Association of genetic polymorphisms in tlr3, tlr4, tlr7, and tlr8 with the clinical forms of dengue in patients from veracruz, Mexico

Araceli Posadas-Mondragón, José Leopoldo Aguilar-Faisal, Gerardo Zuñiga, Jonathan Javier Magaña, José Angel Santiago-Cruz, Edith Guillén-Salomón, Verónica Alcántara-Farfán, María Luisa Arellano-Flores, Juan Santiago Salas-Benito, Rocío M. Neri-Bazán, Lucero Luna-Rojas, Amanda Marineth Avila-Trejo, Adolfo Chávez-Negrete

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Dengue manifestations range from a mild form, dengue fever (DF), to more severe forms such as dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The ability of the host to present one of these clinical forms could be related to polymorphisms located in genes of the Toll-like receptors (TLRs) which activate the pro-inflammatory response. Therefore, the genotyping of single nucleotide genetic polymorphisms (SNPs) in TLR3 (rs3775291 and rs6552950), TLR4 (rs2737190, rs10759932, rs4986790, rs4986791, rs11536865, and rs10983755), TLR7 (rs179008 and rs3853839), and TLR8 (rs3764880, rs5741883, rs4830805, and rs1548731) was carried out in non-genetically related DHF patients, DF patients, and general population (GP) subjects. The SNPs were analyzed by real-time PCR by genotyping assays from Applied Biosystems®. The codominance model showed that dengue patients had a lower probability of presenting the TLR4-rs2737190-G/G genotype (odds ratio (OR) (95% CI) = 0.34 (0.14–0.8), p = 0.038). Dengue patients showed a lower probability of presenting TLR4-rs11536865-G/C genotype (OR (95% CI) = 0.19 (0.05–0.73), p = 0.0092) and had a high probability of presenting the TACG haplotype, but lower probability of presenting the TGCG haplotype in the TLR4 compared to GP individuals (OR (95% CI) = 0.55 (0.35–0.86), p = 0.0084). In conclusion, the TLR4-rs2737190-G/G and TLR4-rs11536865-G/C genotypes and TGCG haplotype were associated with protection from dengue.

Original languageEnglish
Article number1230
JournalViruses
Volume12
Issue number11
DOIs
StatePublished - Nov 2020

Keywords

  • Dengue
  • Dengue fever
  • Dengue hemorrhagic fever
  • Gene polymorphisms
  • Human genetic susceptibility
  • Toll-like receptor

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