TY - JOUR
T1 - Bacteriological quality of bottled water obtained from Mexico City small water purification plants
T2 - Incidence and identification of potentially pathogenic nontuberculous mycobacteria species
AU - Cerna-Cortes, Jorge Francisco
AU - Cortes-Cueto, Ana Laura
AU - Villegas-Martínez, Daniel
AU - Leon-Montes, Nancy
AU - Salas-Rangel, Laura P.
AU - Rivera-Gutierrez, Sandra
AU - Lopez-Hernandez, Daniel
AU - Helguera-Repetto, Addy Cecilia
AU - Fernandez-Rendon, Elizabeth
AU - Gonzalez-y-Merchand, Jorge Alberto
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/10/2
Y1 - 2019/10/2
N2 - The aim of this study was to determine the bacteriological quality of bottled water samples obtained from small purification plants located in Mexico City and to identify potentially pathogenic nontuberculous mycobacteria (NTM) species found in these samples. All 111 samples analyzed were positive for aerobic mesophilic bacteria (AMB) and 46 (41.4%) did not comply with Mexico's Official Guidelines. Sixty-nine (62.1%) and 23 (20.7%) water samples were positive for total coliforms (TC) and fecal coliforms (FC), respectively. A total of 81 (72.9%) of the water samples exceeded the maximum allowed limit stipulated in the guideline. Thirty-three (29.7%) of the purified water samples were positive for NTM, being recovered a total of 40 isolates. These NTM isolates were identified using three molecular markers (hsp65, rrs and rpoB genes) which corresponded to the fast-growing mycobacteria M. chelonae (n = 12), M. porcinum (n = 8), M. senegalense (n = 5), M. abscessus (n = 4), M. septicum (n = 4), M. wolinskyi (n = 3), M. mucogenicum (n = 2), M. fortuitum (n = 1) and M. sp. (n = 1). In seven purified water samples, two different NTM species were isolated simultaneously. Overall, these results showed that most of the purified bottled water samples analyzed in this study had unsatisfactory microbiological quality and some harbored NTM associated with illness. Our data could hasten health authorities to intensify efforts in the routine monitoring of activities in the purified bottled water industry in order to supply safe and healthy water to the public.
AB - The aim of this study was to determine the bacteriological quality of bottled water samples obtained from small purification plants located in Mexico City and to identify potentially pathogenic nontuberculous mycobacteria (NTM) species found in these samples. All 111 samples analyzed were positive for aerobic mesophilic bacteria (AMB) and 46 (41.4%) did not comply with Mexico's Official Guidelines. Sixty-nine (62.1%) and 23 (20.7%) water samples were positive for total coliforms (TC) and fecal coliforms (FC), respectively. A total of 81 (72.9%) of the water samples exceeded the maximum allowed limit stipulated in the guideline. Thirty-three (29.7%) of the purified water samples were positive for NTM, being recovered a total of 40 isolates. These NTM isolates were identified using three molecular markers (hsp65, rrs and rpoB genes) which corresponded to the fast-growing mycobacteria M. chelonae (n = 12), M. porcinum (n = 8), M. senegalense (n = 5), M. abscessus (n = 4), M. septicum (n = 4), M. wolinskyi (n = 3), M. mucogenicum (n = 2), M. fortuitum (n = 1) and M. sp. (n = 1). In seven purified water samples, two different NTM species were isolated simultaneously. Overall, these results showed that most of the purified bottled water samples analyzed in this study had unsatisfactory microbiological quality and some harbored NTM associated with illness. Our data could hasten health authorities to intensify efforts in the routine monitoring of activities in the purified bottled water industry in order to supply safe and healthy water to the public.
KW - Microbiological quality
KW - Mycobacteria
KW - Purified water
UR - http://www.scopus.com/inward/record.url?scp=85068552780&partnerID=8YFLogxK
U2 - 10.1016/j.ijfoodmicro.2019.108260
DO - 10.1016/j.ijfoodmicro.2019.108260
M3 - Artículo
C2 - 31302488
AN - SCOPUS:85068552780
SN - 0168-1605
VL - 306
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
M1 - 108260
ER -