Association of CYP1A1 and CYP1B1 polymorphisms with bone mineral density variations in postmenopausal Mexican-Mestizo women

Bertha Chávez, Felipe Vilchis, David Rojano-Mejía, Ramón Mauricio Coral Vázquez, María del Carmen Aguirre-García, Patricia Canto

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Abstract

© 2017 Informa UK Limited, trading as Taylor & Francis Group. Herein, we investigated potential associations between polymorphisms of genes related to estrogen metabolism and bone mineral density (BMD) in postmenopausal women. This was a cross-sectional study, in which two hundred and ninety postmenopausal Mexican-Mestizo women were studied. The BMD of the lumbar spine (LS), total hip (TH), and femoral neck (FN) was measured. The distribution of the genetic polymorphisms, including rs1799814 and rs1048943 at CYP1A1 as well as rs1056836 at CYP1B1, were analyzed by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP), single-stranded conformational polymorphism (SSCP), and DNA sequencing. Deviations from Hardy–Weinberg equilibrium (HWE) were tested, and linkage disequilibrium (LD) was calculated by direct correlation (r2). Moreover, haplotype analysis was performed. All polymorphisms were in HWE. The genotype and allele distributions of the three single nucleotide polymorphisms (SNPs) studied showed no significant differences. However, statistical significance was reached when constructing haplotypes. The CG haplotype in CYP1A1 was associated with variations in LS and FN BMD after adjustment for covariates (p = 0.021 and 0.045, respectively), but the association with TH BMD was not significant. These results suggested that the CG haplotype in CYP1A1 may play an important role in the mechanism of osteoporosis and may be useful as a genetic marker.
Original languageAmerican English
Pages (from-to)607-610
Number of pages545
JournalGynecological Endocrinology
DOIs
StatePublished - 3 Aug 2017

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Cytochrome P-450 CYP1A1
Bone Density
Haplotypes
Femur Neck
Spine
Pelvic Bones
Single-Stranded Conformational Polymorphism
Single-Stranded DNA
Linkage Disequilibrium
Genetic Polymorphisms
DNA Sequence Analysis
Genetic Markers
Osteoporosis
Single Nucleotide Polymorphism
Hip
Estrogens
Cross-Sectional Studies
Alleles
Genotype
Genes

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Chávez, Bertha ; Vilchis, Felipe ; Rojano-Mejía, David ; Coral Vázquez, Ramón Mauricio ; Aguirre-García, María del Carmen ; Canto, Patricia. / Association of CYP1A1 and CYP1B1 polymorphisms with bone mineral density variations in postmenopausal Mexican-Mestizo women. In: Gynecological Endocrinology. 2017 ; pp. 607-610.
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Association of CYP1A1 and CYP1B1 polymorphisms with bone mineral density variations in postmenopausal Mexican-Mestizo women. / Chávez, Bertha; Vilchis, Felipe; Rojano-Mejía, David; Coral Vázquez, Ramón Mauricio; Aguirre-García, María del Carmen; Canto, Patricia.

In: Gynecological Endocrinology, 03.08.2017, p. 607-610.

Research output: Contribution to journalArticle

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