Binding and potential antibiofilm activities of Amaranthus proteins against Candida albicans

Rajamohamed Beema Shafreen, Siddharthan Seema, Alma Leticia Martinez-Ayala, María A. Lozano-Grande, Maribel Robles-Sánchez, Arkadiusz Szterk, Michael Grishko, Ezra Hanuka, Elena Katrich, Shela Gorinstein

Research output: Contribution to journalArticle

Abstract

In the present study evaluation of structural, thermal and antifungal properties of Amaranthus hypochondriacus laboratory protein isolate (ALMA) and commercially available Amaranthus protein dietary antidepressant (APGM) was done by differential scanning calorimetry (DSC), Fourier Transform Infrared (FTIR) and fluorescence spectroscopy and antibiofilm activities against Candida albicans. The results exhibited thermal stability and antioxidant activity for the isolates. Fluorescence measurements showed that they bind to human serum albumin through a static quenching mechanism, decreasing its fluorescence intensity. FTIR spectra showed amides I, II and III shifts, but it does not modify the structural and bioactive properties against C. albicans despite of its infections which is difficult to treat due to virulence expression and biofilm formation that protects of therapeutic drugs. Both isolates had the potential to assuage two virulence factors such as biofilm formation and yeast to hyphal transition of C. albicans. The biofilm inhibitory concentration of the protein isolates was determined to 10 and 30 μg mL−1 with 50% inhibition, while morphogenic transition of the yeast leads to host tissue damage was significantly inhibited in spider medium and in vivo assay with zebrafish embryo. Inhibition of C. albicans biofilm by protein isolates was well compared with COMSTAT and XTT assay. The conformational changes in the proteins of investigated samples were determined by fluorescence after denaturation with 8 M urea and showed slight differences in comparison with the natural product. This is the first study to envisage the use of amaranth protein isolates to immunocompromised patients in their diet plan that can prevent C. albicans infections and help them in recovery. These isolates can be used as natural polymers in biomedical applications and edible films for health benefits.

Original languageEnglish
Article number110479
JournalColloids and Surfaces B: Biointerfaces
Volume183
DOIs
StatePublished - 1 Nov 2019

Fingerprint

Amaranthus
Candida
Candida albicans
Biofilms
biofilms
proteins
Proteins
virulence
Fluorescence
fluorescence
Yeast
yeast
infectious diseases
Assays
Hot Temperature
Yeasts
Natural polymers
Spiders
Denaturation
Dietary Proteins

Keywords

  • Antibiofilm activity
  • Binding and thermostability properties
  • Biomedical polymer
  • Candida albicans
  • Conformational changes
  • Human serum albumin
  • Protein isolates

Cite this

Beema Shafreen, Rajamohamed ; Seema, Siddharthan ; Martinez-Ayala, Alma Leticia ; Lozano-Grande, María A. ; Robles-Sánchez, Maribel ; Szterk, Arkadiusz ; Grishko, Michael ; Hanuka, Ezra ; Katrich, Elena ; Gorinstein, Shela. / Binding and potential antibiofilm activities of Amaranthus proteins against Candida albicans. In: Colloids and Surfaces B: Biointerfaces. 2019 ; Vol. 183.
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Beema Shafreen, R, Seema, S, Martinez-Ayala, AL, Lozano-Grande, MA, Robles-Sánchez, M, Szterk, A, Grishko, M, Hanuka, E, Katrich, E & Gorinstein, S 2019, 'Binding and potential antibiofilm activities of Amaranthus proteins against Candida albicans', Colloids and Surfaces B: Biointerfaces, vol. 183, 110479. https://doi.org/10.1016/j.colsurfb.2019.110479

Binding and potential antibiofilm activities of Amaranthus proteins against Candida albicans. / Beema Shafreen, Rajamohamed; Seema, Siddharthan; Martinez-Ayala, Alma Leticia; Lozano-Grande, María A.; Robles-Sánchez, Maribel; Szterk, Arkadiusz; Grishko, Michael; Hanuka, Ezra; Katrich, Elena; Gorinstein, Shela.

In: Colloids and Surfaces B: Biointerfaces, Vol. 183, 110479, 01.11.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Binding and potential antibiofilm activities of Amaranthus proteins against Candida albicans

AU - Beema Shafreen, Rajamohamed

AU - Seema, Siddharthan

AU - Martinez-Ayala, Alma Leticia

AU - Lozano-Grande, María A.

AU - Robles-Sánchez, Maribel

AU - Szterk, Arkadiusz

AU - Grishko, Michael

AU - Hanuka, Ezra

AU - Katrich, Elena

AU - Gorinstein, Shela

PY - 2019/11/1

Y1 - 2019/11/1

N2 - In the present study evaluation of structural, thermal and antifungal properties of Amaranthus hypochondriacus laboratory protein isolate (ALMA) and commercially available Amaranthus protein dietary antidepressant (APGM) was done by differential scanning calorimetry (DSC), Fourier Transform Infrared (FTIR) and fluorescence spectroscopy and antibiofilm activities against Candida albicans. The results exhibited thermal stability and antioxidant activity for the isolates. Fluorescence measurements showed that they bind to human serum albumin through a static quenching mechanism, decreasing its fluorescence intensity. FTIR spectra showed amides I, II and III shifts, but it does not modify the structural and bioactive properties against C. albicans despite of its infections which is difficult to treat due to virulence expression and biofilm formation that protects of therapeutic drugs. Both isolates had the potential to assuage two virulence factors such as biofilm formation and yeast to hyphal transition of C. albicans. The biofilm inhibitory concentration of the protein isolates was determined to 10 and 30 μg mL−1 with 50% inhibition, while morphogenic transition of the yeast leads to host tissue damage was significantly inhibited in spider medium and in vivo assay with zebrafish embryo. Inhibition of C. albicans biofilm by protein isolates was well compared with COMSTAT and XTT assay. The conformational changes in the proteins of investigated samples were determined by fluorescence after denaturation with 8 M urea and showed slight differences in comparison with the natural product. This is the first study to envisage the use of amaranth protein isolates to immunocompromised patients in their diet plan that can prevent C. albicans infections and help them in recovery. These isolates can be used as natural polymers in biomedical applications and edible films for health benefits.

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KW - Antibiofilm activity

KW - Binding and thermostability properties

KW - Biomedical polymer

KW - Candida albicans

KW - Conformational changes

KW - Human serum albumin

KW - Protein isolates

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JO - Colloids and Surfaces B: Biointerfaces

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