Association between promoter hypermethylation of the DACT2 gene and tumor stages in breast cancer

Veronica Marusa Borgonio-Cuadra, Antonio Miranda-Duarte, Xochitl Rojas-Toledo, Normand Garcia-Hernandez, Jose Alfredo Sierra-Ramirez, Maura Cardenas-Garcia, Marta Elena Hernandez-Caballero

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7 Scopus citations

Abstract

Purpose: Aberrant methylation of CpG islands in the promoter is a hallmark of cancer, leading to transcriptional silencing of tumor suppressor genes. The aim of this work was to evaluate the promoter methylation status of the DACT2 gene in breast cancer (BC) tissue and to analyze its possible effect on tumor type or grade. Methods: CpG island from the DACT2 promoter in region -240 to -14 from transcriptional start site (TSS) were obtained. Through the use of sodium bisulfite DNA conversion analysis, followed by detection with MSP (methylation specific PCR), we analyzed 79 BC and 15 adjacent healthy samples. Results: T he c ases a nalyzed w ere i n s tage I ( 2.5%), I I (38%), or III (59.5%). The most frequent tumor type was invasive ductal carcinoma (71.4%). Methylation analysis comparing tumor tissues with adjacent non-cancerous tissues showed statistical significance. Methylation was observed in 32.9% (26/79) of the samples; no methylation was found in adjacent healthy tissue. DACT2 methylation was associated with tumor stage I-II (p=0.03) and stage III (p= 0.004). Conclusion: An association was found of DACT2 promoter methylation with advanced tumor stages. This gene has been suggested as a potential biomarker, however, more investigation is required to validate this function.

Original languageEnglish
Pages (from-to)361-365
Number of pages5
JournalJournal of B.U.ON.
Volume23
Issue number2
StatePublished - 1 Mar 2018

Keywords

  • Breast cancer
  • CpG island
  • DACT2
  • Methylation
  • Tumor grade

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