TY - JOUR
T1 - Biosurfactant Production by Bacillus tequilensis ZSB10
T2 - Structural Characterization, Physicochemical, and Antifungal Properties
AU - Cortés-Camargo, S.
AU - Acuña-Avila, P. E.
AU - Arrieta-Báez, D.
AU - Montañez-Barragán, B.
AU - Morato, A. I.
AU - Sanz-Martín, J. L.
AU - Barragán-Huerta, B. E.
N1 - Publisher Copyright:
© 2021 AOCS.
PY - 2021/9
Y1 - 2021/9
N2 - A new biosurfactant was obtained from a moderately halophilic bacterium identified as Bacillus tequilensis ZSB10 that was isolated from a saline water pond located in Tehuacan-Cuicatlan valley, Mexico. A kinetic analysis of the bacterial growth of the ZSB10 strain showed a maximum growth at 24 h regardless of the initial pH (5, 7.4, and 9). The best results were found at pH = 7.4 in terms of bacterial growth, besides which the produced biosurfactant showed emulsifying and surfactant properties with an emulsification index (E24) and surface tension change (ΔST) of 54 ± 0% and 26 mN m−1, respectively. Extracted ZSB10 crude biosurfactant had a yield of 106 ± 6 mg L−1, an E24 = 58.4 ± 0.2%, and a ΔST = 26 mN m−1 with a critical micelle concentration (CMC) of 44.82 mg L−1. Also, its structure was characterized by MALDI-TOF mass spectrometry as a surfactin, iturin A, and fengycin mixture whose main isoform was leu/ile-7 C15 surfactin [M + Na]+. Finally, the ZSB10 crude biosurfactant showed antifungal activity against Helminthosporium sp., with a 79.3% growth inhibition and an IC50 of 1.37 mg per disc. Therefore, this biosurfactant could be used as biopesticide.
AB - A new biosurfactant was obtained from a moderately halophilic bacterium identified as Bacillus tequilensis ZSB10 that was isolated from a saline water pond located in Tehuacan-Cuicatlan valley, Mexico. A kinetic analysis of the bacterial growth of the ZSB10 strain showed a maximum growth at 24 h regardless of the initial pH (5, 7.4, and 9). The best results were found at pH = 7.4 in terms of bacterial growth, besides which the produced biosurfactant showed emulsifying and surfactant properties with an emulsification index (E24) and surface tension change (ΔST) of 54 ± 0% and 26 mN m−1, respectively. Extracted ZSB10 crude biosurfactant had a yield of 106 ± 6 mg L−1, an E24 = 58.4 ± 0.2%, and a ΔST = 26 mN m−1 with a critical micelle concentration (CMC) of 44.82 mg L−1. Also, its structure was characterized by MALDI-TOF mass spectrometry as a surfactin, iturin A, and fengycin mixture whose main isoform was leu/ile-7 C15 surfactin [M + Na]+. Finally, the ZSB10 crude biosurfactant showed antifungal activity against Helminthosporium sp., with a 79.3% growth inhibition and an IC50 of 1.37 mg per disc. Therefore, this biosurfactant could be used as biopesticide.
KW - Antifungal properties
KW - Bacillus tequilensis
KW - Emulsifying properties
KW - Iturin
KW - Surfactant properties
KW - Surfactin
UR - http://www.scopus.com/inward/record.url?scp=85104527708&partnerID=8YFLogxK
U2 - 10.1002/jsde.12515
DO - 10.1002/jsde.12515
M3 - Artículo
AN - SCOPUS:85104527708
SN - 1097-3958
VL - 24
SP - 773
EP - 782
JO - Journal of Surfactants and Detergents
JF - Journal of Surfactants and Detergents
IS - 5
ER -