Bioprospecting for fungi with potential pathogenic activity on leaves of Agave tequilana Weber var. Azul

Gerardo Campos-Rivero, Lorenzo F. Sánchez-Teyer, Erika A. De la Cruz-Arguijo, Maria S. Ramírez-González, Claudia P. Larralde-Corona, José A. Narváez-Zapata

Research output: Contribution to journalArticle

Abstract

Thirty different fungal strains were isolated from A. tequilana leaves showing disease symptoms such as wilt and curled leaves, black, red and chlorotic spots. Ten genera were identified and confirmed by using the LSU D1/D2 rDNA and ITS1-5.8S-ITS2 regions, mainly of the Ascomycota phylum, where the Lasiodiploidia and Neoscytalidium genera were the more (46.6%) abundant. The other genera identified were Cladosporium, Cytospora, Epicoccum, Flavodon, Lasiodiplodia, Myrmaecium, Neoscytalidium, Penicillium, Peniophora, Purpureocillium, Trametes and Fusarium. Five strains of Lasiodiplodia and one of Fusarium were selected based on their representativeness and pathogenic potential on Agaves. Pathogenic potential was analysed by both, an infection assay, evidenced as necrosis, and by pectinolytic activity. Specifically, necrosis infection assay was conducted by puncture (wounded) infection and by direct mycelium contact. In general, Lasiodiplodia strains exhibited different pathogenic profiles according to their necrosis percentages, regardless of the infection method used. Fusarium strain analysed also showed a high necrosis infection (> 99%). Pectinolytic activity used as an indirect measurement of pathogenesis presented a high Fusarium extract activity (peaking at 23.9 U). Lasiodiplodia strains exhibited up 6 times more enzymatic activity (peaking at 143.5) than Fusarium strain analysed. In addition, Agave leaf extracts used totally or partially as carbon source during fungal induction culture may induce different pathogenic activities in these strains. In general, the two pathogenicity assays implemented evidenced differences in the pathogenicity profile of these analysed strains.

Original languageEnglish
Pages (from-to)283-294
Number of pages12
JournalJournal of Phytopathology
Volume167
Issue number5
DOIs
StatePublished - May 2019

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Pinta
Agave
Agave tequilana
Fusarium
Fungi
Necrosis
fungi
Infection
leaves
necrosis
Neoscytalidium
pathogenicity
Virulence
infection
Trametes
Cladosporium
Ascomycota
Penicillium
Mycelium
Ribosomal DNA

Keywords

  • Agave
  • fungal community
  • pathogenic potential

Cite this

Campos-Rivero, Gerardo ; Sánchez-Teyer, Lorenzo F. ; De la Cruz-Arguijo, Erika A. ; Ramírez-González, Maria S. ; Larralde-Corona, Claudia P. ; Narváez-Zapata, José A. / Bioprospecting for fungi with potential pathogenic activity on leaves of Agave tequilana Weber var. Azul. In: Journal of Phytopathology. 2019 ; Vol. 167, No. 5. pp. 283-294.
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Bioprospecting for fungi with potential pathogenic activity on leaves of Agave tequilana Weber var. Azul. / Campos-Rivero, Gerardo; Sánchez-Teyer, Lorenzo F.; De la Cruz-Arguijo, Erika A.; Ramírez-González, Maria S.; Larralde-Corona, Claudia P.; Narváez-Zapata, José A.

In: Journal of Phytopathology, Vol. 167, No. 5, 05.2019, p. 283-294.

Research output: Contribution to journalArticle

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T1 - Bioprospecting for fungi with potential pathogenic activity on leaves of Agave tequilana Weber var. Azul

AU - Campos-Rivero, Gerardo

AU - Sánchez-Teyer, Lorenzo F.

AU - De la Cruz-Arguijo, Erika A.

AU - Ramírez-González, Maria S.

AU - Larralde-Corona, Claudia P.

AU - Narváez-Zapata, José A.

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