Association of Fusobacterium nucleatum infection and colorectal cancer: A Mexican study

H. Cuellar-Gómez; M. E. Ocharán-Hernández; C. C. Calzada-Mendoza; D. A. Comoto-Santacruz

doi:10.1016/j.rgmx.2020.12.002

Association of Fusobacterium nucleatum infection and colorectal cancer: A Mexican study

Translated title of the contribution: Association of Fusobacterium nucleatum infection and colorectal cancer: A Mexican study

H. Cuellar-Gómez, M. E. Ocharán-Hernández, C. C. Calzada-Mendoza, D. A. Comoto-Santacruz

Escuela Superior de Medicina (ESM)

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Introduction and aims: Colorectal cancer (CRC) is the third most prevalent cancer worldwide. Many risk factors are involved, and current evidence links the gut microbiota and colorectal carcinogenesis. Fusobacterium nucleatum is proposed as one of the risk factors at the onset and during the progression of CRC, due to immune system and inflammatory modulation. Materials and methods: Ninety samples from three different regions of the colon were collected through colonoscopy in patients with CRC, and qPCR TagMan® was conducted to detect F. nucleatum and cytokines (IL-17, IL-23, and IL-10) in tumor, peritumor, and normal samples. The differences between them were analyzed and correlated. Results: The abundance of F. nucleatum determined through the 2^-ΔΔCt method in CRC (7.750 [5.790-10.469]) was significantly higher than in the normal control (0.409 [0.251-0.817]) (p < 0.05). There was no significant association between F. nucleatum and the cytokines (p > 0.05). Conclusions: CRC is a heterogeneous disease that presents and progresses in a complex microenvironment, partially due to gut microbiome imbalance. F. nucleatum was enriched in CRC tissue, but whether that is a cause of the pathology or a consequence, has not yet been clearly defined.

Translated title of the contribution	Association of Fusobacterium nucleatum infection and colorectal cancer: A Mexican study
Original language	English
Pages (from-to)	277-284
Number of pages	8
Journal	Revista de Gastroenterologia de Mexico
Volume	87
Issue number	3
DOIs	https://doi.org/10.1016/j.rgmx.2020.12.002
State	Published - 1 Jul 2022

Keywords

Colorectal cancer
Cytokines
Fusobacterium nucleatum
Inflammation
Microbiota

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10.1016/j.rgmx.2020.12.002

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title = "Association of Fusobacterium nucleatum infection and colorectal cancer: A Mexican study",

abstract = "Introduction and aims: Colorectal cancer (CRC) is the third most prevalent cancer worldwide. Many risk factors are involved, and current evidence links the gut microbiota and colorectal carcinogenesis. Fusobacterium nucleatum is proposed as one of the risk factors at the onset and during the progression of CRC, due to immune system and inflammatory modulation. Materials and methods: Ninety samples from three different regions of the colon were collected through colonoscopy in patients with CRC, and qPCR TagMan{\textregistered} was conducted to detect F. nucleatum and cytokines (IL-17, IL-23, and IL-10) in tumor, peritumor, and normal samples. The differences between them were analyzed and correlated. Results: The abundance of F. nucleatum determined through the 2-ΔΔCt method in CRC (7.750 [5.790-10.469]) was significantly higher than in the normal control (0.409 [0.251-0.817]) (p < 0.05). There was no significant association between F. nucleatum and the cytokines (p > 0.05). Conclusions: CRC is a heterogeneous disease that presents and progresses in a complex microenvironment, partially due to gut microbiome imbalance. F. nucleatum was enriched in CRC tissue, but whether that is a cause of the pathology or a consequence, has not yet been clearly defined.",

keywords = "Colorectal cancer, Cytokines, Fusobacterium nucleatum, Inflammation, Microbiota",

author = "H. Cuellar-G{\'o}mez and Ochar{\'a}n-Hern{\'a}ndez, {M. E.} and Calzada-Mendoza, {C. C.} and Comoto-Santacruz, {D. A.}",

note = "Publisher Copyright: {\textcopyright} 2021 Asociaci{\'o}n Mexicana de Gastroenterolog{\'i}a",

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doi = "10.1016/j.rgmx.2020.12.002",

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T1 - Association of Fusobacterium nucleatum infection and colorectal cancer

T2 - A Mexican study

AU - Cuellar-Gómez, H.

AU - Ocharán-Hernández, M. E.

AU - Calzada-Mendoza, C. C.

AU - Comoto-Santacruz, D. A.

PY - 2022/7/1

Y1 - 2022/7/1

N2 - Introduction and aims: Colorectal cancer (CRC) is the third most prevalent cancer worldwide. Many risk factors are involved, and current evidence links the gut microbiota and colorectal carcinogenesis. Fusobacterium nucleatum is proposed as one of the risk factors at the onset and during the progression of CRC, due to immune system and inflammatory modulation. Materials and methods: Ninety samples from three different regions of the colon were collected through colonoscopy in patients with CRC, and qPCR TagMan® was conducted to detect F. nucleatum and cytokines (IL-17, IL-23, and IL-10) in tumor, peritumor, and normal samples. The differences between them were analyzed and correlated. Results: The abundance of F. nucleatum determined through the 2-ΔΔCt method in CRC (7.750 [5.790-10.469]) was significantly higher than in the normal control (0.409 [0.251-0.817]) (p < 0.05). There was no significant association between F. nucleatum and the cytokines (p > 0.05). Conclusions: CRC is a heterogeneous disease that presents and progresses in a complex microenvironment, partially due to gut microbiome imbalance. F. nucleatum was enriched in CRC tissue, but whether that is a cause of the pathology or a consequence, has not yet been clearly defined.

AB - Introduction and aims: Colorectal cancer (CRC) is the third most prevalent cancer worldwide. Many risk factors are involved, and current evidence links the gut microbiota and colorectal carcinogenesis. Fusobacterium nucleatum is proposed as one of the risk factors at the onset and during the progression of CRC, due to immune system and inflammatory modulation. Materials and methods: Ninety samples from three different regions of the colon were collected through colonoscopy in patients with CRC, and qPCR TagMan® was conducted to detect F. nucleatum and cytokines (IL-17, IL-23, and IL-10) in tumor, peritumor, and normal samples. The differences between them were analyzed and correlated. Results: The abundance of F. nucleatum determined through the 2-ΔΔCt method in CRC (7.750 [5.790-10.469]) was significantly higher than in the normal control (0.409 [0.251-0.817]) (p < 0.05). There was no significant association between F. nucleatum and the cytokines (p > 0.05). Conclusions: CRC is a heterogeneous disease that presents and progresses in a complex microenvironment, partially due to gut microbiome imbalance. F. nucleatum was enriched in CRC tissue, but whether that is a cause of the pathology or a consequence, has not yet been clearly defined.

KW - Colorectal cancer

KW - Cytokines

KW - Fusobacterium nucleatum

KW - Inflammation

KW - Microbiota

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JO - Revista de Gastroenterologia de Mexico

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Association of Fusobacterium nucleatum infection and colorectal cancer: A Mexican study

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