Apoptosis de linfocitos en dermatitis atópica en el tratamiento con factor de transferencia

Background: Atopic dermatitis is an inflammatory dysfunction in whose physiopathology the lymphocytes T play an important role in the regulation of the inflammatory process. Objective: To determine and to characterize the role of apoptosis of lymphocytes in patients with moderate to severe atopic dermatitis before and after treatment with transfer factor. Material and methods: Fifteen patients with moderate to severe atopic dermatitis in a range of age from 5 to 45 were included in the studio. Fifteen healthy subjects were taken as a group control. In all subjects it was determined the apoptosis of the lymphocytes by means of anexine and tunnel techniques, as well as the expression of CD95 cells. The 15 patients with atopic dermatitis received treatment with factor transfer in stepped dose as follows: 1 U/day/5 doses, 1 U/week/3 doses, 1 U/15 days/2 doses, 1 U/30 days, until completing three months. At the end of this period new determinations were done to measure apoptosis of lymphocytes and PMN. At the beginning and at the end assessments of the severity in relation to the scale SCORAD were made. Results: By means of both techniques there was not found significant difference in the percentage of apoptotic lymphocytes between patients and control subjects. Differences of the expression of CD95 between patients and control subjects before and after treatment were not significant. There was a significant difference (p < 0.01) of severity from the beginning to the end of treatment with transfer factor in patients with atopic dermatitis. Conclusion: There were found significant differences of apoptosis of lymphocytes in patients with atopic dermatitis who received treatment and control subjects, before neither after the treatment with transfer factor. It was verified the decrease in the severity of the symptoms related to the treatment with transfer factor.