TY - JOUR
T1 - Bacterial communities associated with the rhizosphere of pioneer plants (Bahia xylopoda and Viguiera linearis) growing on heavy metals-contaminated soils
AU - Navarro-Noya, Yendi E.
AU - Jan-Roblero, Janet
AU - González-Chávez, Del Carmen
AU - Hernández-Gama, Regina
AU - Hernández-Rodríguez, César
N1 - Funding Information:
Acknowledgments This work was supported by grants SIP20070651, SIP20070164, SIP20080688 and SIP20080628 Instituto Politécnico Nacional (IPN). Y. E. Navarro-Noya thanks the Consejo Nacional de Ciencia y Tecnología (CONACyT) and the Programa Institucional de Formación de Investigadores (PIFI), IPN, for scholarships. C. Hernández-Rodríguez and J. Jan-Roblero appreciate the COFAA, and EDI, IPN fellowships; and SNI, CONACyT. Our special thanks to L. M. Diaz-Garduño for her valuable assistance with the vegetal species identification.
PY - 2010/5
Y1 - 2010/5
N2 - In this study, the bacterial communities associated with the rhizospheres of pioneer plants Bahia xylopoda and Viguiera linearis were explored. These plants grow on silver mine tailings with high concentration of heavy metals in Zacatecas, Mexico. Metagenomic DNAs from rhizosphere and bulk soil were extracted to perform a denaturing gradient gel electrophoresis analysis (DGGE) and to construct 16S rRNA gene libraries. A moderate bacterial diversity and twelve major phylogenetic groups including Proteobacteria, Acidobacteria, Bacteroidetes, Gemmatimonadetes, Chloroflexi, Firmicutes, Verrucomicrobia, Nitrospirae and Actinobacteria phyla, and divisions TM7, OP10 and OD1 were recognized in the rhizospheres. Only 25.5% from the phylotypes were common in the rhizosphere libraries and the most abundant groups were members of the phyla Acidobacteria and Betaproteobacteria (Thiobacillus spp., Nitrosomonadaceae). The most abundant groups in bulk soil library were Acidobacteria and Actinobacteria, and no common phylotypes were shared with the rhizosphere libraries. Many of the clones detected were related with chemolithotrophic and sulfur-oxidizing bacteria, characteristic of an environment with a high concentration of heavy metal-sulfur complexes, and lacking carbon and organic energy sources.
AB - In this study, the bacterial communities associated with the rhizospheres of pioneer plants Bahia xylopoda and Viguiera linearis were explored. These plants grow on silver mine tailings with high concentration of heavy metals in Zacatecas, Mexico. Metagenomic DNAs from rhizosphere and bulk soil were extracted to perform a denaturing gradient gel electrophoresis analysis (DGGE) and to construct 16S rRNA gene libraries. A moderate bacterial diversity and twelve major phylogenetic groups including Proteobacteria, Acidobacteria, Bacteroidetes, Gemmatimonadetes, Chloroflexi, Firmicutes, Verrucomicrobia, Nitrospirae and Actinobacteria phyla, and divisions TM7, OP10 and OD1 were recognized in the rhizospheres. Only 25.5% from the phylotypes were common in the rhizosphere libraries and the most abundant groups were members of the phyla Acidobacteria and Betaproteobacteria (Thiobacillus spp., Nitrosomonadaceae). The most abundant groups in bulk soil library were Acidobacteria and Actinobacteria, and no common phylotypes were shared with the rhizosphere libraries. Many of the clones detected were related with chemolithotrophic and sulfur-oxidizing bacteria, characteristic of an environment with a high concentration of heavy metal-sulfur complexes, and lacking carbon and organic energy sources.
KW - Bahia xylopoda
KW - Chemolithotrophic
KW - Heavy-metals
KW - Rhizosphere
KW - Viguiera linearis
UR - http://www.scopus.com/inward/record.url?scp=77951667084&partnerID=8YFLogxK
U2 - 10.1007/s10482-010-9413-9
DO - 10.1007/s10482-010-9413-9
M3 - Artículo
C2 - 20084459
SN - 0003-6072
VL - 97
SP - 335
EP - 349
JO - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
JF - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
IS - 4
ER -