Beta vulgaris L. suspension cultures permeabilized with Triton X-100 retain cell viability and betacyanines production ability: A digital image analysis study

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The influence of Triton X-100 on Beta vulgaris L. permeabilized cell culture viability, regrowth, and ability to produce betacyanines was evaluated in this study. A non-destructive method based on the analysis of images in the RGB (red, green, blue) system was developed to estimate betacyanines content. A treatment for 15 min with 0.7 mM Triton X-100 induced the release of 30% of betacyanines without loss of cell viability (≥70%). After this permeabilization treatment, B. vulgaris cultures regrew normally, reaching a maximum biomass concentration of 48% higher than non-permeabilized cultures after 14 days of culture. Also, maximum betacyanines concentration was only 25% lower than that of non-permeabilized cultures. © 2007 American Chemical Society and American Institute of Chemical Engineers.
Original languageAmerican English
Pages (from-to)359-363
Number of pages322
JournalBiotechnology Progress
DOIs
StatePublished - 1 Mar 2007

Fingerprint

Beta vulgaris
Octoxynol
Cell culture
Image analysis
Cell Survival
Suspensions
Biomass
Cells
Engineers
Cell Culture Techniques

Cite this

@article{c7d4305dd58f4734911b8f28c0842943,
title = "Beta vulgaris L. suspension cultures permeabilized with Triton X-100 retain cell viability and betacyanines production ability: A digital image analysis study",
abstract = "The influence of Triton X-100 on Beta vulgaris L. permeabilized cell culture viability, regrowth, and ability to produce betacyanines was evaluated in this study. A non-destructive method based on the analysis of images in the RGB (red, green, blue) system was developed to estimate betacyanines content. A treatment for 15 min with 0.7 mM Triton X-100 induced the release of 30{\%} of betacyanines without loss of cell viability (≥70{\%}). After this permeabilization treatment, B. vulgaris cultures regrew normally, reaching a maximum biomass concentration of 48{\%} higher than non-permeabilized cultures after 14 days of culture. Also, maximum betacyanines concentration was only 25{\%} lower than that of non-permeabilized cultures. {\circledC} 2007 American Chemical Society and American Institute of Chemical Engineers.",
author = "Gabriela Trejo-Tapia and Jonathan Cuevas-Celis and Guadalupe Salcedo-Morales and Trejo-Espino, {Jos{\'e} Luis} and Arenas-Ocampo, {Martha L.} and Antonio Jim{\'e}nez-Aparicio",
year = "2007",
month = "3",
day = "1",
doi = "10.1021/bp0602597",
language = "American English",
pages = "359--363",
journal = "Biotechnology Progress",
issn = "8756-7938",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Beta vulgaris L. suspension cultures permeabilized with Triton X-100 retain cell viability and betacyanines production ability: A digital image analysis study

AU - Trejo-Tapia, Gabriela

AU - Cuevas-Celis, Jonathan

AU - Salcedo-Morales, Guadalupe

AU - Trejo-Espino, José Luis

AU - Arenas-Ocampo, Martha L.

AU - Jiménez-Aparicio, Antonio

PY - 2007/3/1

Y1 - 2007/3/1

N2 - The influence of Triton X-100 on Beta vulgaris L. permeabilized cell culture viability, regrowth, and ability to produce betacyanines was evaluated in this study. A non-destructive method based on the analysis of images in the RGB (red, green, blue) system was developed to estimate betacyanines content. A treatment for 15 min with 0.7 mM Triton X-100 induced the release of 30% of betacyanines without loss of cell viability (≥70%). After this permeabilization treatment, B. vulgaris cultures regrew normally, reaching a maximum biomass concentration of 48% higher than non-permeabilized cultures after 14 days of culture. Also, maximum betacyanines concentration was only 25% lower than that of non-permeabilized cultures. © 2007 American Chemical Society and American Institute of Chemical Engineers.

AB - The influence of Triton X-100 on Beta vulgaris L. permeabilized cell culture viability, regrowth, and ability to produce betacyanines was evaluated in this study. A non-destructive method based on the analysis of images in the RGB (red, green, blue) system was developed to estimate betacyanines content. A treatment for 15 min with 0.7 mM Triton X-100 induced the release of 30% of betacyanines without loss of cell viability (≥70%). After this permeabilization treatment, B. vulgaris cultures regrew normally, reaching a maximum biomass concentration of 48% higher than non-permeabilized cultures after 14 days of culture. Also, maximum betacyanines concentration was only 25% lower than that of non-permeabilized cultures. © 2007 American Chemical Society and American Institute of Chemical Engineers.

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34247178569&origin=inward

UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=34247178569&origin=inward

U2 - 10.1021/bp0602597

DO - 10.1021/bp0602597

M3 - Article

C2 - 17253724

SP - 359

EP - 363

JO - Biotechnology Progress

JF - Biotechnology Progress

SN - 8756-7938

ER -