ATOMIC FORCE MICROSCOPY PRINCIPLES AND RECENT STUDIES OF IMAGING AND NANOMECHANICAL PROPERTIES IN BACTERIA

H. H. Torres-Ventura, J. J. Chanona-Pérez, L. Dorantes-Álvarez, J. V. Méndez-Méndez, B. Arredondo-Tamayo, P. I. Cauich-Sánchez, Ana Elena Jiménez-Carmona

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations

Abstract

There are two main modes in where it operates, imaging and force spectroscopy; the first one is divided into three major mode categories, which are contact, non-contact, and intermittent mode and can be adapted for air or liquid media. The tapping method is more suitable for imaging of soft biological samples and with low surface adhesion such as DNA (deoxyribonucleic acid) chains, biopolymers, bacteria, yeast, cells, and tissues of animal and vegetable origin. In addition, soft surface imaging conditions can be maintained for long periods of time, allowing continuous imaging of bacterial cell growth and division, even on planar substrates. Although AFM techniques are already widely used, their applications and tools continue to grow with the development of new methodologies to acquisition of bacterial imaging at high resolution and force spectroscopy with very fast scanning.

Original languageEnglish
Title of host publicationBiogenic Nanomaterials
Subtitle of host publicationStructural Properties and Functional Applications
PublisherApple Academic Press
Pages49-82
Number of pages34
ISBN (Electronic)9781000565140
ISBN (Print)9781774638385
DOIs
StatePublished - 1 Jan 2022

Keywords

  • PeakForce tapping
  • bacterial imaging
  • cantilever tip
  • force spectroscopy
  • high-speed AFM
  • immobilization
  • nanoindentation
  • nanomechanical properties
  • quantitative imaging
  • quantitative nanomechanics

Fingerprint

Dive into the research topics of 'ATOMIC FORCE MICROSCOPY PRINCIPLES AND RECENT STUDIES OF IMAGING AND NANOMECHANICAL PROPERTIES IN BACTERIA'. Together they form a unique fingerprint.

Cite this