Associations of TNFA, IL17A, and RORC mRNA expression levels in peripheral blood leukocytes with obesity-related asthma in adolescents

José J. Leija-Martínez; Blanca E. Del-Río-Navarro; Fausto Sanchéz-Muñoz; Onofre Muñoz-Hernández; Enrique Hong; Abraham Giacoman-Martínez; Rodrigo Romero-Nava; Karla L. Patricio-Román; Margareth S. Hall-Mondragon; Dario Espinosa-Velazquez; Santiago Villafaña; Fengyang Huang

doi:10.1016/j.clim.2021.108715

Associations of TNFA, IL17A, and RORC mRNA expression levels in peripheral blood leukocytes with obesity-related asthma in adolescents

José J. Leija-Martínez, Blanca E. Del-Río-Navarro, Fausto Sanchéz-Muñoz, Onofre Muñoz-Hernández, Enrique Hong, Abraham Giacoman-Martínez, Rodrigo Romero-Nava, Karla L. Patricio-Román, Margareth S. Hall-Mondragon, Dario Espinosa-Velazquez, Santiago Villafaña, Fengyang Huang

Escuela Superior de Medicina (ESM)

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Obesity is associated with a unique non-T2 asthma phenotype, characterised by a Th17 immune response. Retinoid-related orphan receptor C (RORC) is the master transcription factor for Th17 polarisation. We investigated the association of TNFA, IL17A, and RORC mRNA expression levels with the non-T2 phenotype. We conducted a cross-sectional study in adolescents, subdivided as follows: healthy (HA), allergic asthma without obesity (AA), obesity without asthma (OB), and non-allergic asthma with obesity (NAO). TNFA, IL17A, and RORC mRNA expression in peripheral blood leukocytes were assessed by RT-PCR. NAO exhibited higher TNFA mRNA expression levels than HA or OB, as well as the highest IL17A and RORC mRNA expression levels among the four groups. The best biomarker for discriminating non-allergic asthma among obese adolescents was RORC mRNA expression levels (area under the curve: 0.95). RORC mRNA expression levels were associated with the non-T2 asthma phenotype, hinting at a therapeutic target in obesity-related asthma.

Original language	English
Article number	108715
Journal	Clinical Immunology
Volume	229
DOIs	https://doi.org/10.1016/j.clim.2021.108715
State	Published - Aug 2021

Keywords

Asthma
IL-17A
Non-allergic asthma
Obesity
RORC
Tumour necrosis factor-alpha

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.clim.2021.108715

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Leija-Martínez, J. J., Del-Río-Navarro, B. E., Sanchéz-Muñoz, F., Muñoz-Hernández, O., Hong, E., Giacoman-Martínez, A., Romero-Nava, R., Patricio-Román, K. L., Hall-Mondragon, M. S., Espinosa-Velazquez, D., Villafaña, S., & Huang, F. (2021). Associations of TNFA, IL17A, and RORC mRNA expression levels in peripheral blood leukocytes with obesity-related asthma in adolescents. Clinical Immunology, 229, Article 108715. https://doi.org/10.1016/j.clim.2021.108715

@article{54fd65e5c31c400ba8d22033635312ef,

title = "Associations of TNFA, IL17A, and RORC mRNA expression levels in peripheral blood leukocytes with obesity-related asthma in adolescents",

abstract = "Obesity is associated with a unique non-T2 asthma phenotype, characterised by a Th17 immune response. Retinoid-related orphan receptor C (RORC) is the master transcription factor for Th17 polarisation. We investigated the association of TNFA, IL17A, and RORC mRNA expression levels with the non-T2 phenotype. We conducted a cross-sectional study in adolescents, subdivided as follows: healthy (HA), allergic asthma without obesity (AA), obesity without asthma (OB), and non-allergic asthma with obesity (NAO). TNFA, IL17A, and RORC mRNA expression in peripheral blood leukocytes were assessed by RT-PCR. NAO exhibited higher TNFA mRNA expression levels than HA or OB, as well as the highest IL17A and RORC mRNA expression levels among the four groups. The best biomarker for discriminating non-allergic asthma among obese adolescents was RORC mRNA expression levels (area under the curve: 0.95). RORC mRNA expression levels were associated with the non-T2 asthma phenotype, hinting at a therapeutic target in obesity-related asthma.",

keywords = "Asthma, IL-17A, Non-allergic asthma, Obesity, RORC, Tumour necrosis factor-alpha",

author = "Leija-Mart{\'i}nez, {Jos{\'e} J.} and Del-R{\'i}o-Navarro, {Blanca E.} and Fausto Sanch{\'e}z-Mu{\~n}oz and Onofre Mu{\~n}oz-Hern{\'a}ndez and Enrique Hong and Abraham Giacoman-Mart{\'i}nez and Rodrigo Romero-Nava and Patricio-Rom{\'a}n, {Karla L.} and Hall-Mondragon, {Margareth S.} and Dario Espinosa-Velazquez and Santiago Villafa{\~n}a and Fengyang Huang",

note = "Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",

year = "2021",

month = aug,

doi = "10.1016/j.clim.2021.108715",

language = "Ingl{\'e}s",

volume = "229",

journal = "Clinical Immunology",

issn = "1521-6616",

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Leija-Martínez, JJ, Del-Río-Navarro, BE, Sanchéz-Muñoz, F, Muñoz-Hernández, O, Hong, E, Giacoman-Martínez, A, Romero-Nava, R, Patricio-Román, KL, Hall-Mondragon, MS, Espinosa-Velazquez, D, Villafaña, S & Huang, F 2021, 'Associations of TNFA, IL17A, and RORC mRNA expression levels in peripheral blood leukocytes with obesity-related asthma in adolescents', Clinical Immunology, vol. 229, 108715. https://doi.org/10.1016/j.clim.2021.108715

TY - JOUR

T1 - Associations of TNFA, IL17A, and RORC mRNA expression levels in peripheral blood leukocytes with obesity-related asthma in adolescents

AU - Leija-Martínez, José J.

AU - Del-Río-Navarro, Blanca E.

AU - Sanchéz-Muñoz, Fausto

AU - Muñoz-Hernández, Onofre

AU - Hong, Enrique

AU - Giacoman-Martínez, Abraham

AU - Romero-Nava, Rodrigo

AU - Patricio-Román, Karla L.

AU - Hall-Mondragon, Margareth S.

AU - Espinosa-Velazquez, Dario

AU - Villafaña, Santiago

AU - Huang, Fengyang

PY - 2021/8

Y1 - 2021/8

N2 - Obesity is associated with a unique non-T2 asthma phenotype, characterised by a Th17 immune response. Retinoid-related orphan receptor C (RORC) is the master transcription factor for Th17 polarisation. We investigated the association of TNFA, IL17A, and RORC mRNA expression levels with the non-T2 phenotype. We conducted a cross-sectional study in adolescents, subdivided as follows: healthy (HA), allergic asthma without obesity (AA), obesity without asthma (OB), and non-allergic asthma with obesity (NAO). TNFA, IL17A, and RORC mRNA expression in peripheral blood leukocytes were assessed by RT-PCR. NAO exhibited higher TNFA mRNA expression levels than HA or OB, as well as the highest IL17A and RORC mRNA expression levels among the four groups. The best biomarker for discriminating non-allergic asthma among obese adolescents was RORC mRNA expression levels (area under the curve: 0.95). RORC mRNA expression levels were associated with the non-T2 asthma phenotype, hinting at a therapeutic target in obesity-related asthma.

AB - Obesity is associated with a unique non-T2 asthma phenotype, characterised by a Th17 immune response. Retinoid-related orphan receptor C (RORC) is the master transcription factor for Th17 polarisation. We investigated the association of TNFA, IL17A, and RORC mRNA expression levels with the non-T2 phenotype. We conducted a cross-sectional study in adolescents, subdivided as follows: healthy (HA), allergic asthma without obesity (AA), obesity without asthma (OB), and non-allergic asthma with obesity (NAO). TNFA, IL17A, and RORC mRNA expression in peripheral blood leukocytes were assessed by RT-PCR. NAO exhibited higher TNFA mRNA expression levels than HA or OB, as well as the highest IL17A and RORC mRNA expression levels among the four groups. The best biomarker for discriminating non-allergic asthma among obese adolescents was RORC mRNA expression levels (area under the curve: 0.95). RORC mRNA expression levels were associated with the non-T2 asthma phenotype, hinting at a therapeutic target in obesity-related asthma.

KW - Asthma

KW - IL-17A

KW - Non-allergic asthma

KW - Obesity

KW - RORC

KW - Tumour necrosis factor-alpha

UR - http://www.scopus.com/inward/record.url?scp=85108108134&partnerID=8YFLogxK

U2 - 10.1016/j.clim.2021.108715

DO - 10.1016/j.clim.2021.108715

M3 - Artículo

C2 - 33771687

AN - SCOPUS:85108108134

SN - 1521-6616

VL - 229

JO - Clinical Immunology

JF - Clinical Immunology

M1 - 108715

ER -

Associations of TNFA, IL17A, and RORC mRNA expression levels in peripheral blood leukocytes with obesity-related asthma in adolescents

Abstract

Keywords

UN SDGs

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