Attraction of adults of cyclocephala lunulata and cyclocephala barrerai (Coleoptera Scarabaeoidea: Melolonthidae) towards bacteria volatiles isolated from their genital chambers

Abraham Sanchez-Cruz, Norma Robledo, María Rosete-Enríquez, Angel A. Romero-López

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

In the study of the chemical communication of adults of the Melolonthidae family, bacteria have been observed in the epithelium of the genital chamber; possibly, bacteria are involved in the production of sex attractants in their hosts. Therefore, it is important to identify the volatile organic compounds from bacteria (VOCsB) released by these microorganisms and study the biological activity stimulated by VOBCs in adults of Melolonthidae. In this study, bacteria were isolated from the genital chamber of Cyclocephala lunulata and Cyclocephala barrerai, from which VOCsB were extracted using static headspace solid-phase microextraction (SHS-SPME) and dynamic headspace Super Q solid-phase extraction (DHS-SPE) and analyzed using gas chromatography-mass spectrometry. The effect of VOCsB on the hosts and conspecifics was evaluated utilizing an olfactometer and electroantennography (EAG). Two species of Enterobacteria were isolated from the genital chamber of each female species, and VOCsB derived from sulfur-containing compounds, alcohols, esters, and fatty acids were identified. An attraction response was observed in olfactometry studies, and antennal responses to VOCsB were confirmed in EAG bioassays. With these results, new perspectives on the relationship between these beetles and their bacteria emerge, in addition to establishing a basis for management programs in the future.

Original languageEnglish
Article number4430
JournalMolecules
Volume25
Issue number19
DOIs
StatePublished - Oct 2020

Keywords

  • Attraction
  • Bioassays
  • Cyclocephala
  • EAG
  • Microbial natural products discovery
  • Volatile organic compounds from bacteria

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