Antiprotozoal constituents from Annona cherimola Miller, a plant used in mexican traditional medicine for the treatment of diarrhea and dysentery

Fernando Calzada, Jose Correa-Basurto, Elizabeth Barbosa, David Mendez-Luna, Lilian Yepez-Mulia

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

© 2017 Pharmacognosy Magazine Published by Wolters Kluwer Medknow. Background:Annona cherimola Miller (Annonaceae) is a medicinal plant frequently recommended in Mexican traditional medicine for the treatment of gastrointestinal disorders such as diarrhea and dysentery.Objective: This work was undertaken to obtain information that support the traditional use of A. cherimola, on pharmacological basis using in vitro and computational experiments.Material and Methods: Bioassay-guided fractionation of the ethanol extract of the leaves of A. cherimola afforded five phenolic compounds:caffeic acid, quercetin, kaempferol, nicotinflorin, and rutin.Results: The in vitro antiprotozoal assay showed that kaempferol was the most potent antiamoebic and antigiardial compound with IC50values of 7.9 μg/mL for Entamoeba histolytica and 8.7 μg/mL for Giardia lamblia. Computational molecular docking study showed that kaempferol interacted in a region different than metronidazole in the enzyme pyruvate:ferredoxin oxidoreductase (PFOR).Conclusion: Considering that PFOR is a target of metronidazole; kaempferol may be a lead compound for the development of novel antiprotozoal agent. Also, these findings give support to the use of A. cherimola in the traditional medicine from México for the treatment of diarrhea and dysentery.
Original languageAmerican English
Pages (from-to)148-152
Number of pages132
JournalPharmacognosy Magazine
DOIs
StatePublished - 1 Jan 2017

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Annona
Dysentery
Traditional Medicine
Diarrhea
Pyruvate Synthase
Metronidazole
Antiprotozoal Agents
Pharmacognosy
Giardia lamblia
Rutin
Entamoeba histolytica
Quercetin
Therapeutics
Medicinal Plants
Biological Assay
Ethanol
Pharmacology
kaempferol
Enzymes

Cite this

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title = "Antiprotozoal constituents from Annona cherimola Miller, a plant used in mexican traditional medicine for the treatment of diarrhea and dysentery",
abstract = "{\circledC} 2017 Pharmacognosy Magazine Published by Wolters Kluwer Medknow. Background:Annona cherimola Miller (Annonaceae) is a medicinal plant frequently recommended in Mexican traditional medicine for the treatment of gastrointestinal disorders such as diarrhea and dysentery.Objective: This work was undertaken to obtain information that support the traditional use of A. cherimola, on pharmacological basis using in vitro and computational experiments.Material and Methods: Bioassay-guided fractionation of the ethanol extract of the leaves of A. cherimola afforded five phenolic compounds:caffeic acid, quercetin, kaempferol, nicotinflorin, and rutin.Results: The in vitro antiprotozoal assay showed that kaempferol was the most potent antiamoebic and antigiardial compound with IC50values of 7.9 μg/mL for Entamoeba histolytica and 8.7 μg/mL for Giardia lamblia. Computational molecular docking study showed that kaempferol interacted in a region different than metronidazole in the enzyme pyruvate:ferredoxin oxidoreductase (PFOR).Conclusion: Considering that PFOR is a target of metronidazole; kaempferol may be a lead compound for the development of novel antiprotozoal agent. Also, these findings give support to the use of A. cherimola in the traditional medicine from M{\'e}xico for the treatment of diarrhea and dysentery.",
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Antiprotozoal constituents from Annona cherimola Miller, a plant used in mexican traditional medicine for the treatment of diarrhea and dysentery. / Calzada, Fernando; Correa-Basurto, Jose; Barbosa, Elizabeth; Mendez-Luna, David; Yepez-Mulia, Lilian.

In: Pharmacognosy Magazine, 01.01.2017, p. 148-152.

Research output: Contribution to journalArticle

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