TY - JOUR
T1 - Antiprotozoal constituents from Annona cherimola Miller, a plant used in mexican traditional medicine for the treatment of diarrhea and dysentery
AU - Calzada, Fernando
AU - Correa-Basurto, Jose
AU - Barbosa, Elizabeth
AU - Mendez-Luna, David
AU - Yepez-Mulia, Lilian
N1 - Publisher Copyright:
© 2017 Pharmacognosy Magazine Published by Wolters Kluwer Medknow.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Background:Annona cherimola Miller (Annonaceae) is a medicinal plant frequently recommended in Mexican traditional medicine for the treatment of gastrointestinal disorders such as diarrhea and dysentery.Objective: This work was undertaken to obtain information that support the traditional use of A. cherimola, on pharmacological basis using in vitro and computational experiments.Material and Methods: Bioassay-guided fractionation of the ethanol extract of the leaves of A. cherimola afforded five phenolic compounds:caffeic acid, quercetin, kaempferol, nicotinflorin, and rutin.Results: The in vitro antiprotozoal assay showed that kaempferol was the most potent antiamoebic and antigiardial compound with IC50 values of 7.9 μg/mL for Entamoeba histolytica and 8.7 μg/mL for Giardia lamblia. Computational molecular docking study showed that kaempferol interacted in a region different than metronidazole in the enzyme pyruvate:ferredoxin oxidoreductase (PFOR).Conclusion: Considering that PFOR is a target of metronidazole; kaempferol may be a lead compound for the development of novel antiprotozoal agent. Also, these findings give support to the use of A. cherimola in the traditional medicine from México for the treatment of diarrhea and dysentery.
AB - Background:Annona cherimola Miller (Annonaceae) is a medicinal plant frequently recommended in Mexican traditional medicine for the treatment of gastrointestinal disorders such as diarrhea and dysentery.Objective: This work was undertaken to obtain information that support the traditional use of A. cherimola, on pharmacological basis using in vitro and computational experiments.Material and Methods: Bioassay-guided fractionation of the ethanol extract of the leaves of A. cherimola afforded five phenolic compounds:caffeic acid, quercetin, kaempferol, nicotinflorin, and rutin.Results: The in vitro antiprotozoal assay showed that kaempferol was the most potent antiamoebic and antigiardial compound with IC50 values of 7.9 μg/mL for Entamoeba histolytica and 8.7 μg/mL for Giardia lamblia. Computational molecular docking study showed that kaempferol interacted in a region different than metronidazole in the enzyme pyruvate:ferredoxin oxidoreductase (PFOR).Conclusion: Considering that PFOR is a target of metronidazole; kaempferol may be a lead compound for the development of novel antiprotozoal agent. Also, these findings give support to the use of A. cherimola in the traditional medicine from México for the treatment of diarrhea and dysentery.
KW - Annona cherimola
KW - Annonaceae
KW - Antiprotozoal activity
KW - Entamoeba histolytica
KW - Giardia lamblia
KW - Piruvate:ferredoxin oxidoreductase
KW - kaempferol
UR - http://www.scopus.com/inward/record.url?scp=85009752563&partnerID=8YFLogxK
U2 - 10.4103/0973-1296.197636
DO - 10.4103/0973-1296.197636
M3 - Artículo
C2 - 28216899
SN - 0973-1296
VL - 13
SP - 148
EP - 152
JO - Pharmacognosy Magazine
JF - Pharmacognosy Magazine
IS - 49
ER -