TY - JOUR
T1 - B vitamins increase the analgesic effect of diclofenac in the rat
AU - Rocha-González, Hector I.
AU - Terán-Rosales, Flavio
AU - Reyes-García, Gerardo
AU - Medina-Santillán, Roberto
AU - Granados-Soto, Vinicio
PY - 2004
Y1 - 2004
N2 - The purpose of this study was to assess the possible synergistic interaction between diclofenac and B-vitamins (100:100:1 of vitamin B 1, B6 and B12, respectively) in the rat formalin test. Female Wistar rats were injected into the dorsal surface of the right hind paw with 50 λl of diluted formalin (1%). Reduction of the number of flinches was considered as antinociception. Diclofenac (0.31-316 mg/kg), B-vitamins (32-178 mg/kg), or a combination of B-vitamins and diclofenac was administered orally and the antinociceptive effect was determined, Isobolographic analyses were used to define the nature of the functional interactions between B-vitamins and diclofenac in two proportions (1:1, 1:3; Diclofenac:B-Vitamins). Diclofenac (ED30 3.7 ± 0.4 mg/kg), B-vitamins (ED30 76.2 ± 8.5 mg/kg), and fixed-ratio B-vitamins-diclofenac combinations dose-dependently reduced flinching behavior during second phase of the test. Theoretical ED30 values for the combination estimated from the isobolograms were 39.9 ± 4.2 and 58.1 ± 6.4 mg/kg for 1:1 and 1:3 proportions, respectively. These values were significantly higher than experimental ED30 values which were 7.4 ± 9.3 and 22.8 ± 9.8 mg/kg for 1:1 and 1:3 proportions, respectively. Results indicate that oral diclofenac and B-vitamins can interact synergistically to reduce inflammatory pain in the formalin test and suggest the use of those combinations to relief this kind of pain in humans.
AB - The purpose of this study was to assess the possible synergistic interaction between diclofenac and B-vitamins (100:100:1 of vitamin B 1, B6 and B12, respectively) in the rat formalin test. Female Wistar rats were injected into the dorsal surface of the right hind paw with 50 λl of diluted formalin (1%). Reduction of the number of flinches was considered as antinociception. Diclofenac (0.31-316 mg/kg), B-vitamins (32-178 mg/kg), or a combination of B-vitamins and diclofenac was administered orally and the antinociceptive effect was determined, Isobolographic analyses were used to define the nature of the functional interactions between B-vitamins and diclofenac in two proportions (1:1, 1:3; Diclofenac:B-Vitamins). Diclofenac (ED30 3.7 ± 0.4 mg/kg), B-vitamins (ED30 76.2 ± 8.5 mg/kg), and fixed-ratio B-vitamins-diclofenac combinations dose-dependently reduced flinching behavior during second phase of the test. Theoretical ED30 values for the combination estimated from the isobolograms were 39.9 ± 4.2 and 58.1 ± 6.4 mg/kg for 1:1 and 1:3 proportions, respectively. These values were significantly higher than experimental ED30 values which were 7.4 ± 9.3 and 22.8 ± 9.8 mg/kg for 1:1 and 1:3 proportions, respectively. Results indicate that oral diclofenac and B-vitamins can interact synergistically to reduce inflammatory pain in the formalin test and suggest the use of those combinations to relief this kind of pain in humans.
UR - http://www.scopus.com/inward/record.url?scp=9444228259&partnerID=8YFLogxK
M3 - Artículo
C2 - 15633620
AN - SCOPUS:9444228259
SN - 0083-8969
VL - 47
SP - 84
EP - 87
JO - Proceedings of the Western Pharmacology Society
JF - Proceedings of the Western Pharmacology Society
ER -