Antioxidant and antiproliferative activity of the ethanolic extract of equisetum myriochaetum and molecular docking of its main metabolites (Apigenin, kaempferol, and quercetin) on β-tubulin

Fabián Olazarán-Santibañez, Gildardo Rivera, Venancio Vanoye-Eligio, Arturo Mora-Olivo, Gabriel Aguirre-Guzmán, Mónica Ramírez-Cabrera, Eder Arredondo-Espinoza

Research output: Contribution to journalArticlepeer-review

Abstract

Equisetum myriochaetum is a semi-aquatic plant found on riverbanks that is commonly used in traditional medicine as a diuretic agent. Additionally, the genus Equisetum stands out for its content of the flavonoid kaempferol, a well-known antiproliferative agent. Therefore, in this study, E. myriochaetum ethanolic extract was tested in vitro against a cervical cancer cell line (SiHa). Additionally, the antioxidative activity was evaluated through a 2,2-diphenyl-1-picrilhidrazil (DPPH) assay. Finally, a molecular docking analysis of apigenin, kaempferol, and quercetin on the active site of β-tubulin was performed to investigate their potential mechanism of action. All fractions of E. myriochaetum ethanolic extract showed antioxidative activity. Fraction 14 displayed an antiproliferative capacity with a half maximal inhibitory concentration (IC50) value of 6.78 µg/mL against SiHa cells.

Original languageEnglish
Article number443
JournalMolecules
Volume26
Issue number2
DOIs
StatePublished - 1 Jan 2021

Keywords

  • Antioxidative
  • Antiproliferative
  • Equisetum myriochaetum
  • Flavonoids
  • Kaempferol
  • Molecular docking

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