Antioxidant and anti‐inflammatory properties of probiotic candidate strains isolated during fermentation of agave (Agave angustifolia haw)

Natalia C. Hernández‐delgado, Edgar Torres‐maravilla, Lino Mayorga‐reyes, Rebeca Martín, Philippe Langella, Ricardo Pérez‐pastén‐borja, María E. Sánchez‐pardo, Luis G. Bermúdez‐humarán

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Agave species are a source of diverse products for human use, such as food, fiber, and beverages, which include mezcal, a distilled beverage produced by spontaneous fermentation. Agave is an excellent source of high amounts of sugars, minerals, and phenolic compounds, which favor the growth of lactic acid bacteria (LAB) and yeast communities. In this work, 20 promising LAB strains with probiotic characteristics were isolated from the agave fermentation stage in mezcal production. The strains belonged to Lactobacillus plantarum (15), Lactobacillus rhamnosus (2), Entero-coccus faecium (2), and Lactococcus lactis (1). These isolates were characterized for their resistance under gastrointestinal conditions, such as lysozyme, acid pH, and bile salts. In addition, the adher-ence of these LABs to human intestinal epithelial cells (Caco‐2 and HT‐29 cells) was tested in vitro and their antioxidant and immunomodulatory profile was determined using cellular models. Lac-tobacillus rhamnosus LM07 and Lactobacillus plantarum LM17 and LM19 strains were selected for their antioxidant properties, and their capacities in an oxidative stress model in intestinal epithelial cells IECs (Caco‐2 and HT‐29 cells) in the presence of hydrogen peroxide were evaluated. Interestingly, Lactobacillus rhamnosus LM07 and Lactobacillus plantarum LM17 and LM19 strains showed anti‐in-flammatory properties in TNF‐α‐stimulated HT‐29 cells. Subsequently, bacterial strains exhibiting antioxidant and anti‐inflammatory properties were tested in vivo in a mouse model with dinitro-benzene sulfonic acid (DNBS)‐induced chronic colitis. Weight loss, intestinal permeability, and cy-tokine profiles were measured in mice as indicators of inflammation. One of the selected strains, Lactobacillus plantarum LM17, improved the health of the mice, as observed by reduced weight loss, and significantly decreased intestinal permeability. Altogether, our results demonstrate the potential of LAB (and lactobacilli in particular) isolated from the agave fermentation stage in mezcal pro-duction. Lactobacillus rhamnosus LM07 and Lactobacillus plantarum LM17 strains represent potential candidates for developing new probiotic supplements to treat inflammatory bowel disease (IBD).

Original languageEnglish
Article number1063
JournalMicroorganisms
Volume9
Issue number5
DOIs
StatePublished - May 2021

Keywords

  • Agave
  • Antioxidant
  • IBD
  • Immunomodulation
  • Lactobacillus
  • Probiotics

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