, haplotype, and diplotype frequencies between both groups and used the chi-square test to calculate the risk. The allele frequency of SNP-43 allele 1 was 70{\%} in controls and 72{\%} in patients; the GG, GA, and AA genotype frequencies were 48.7, 42.8, and 8.5{\%} in controls and 51.2, 41.7, and 7.1{\%} in patients, respectively. For SNP-19, the prevalence of allele 1 (2R) was 32{\%} in controls and 39{\%} in patients. In controls, homozygosity (2R/2R) was 10.5{\%}, heterozygosity was 42.8{\%}, and 3R/3R was 46.7{\%}; in cases, these values were 13.3, 50.7, and 36.0{\%}, respectively. For SNP-63, the frequency of allele 1 was 87{\%} in controls and 83{\%} in patients; genotype frequencies in controls were 75.7{\%} (CC), 23{\%} (CT), and 1.3{\%} (TT), and were 69.7, 27.5, and 2.8{\%}, respectively for the cases. Genotype distributions were consistent with Hardy-Weinberg equilibrium. No significant intergroup differences for allele, genotype, haplotype, or diplotype frequencies were observed. We found no association between these polymorphisms and diabetes. However, our sample size was small, so the role of calpain-10 risk alleles should be further examined.",
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Picos-Cárdenas, VJ, Sáinz-González, E, Miliar-García, A, Romero-Zazueta, A, Quintero-Osuna, R, Leal-Ugarte, E, Peralta-Leal, V & Meza-Espinoza, JP 2015, 'Calpain-10 gene polymorphisms and risk of type 2 diabetes mellitus in Mexican mestizos', Genetics and Molecular Research, pp. 2205-2215. https://doi.org/10.4238/2015.March.27.6

Calpain-10 gene polymorphisms and risk of type 2 diabetes mellitus in Mexican mestizos. / Picos-Cárdenas, V. J.; Sáinz-González, E.; Miliar-García, A.; Romero-Zazueta, A.; Quintero-Osuna, R.; Leal-Ugarte, E.; Peralta-Leal, V.; Meza-Espinoza, J. P.

In: Genetics and Molecular Research, 27.03.2015, p. 2205-2215.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Calpain-10 gene polymorphisms and risk of type 2 diabetes mellitus in Mexican mestizos

AU - Picos-Cárdenas, V. J.

AU - Sáinz-González, E.

AU - Miliar-García, A.

AU - Romero-Zazueta, A.

AU - Quintero-Osuna, R.

AU - Leal-Ugarte, E.

AU - Peralta-Leal, V.

AU - Meza-Espinoza, J. P.

PY - 2015/3/27

Y1 - 2015/3/27

N2 - © FUNPEC-RP. The calpain-10 gene is expressed primarily in tissues important in glucose metabolism; thus, some of its polymorphisms have been associated with type 2 diabetes. In this study, we examined the association between the calpain-10 single-nucleotide polymorphism (SNP)-43, SNP-19, and SNP-63 and type 2 diabetes in Mexican mestizos. We included 211 patients and 152 non-diabetic subjects. Polymerase chain reaction was used to identify alleles. We compared allele, genotype, haplotype, and diplotype frequencies between both groups and used the chi-square test to calculate the risk. The allele frequency of SNP-43 allele 1 was 70% in controls and 72% in patients; the GG, GA, and AA genotype frequencies were 48.7, 42.8, and 8.5% in controls and 51.2, 41.7, and 7.1% in patients, respectively. For SNP-19, the prevalence of allele 1 (2R) was 32% in controls and 39% in patients. In controls, homozygosity (2R/2R) was 10.5%, heterozygosity was 42.8%, and 3R/3R was 46.7%; in cases, these values were 13.3, 50.7, and 36.0%, respectively. For SNP-63, the frequency of allele 1 was 87% in controls and 83% in patients; genotype frequencies in controls were 75.7% (CC), 23% (CT), and 1.3% (TT), and were 69.7, 27.5, and 2.8%, respectively for the cases. Genotype distributions were consistent with Hardy-Weinberg equilibrium. No significant intergroup differences for allele, genotype, haplotype, or diplotype frequencies were observed. We found no association between these polymorphisms and diabetes. However, our sample size was small, so the role of calpain-10 risk alleles should be further examined.

AB - © FUNPEC-RP. The calpain-10 gene is expressed primarily in tissues important in glucose metabolism; thus, some of its polymorphisms have been associated with type 2 diabetes. In this study, we examined the association between the calpain-10 single-nucleotide polymorphism (SNP)-43, SNP-19, and SNP-63 and type 2 diabetes in Mexican mestizos. We included 211 patients and 152 non-diabetic subjects. Polymerase chain reaction was used to identify alleles. We compared allele, genotype, haplotype, and diplotype frequencies between both groups and used the chi-square test to calculate the risk. The allele frequency of SNP-43 allele 1 was 70% in controls and 72% in patients; the GG, GA, and AA genotype frequencies were 48.7, 42.8, and 8.5% in controls and 51.2, 41.7, and 7.1% in patients, respectively. For SNP-19, the prevalence of allele 1 (2R) was 32% in controls and 39% in patients. In controls, homozygosity (2R/2R) was 10.5%, heterozygosity was 42.8%, and 3R/3R was 46.7%; in cases, these values were 13.3, 50.7, and 36.0%, respectively. For SNP-63, the frequency of allele 1 was 87% in controls and 83% in patients; genotype frequencies in controls were 75.7% (CC), 23% (CT), and 1.3% (TT), and were 69.7, 27.5, and 2.8%, respectively for the cases. Genotype distributions were consistent with Hardy-Weinberg equilibrium. No significant intergroup differences for allele, genotype, haplotype, or diplotype frequencies were observed. We found no association between these polymorphisms and diabetes. However, our sample size was small, so the role of calpain-10 risk alleles should be further examined.

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Picos-Cárdenas VJ, Sáinz-González E, Miliar-García A, Romero-Zazueta A, Quintero-Osuna R, Leal-Ugarte E et al. Calpain-10 gene polymorphisms and risk of type 2 diabetes mellitus in Mexican mestizos. Genetics and Molecular Research. 2015 Mar 27;2205-2215. https://doi.org/10.4238/2015.March.27.6