TY - JOUR
T1 - Arthrospira maxima paradoxical effect on Trypanosoma cruzi infection
AU - Reboreda-Hernandez, Oscar A.
AU - Juarez-Serrano, Adriana L.
AU - Garcia-Luna, Ivan
AU - Rivero-Ramirez, Nora L.
AU - Ortiz-Butron, Rocio
AU - Nogueda-Torres, Benjamín
AU - Gonzalez-Rodriguez, Nayeli
N1 - Publisher Copyright:
© 2020, Tehran University of Medical Sciences (TUMS). All rights reserved.
PY - 2020
Y1 - 2020
N2 - Background: There are only two anti-trypanocidal drugs available, both have a lot of side effects. This is the pioneer study designed to evaluate the Arthrospira maxima effect in Trypanosoma cruzi-infected mice and macrophages. Methods: A. maxima was administered in vivo, and in vitro (120µL/mL; 200 µL/mL; 500 µL/mL; 852 µL/mL) as prophylaxis, and treatment. In vitro, phagocy-tosis and viability were measured in macrophages cultures supplemented with A. maxima, and T. cruzi-infected. In vivo A. maxima was supplemented to T. cruzi-infected mice in order to obtain the parasitemia curves, parasite amount, and histo-pathologic changes. This assay was performed in Biological Sciences National School of National Polytechnic Institute, Mexico City, in 2019. Results: In vivo, A. maxima administration exacerbates the immune innate host´s response, followed by mice early death. In vitro, A. maxima supplementation promote T. cruzi-macrophage phagocytosis, but also a sooner T. cruzi-infected macro-phage death. Conclusion: A. maxima administration overactive the immune system, decreasing the parasitemia, but causing a severe tissue damage. Then, this nutraceutical has a paradoxical effect on intracellular parasitic infections such as Chagas disease.
AB - Background: There are only two anti-trypanocidal drugs available, both have a lot of side effects. This is the pioneer study designed to evaluate the Arthrospira maxima effect in Trypanosoma cruzi-infected mice and macrophages. Methods: A. maxima was administered in vivo, and in vitro (120µL/mL; 200 µL/mL; 500 µL/mL; 852 µL/mL) as prophylaxis, and treatment. In vitro, phagocy-tosis and viability were measured in macrophages cultures supplemented with A. maxima, and T. cruzi-infected. In vivo A. maxima was supplemented to T. cruzi-infected mice in order to obtain the parasitemia curves, parasite amount, and histo-pathologic changes. This assay was performed in Biological Sciences National School of National Polytechnic Institute, Mexico City, in 2019. Results: In vivo, A. maxima administration exacerbates the immune innate host´s response, followed by mice early death. In vitro, A. maxima supplementation promote T. cruzi-macrophage phagocytosis, but also a sooner T. cruzi-infected macro-phage death. Conclusion: A. maxima administration overactive the immune system, decreasing the parasitemia, but causing a severe tissue damage. Then, this nutraceutical has a paradoxical effect on intracellular parasitic infections such as Chagas disease.
KW - Chagas disease
KW - Dietary supplements
KW - Spirulina
KW - Trypanosoma cruzi
UR - http://www.scopus.com/inward/record.url?scp=85086265610&partnerID=8YFLogxK
M3 - Artículo
AN - SCOPUS:85086265610
SN - 1735-7020
VL - 15
SP - 223
EP - 232
JO - Iranian Journal of Parasitology
JF - Iranian Journal of Parasitology
IS - 2
ER -