Antioxidant and antiproliferative activity of the ethanolic extract of equisetum myriochaetum and molecular docking of its main metabolites (Apigenin, kaempferol, and quercetin) on β-tubulin

Fabián Olazarán-Santibañez; Gildardo Rivera; Venancio Vanoye-Eligio; Arturo Mora-Olivo; Gabriel Aguirre-Guzmán; Mónica Ramírez-Cabrera; Eder Arredondo-Espinoza

doi:10.3390/molecules26020443

Antioxidant and antiproliferative activity of the ethanolic extract of equisetum myriochaetum and molecular docking of its main metabolites (Apigenin, kaempferol, and quercetin) on β-tubulin

Fabián Olazarán-Santibañez, Gildardo Rivera, Venancio Vanoye-Eligio, Arturo Mora-Olivo, Gabriel Aguirre-Guzmán, Mónica Ramírez-Cabrera, Eder Arredondo-Espinoza

Centro de Biotecnología Genómica (CBG)

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Equisetum myriochaetum is a semi-aquatic plant found on riverbanks that is commonly used in traditional medicine as a diuretic agent. Additionally, the genus Equisetum stands out for its content of the flavonoid kaempferol, a well-known antiproliferative agent. Therefore, in this study, E. myriochaetum ethanolic extract was tested in vitro against a cervical cancer cell line (SiHa). Additionally, the antioxidative activity was evaluated through a 2,2-diphenyl-1-picrilhidrazil (DPPH) assay. Finally, a molecular docking analysis of apigenin, kaempferol, and quercetin on the active site of β-tubulin was performed to investigate their potential mechanism of action. All fractions of E. myriochaetum ethanolic extract showed antioxidative activity. Fraction 14 displayed an antiproliferative capacity with a half maximal inhibitory concentration (IC₅₀) value of 6.78 µg/mL against SiHa cells.

Original language	English
Article number	443
Journal	Molecules
Volume	26
Issue number	2
DOIs	https://doi.org/10.3390/molecules26020443
State	Published - 1 Jan 2021

Keywords

Antioxidative
Antiproliferative
Equisetum myriochaetum
Flavonoids
Kaempferol
Molecular docking

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/molecules26020443

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Olazarán-Santibañez, F., Rivera, G., Vanoye-Eligio, V., Mora-Olivo, A., Aguirre-Guzmán, G., Ramírez-Cabrera, M., & Arredondo-Espinoza, E. (2021). Antioxidant and antiproliferative activity of the ethanolic extract of equisetum myriochaetum and molecular docking of its main metabolites (Apigenin, kaempferol, and quercetin) on β-tubulin. Molecules, 26(2), Article 443. https://doi.org/10.3390/molecules26020443

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title = "Antioxidant and antiproliferative activity of the ethanolic extract of equisetum myriochaetum and molecular docking of its main metabolites (Apigenin, kaempferol, and quercetin) on β-tubulin",

abstract = "Equisetum myriochaetum is a semi-aquatic plant found on riverbanks that is commonly used in traditional medicine as a diuretic agent. Additionally, the genus Equisetum stands out for its content of the flavonoid kaempferol, a well-known antiproliferative agent. Therefore, in this study, E. myriochaetum ethanolic extract was tested in vitro against a cervical cancer cell line (SiHa). Additionally, the antioxidative activity was evaluated through a 2,2-diphenyl-1-picrilhidrazil (DPPH) assay. Finally, a molecular docking analysis of apigenin, kaempferol, and quercetin on the active site of β-tubulin was performed to investigate their potential mechanism of action. All fractions of E. myriochaetum ethanolic extract showed antioxidative activity. Fraction 14 displayed an antiproliferative capacity with a half maximal inhibitory concentration (IC50) value of 6.78 µg/mL against SiHa cells.",

keywords = "Antioxidative, Antiproliferative, Equisetum myriochaetum, Flavonoids, Kaempferol, Molecular docking",

author = "Fabi{\'a}n Olazar{\'a}n-Santiba{\~n}ez and Gildardo Rivera and Venancio Vanoye-Eligio and Arturo Mora-Olivo and Gabriel Aguirre-Guzm{\'a}n and M{\'o}nica Ram{\'i}rez-Cabrera and Eder Arredondo-Espinoza",

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year = "2021",

month = jan,

day = "1",

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Olazarán-Santibañez, F, Rivera, G, Vanoye-Eligio, V, Mora-Olivo, A, Aguirre-Guzmán, G, Ramírez-Cabrera, M & Arredondo-Espinoza, E 2021, 'Antioxidant and antiproliferative activity of the ethanolic extract of equisetum myriochaetum and molecular docking of its main metabolites (Apigenin, kaempferol, and quercetin) on β-tubulin', Molecules, vol. 26, no. 2, 443. https://doi.org/10.3390/molecules26020443

Antioxidant and antiproliferative activity of the ethanolic extract of equisetum myriochaetum and molecular docking of its main metabolites (Apigenin, kaempferol, and quercetin) on β-tubulin. / Olazarán-Santibañez, Fabián; Rivera, Gildardo; Vanoye-Eligio, Venancio et al.
In: Molecules, Vol. 26, No. 2, 443, 01.01.2021.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Antioxidant and antiproliferative activity of the ethanolic extract of equisetum myriochaetum and molecular docking of its main metabolites (Apigenin, kaempferol, and quercetin) on β-tubulin

AU - Olazarán-Santibañez, Fabián

AU - Rivera, Gildardo

AU - Vanoye-Eligio, Venancio

AU - Mora-Olivo, Arturo

AU - Aguirre-Guzmán, Gabriel

AU - Ramírez-Cabrera, Mónica

AU - Arredondo-Espinoza, Eder

PY - 2021/1/1

Y1 - 2021/1/1

N2 - Equisetum myriochaetum is a semi-aquatic plant found on riverbanks that is commonly used in traditional medicine as a diuretic agent. Additionally, the genus Equisetum stands out for its content of the flavonoid kaempferol, a well-known antiproliferative agent. Therefore, in this study, E. myriochaetum ethanolic extract was tested in vitro against a cervical cancer cell line (SiHa). Additionally, the antioxidative activity was evaluated through a 2,2-diphenyl-1-picrilhidrazil (DPPH) assay. Finally, a molecular docking analysis of apigenin, kaempferol, and quercetin on the active site of β-tubulin was performed to investigate their potential mechanism of action. All fractions of E. myriochaetum ethanolic extract showed antioxidative activity. Fraction 14 displayed an antiproliferative capacity with a half maximal inhibitory concentration (IC50) value of 6.78 µg/mL against SiHa cells.

AB - Equisetum myriochaetum is a semi-aquatic plant found on riverbanks that is commonly used in traditional medicine as a diuretic agent. Additionally, the genus Equisetum stands out for its content of the flavonoid kaempferol, a well-known antiproliferative agent. Therefore, in this study, E. myriochaetum ethanolic extract was tested in vitro against a cervical cancer cell line (SiHa). Additionally, the antioxidative activity was evaluated through a 2,2-diphenyl-1-picrilhidrazil (DPPH) assay. Finally, a molecular docking analysis of apigenin, kaempferol, and quercetin on the active site of β-tubulin was performed to investigate their potential mechanism of action. All fractions of E. myriochaetum ethanolic extract showed antioxidative activity. Fraction 14 displayed an antiproliferative capacity with a half maximal inhibitory concentration (IC50) value of 6.78 µg/mL against SiHa cells.

KW - Antioxidative

KW - Antiproliferative

KW - Equisetum myriochaetum

KW - Flavonoids

KW - Kaempferol

KW - Molecular docking

UR - http://www.scopus.com/inward/record.url?scp=85100327687&partnerID=8YFLogxK

U2 - 10.3390/molecules26020443

DO - 10.3390/molecules26020443

M3 - Artículo

C2 - 33466999

AN - SCOPUS:85100327687

SN - 1420-3049

VL - 26

JO - Molecules

JF - Molecules

IS - 2

M1 - 443

ER -

Antioxidant and antiproliferative activity of the ethanolic extract of equisetum myriochaetum and molecular docking of its main metabolites (Apigenin, kaempferol, and quercetin) on β-tubulin

Abstract

Keywords

UN SDGs

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