Beta vulgaris L. suspension cultures permeabilized with Triton X-100 retain cell viability and betacyanines production ability: A digital image analysis study

Gabriela Trejo-Tapia; Jonathan Cuevas-Celis; Guadalupe Salcedo-Morales; José Luis Trejo-Espino; Martha L. Arenas-Ocampo; Antonio Jiménez-Aparicio

doi:10.1021/bp0602597

Beta vulgaris L. suspension cultures permeabilized with Triton X-100 retain cell viability and betacyanines production ability: A digital image analysis study

Gabriela Trejo-Tapia, Jonathan Cuevas-Celis, Guadalupe Salcedo-Morales, José Luis Trejo-Espino, Martha L. Arenas-Ocampo, Antonio Jiménez-Aparicio

Centro de Desarrollo de Productos Bióticos (CEPROBI)

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18 Scopus citations

Abstract

The influence of Triton X-100 on Beta vulgaris L. permeabilized cell culture viability, regrowth, and ability to produce betacyanines was evaluated in this study. A non-destructive method based on the analysis of images in the RGB (red, green, blue) system was developed to estimate betacyanines content. A treatment for 15 min with 0.7 mM Triton X-100 induced the release of 30% of betacyanines without loss of cell viability (≥70%). After this permeabilization treatment, B. vulgaris cultures regrew normally, reaching a maximum biomass concentration of 48% higher than non-permeabilized cultures after 14 days of culture. Also, maximum betacyanines concentration was only 25% lower than that of non-permeabilized cultures.

Original language	English
Pages (from-to)	359-363
Number of pages	5
Journal	Biotechnology Progress
Volume	23
Issue number	2
DOIs	https://doi.org/10.1021/bp0602597
State	Published - Mar 2007

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10.1021/bp0602597

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title = "Beta vulgaris L. suspension cultures permeabilized with Triton X-100 retain cell viability and betacyanines production ability: A digital image analysis study",

abstract = "The influence of Triton X-100 on Beta vulgaris L. permeabilized cell culture viability, regrowth, and ability to produce betacyanines was evaluated in this study. A non-destructive method based on the analysis of images in the RGB (red, green, blue) system was developed to estimate betacyanines content. A treatment for 15 min with 0.7 mM Triton X-100 induced the release of 30% of betacyanines without loss of cell viability (≥70%). After this permeabilization treatment, B. vulgaris cultures regrew normally, reaching a maximum biomass concentration of 48% higher than non-permeabilized cultures after 14 days of culture. Also, maximum betacyanines concentration was only 25% lower than that of non-permeabilized cultures.",

author = "Gabriela Trejo-Tapia and Jonathan Cuevas-Celis and Guadalupe Salcedo-Morales and Trejo-Espino, {Jos{\'e} Luis} and Arenas-Ocampo, {Martha L.} and Antonio Jim{\'e}nez-Aparicio",

year = "2007",

month = mar,

doi = "10.1021/bp0602597",

language = "Ingl{\'e}s",

volume = "23",

pages = "359--363",

journal = "Biotechnology Progress",

issn = "8756-7938",

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Beta vulgaris L. suspension cultures permeabilized with Triton X-100 retain cell viability and betacyanines production ability: A digital image analysis study. / Trejo-Tapia, Gabriela; Cuevas-Celis, Jonathan; Salcedo-Morales, Guadalupe et al.
In: Biotechnology Progress, Vol. 23, No. 2, 03.2007, p. 359-363.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Beta vulgaris L. suspension cultures permeabilized with Triton X-100 retain cell viability and betacyanines production ability

T2 - A digital image analysis study

AU - Trejo-Tapia, Gabriela

AU - Cuevas-Celis, Jonathan

AU - Salcedo-Morales, Guadalupe

AU - Trejo-Espino, José Luis

AU - Arenas-Ocampo, Martha L.

AU - Jiménez-Aparicio, Antonio

PY - 2007/3

Y1 - 2007/3

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AB - The influence of Triton X-100 on Beta vulgaris L. permeabilized cell culture viability, regrowth, and ability to produce betacyanines was evaluated in this study. A non-destructive method based on the analysis of images in the RGB (red, green, blue) system was developed to estimate betacyanines content. A treatment for 15 min with 0.7 mM Triton X-100 induced the release of 30% of betacyanines without loss of cell viability (≥70%). After this permeabilization treatment, B. vulgaris cultures regrew normally, reaching a maximum biomass concentration of 48% higher than non-permeabilized cultures after 14 days of culture. Also, maximum betacyanines concentration was only 25% lower than that of non-permeabilized cultures.

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JO - Biotechnology Progress

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Beta vulgaris L. suspension cultures permeabilized with Triton X-100 retain cell viability and betacyanines production ability: A digital image analysis study

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