Antioxidant Activity of Collagen Extracts Obtained from the Skin and Gills of Oreochromis sp.

José Roberto Medina Medrano, Tannia Alexandra Quiñones-Muñoz, Abel Arce-Ortíz, Juan Gabriel Torruco-Uco, Ricardo Hernández-Martínez, Manuel Alejandro Lizardi-Jiménez, Elizabeth Varela-Santos

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Efforts aimed at reduction of fishing waste generated during the evisceration and filleting are scarce. The fishing waste is used in the production of low value-added products, such as flours or silages. It is important to visualize an alternative and profitable use of this waste, as it constitutes a serious environmental problem. This research determined the antioxidant properties of collagenous extracts of skin and galls of Oreochromis sp. The raw materials were characterized by proximal chemical analysis. Three treatments were applied to extract the collagen: salt-soluble collagen, acid-soluble collagen (ASC), and pepsin-hydrolyzed collagen (PHC). The collagenous fractions were hydrolyzed (0.1% pepsin). The recovered collagen yield and antioxidant activity were determined to hydrolyzed collagen (HC) and nonhydrolyzed collagen (NHC). The ASC skin showed the highest extraction yield (3.02%). For galls, only the PHC extraction was feasible (0.16%). Antioxidant analysis of NHC did not reveal radical scavenging activities. HC displayed a 2,2-diphenyl-1-picrylhydrazyl %RSA of 22.58 (ASC skin) and 10.34% (PHC galls), and a 2,2′-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid] %RSA of 30.40% (PHC skin) and 29.43% (PHC galls), respectively. The ASC skin and PHC gall extracts exhibited 94.40% and 81.54% in ferric-reducing antioxidant power, and 43.63 and 38.08 μg ascorbic acid equivalents per milli liter for total antioxidant capacity, respectively. The collagen extracts showed %RSA and chelation of pro-oxidant metal ions. Different mechanism of antioxidant action was identified for the extracts: radical scavengers for HC and metal ion chelators for NHC. In conclusion, red tilapia skin collagen is recommended as an active ingredient of nutraceuticals, pharmaceuticals, or functional foods, for the identified bioactive properties.
Original languageAmerican English
Pages (from-to)1-7
Number of pages7
JournalJournal of Medicinal Food
DOIs
StatePublished - 22 Mar 2019

Keywords

    Cite this

    Medina Medrano, J. R., Quiñones-Muñoz, T. A., Arce-Ortíz, A., Torruco-Uco, J. G., Hernández-Martínez, R., Lizardi-Jiménez, M. A., & Varela-Santos, E. (2019). Antioxidant Activity of Collagen Extracts Obtained from the Skin and Gills of Oreochromis sp. Journal of Medicinal Food, 1-7. https://doi.org/10.1089/jmf.2019.0013
    Medina Medrano, José Roberto ; Quiñones-Muñoz, Tannia Alexandra ; Arce-Ortíz, Abel ; Torruco-Uco, Juan Gabriel ; Hernández-Martínez, Ricardo ; Lizardi-Jiménez, Manuel Alejandro ; Varela-Santos, Elizabeth. / Antioxidant Activity of Collagen Extracts Obtained from the Skin and Gills of Oreochromis sp. In: Journal of Medicinal Food. 2019 ; pp. 1-7.
    @article{022c426701e54a9197a8862445511f73,
    title = "Antioxidant Activity of Collagen Extracts Obtained from the Skin and Gills of Oreochromis sp.",
    abstract = "Efforts aimed at reduction of fishing waste generated during the evisceration and filleting are scarce. The fishing waste is used in the production of low value-added products, such as flours or silages. It is important to visualize an alternative and profitable use of this waste, as it constitutes a serious environmental problem. This research determined the antioxidant properties of collagenous extracts of skin and galls of Oreochromis sp. The raw materials were characterized by proximal chemical analysis. Three treatments were applied to extract the collagen: salt-soluble collagen, acid-soluble collagen (ASC), and pepsin-hydrolyzed collagen (PHC). The collagenous fractions were hydrolyzed (0.1{\%} pepsin). The recovered collagen yield and antioxidant activity were determined to hydrolyzed collagen (HC) and nonhydrolyzed collagen (NHC). The ASC skin showed the highest extraction yield (3.02{\%}). For galls, only the PHC extraction was feasible (0.16{\%}). Antioxidant analysis of NHC did not reveal radical scavenging activities. HC displayed a 2,2-diphenyl-1-picrylhydrazyl {\%}RSA of 22.58 (ASC skin) and 10.34{\%} (PHC galls), and a 2,2′-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid] {\%}RSA of 30.40{\%} (PHC skin) and 29.43{\%} (PHC galls), respectively. The ASC skin and PHC gall extracts exhibited 94.40{\%} and 81.54{\%} in ferric-reducing antioxidant power, and 43.63 and 38.08 μg ascorbic acid equivalents per milli liter for total antioxidant capacity, respectively. The collagen extracts showed {\%}RSA and chelation of pro-oxidant metal ions. Different mechanism of antioxidant action was identified for the extracts: radical scavengers for HC and metal ion chelators for NHC. In conclusion, red tilapia skin collagen is recommended as an active ingredient of nutraceuticals, pharmaceuticals, or functional foods, for the identified bioactive properties.",
    keywords = "chelating activity, free radical scavenging, hydrolysis, protein, red tilapia",
    author = "{Medina Medrano}, {Jos{\'e} Roberto} and Qui{\~n}ones-Mu{\~n}oz, {Tannia Alexandra} and Abel Arce-Ort{\'i}z and Torruco-Uco, {Juan Gabriel} and Ricardo Hern{\'a}ndez-Mart{\'i}nez and Lizardi-Jim{\'e}nez, {Manuel Alejandro} and Elizabeth Varela-Santos",
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    month = "3",
    day = "22",
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    Medina Medrano, JR, Quiñones-Muñoz, TA, Arce-Ortíz, A, Torruco-Uco, JG, Hernández-Martínez, R, Lizardi-Jiménez, MA & Varela-Santos, E 2019, 'Antioxidant Activity of Collagen Extracts Obtained from the Skin and Gills of Oreochromis sp.' Journal of Medicinal Food, pp. 1-7. https://doi.org/10.1089/jmf.2019.0013

    Antioxidant Activity of Collagen Extracts Obtained from the Skin and Gills of Oreochromis sp. / Medina Medrano, José Roberto; Quiñones-Muñoz, Tannia Alexandra ; Arce-Ortíz, Abel ; Torruco-Uco, Juan Gabriel ; Hernández-Martínez, Ricardo ; Lizardi-Jiménez, Manuel Alejandro; Varela-Santos, Elizabeth.

    In: Journal of Medicinal Food, 22.03.2019, p. 1-7.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Antioxidant Activity of Collagen Extracts Obtained from the Skin and Gills of Oreochromis sp.

    AU - Medina Medrano, José Roberto

    AU - Quiñones-Muñoz, Tannia Alexandra

    AU - Arce-Ortíz, Abel

    AU - Torruco-Uco, Juan Gabriel

    AU - Hernández-Martínez, Ricardo

    AU - Lizardi-Jiménez, Manuel Alejandro

    AU - Varela-Santos, Elizabeth

    PY - 2019/3/22

    Y1 - 2019/3/22

    N2 - Efforts aimed at reduction of fishing waste generated during the evisceration and filleting are scarce. The fishing waste is used in the production of low value-added products, such as flours or silages. It is important to visualize an alternative and profitable use of this waste, as it constitutes a serious environmental problem. This research determined the antioxidant properties of collagenous extracts of skin and galls of Oreochromis sp. The raw materials were characterized by proximal chemical analysis. Three treatments were applied to extract the collagen: salt-soluble collagen, acid-soluble collagen (ASC), and pepsin-hydrolyzed collagen (PHC). The collagenous fractions were hydrolyzed (0.1% pepsin). The recovered collagen yield and antioxidant activity were determined to hydrolyzed collagen (HC) and nonhydrolyzed collagen (NHC). The ASC skin showed the highest extraction yield (3.02%). For galls, only the PHC extraction was feasible (0.16%). Antioxidant analysis of NHC did not reveal radical scavenging activities. HC displayed a 2,2-diphenyl-1-picrylhydrazyl %RSA of 22.58 (ASC skin) and 10.34% (PHC galls), and a 2,2′-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid] %RSA of 30.40% (PHC skin) and 29.43% (PHC galls), respectively. The ASC skin and PHC gall extracts exhibited 94.40% and 81.54% in ferric-reducing antioxidant power, and 43.63 and 38.08 μg ascorbic acid equivalents per milli liter for total antioxidant capacity, respectively. The collagen extracts showed %RSA and chelation of pro-oxidant metal ions. Different mechanism of antioxidant action was identified for the extracts: radical scavengers for HC and metal ion chelators for NHC. In conclusion, red tilapia skin collagen is recommended as an active ingredient of nutraceuticals, pharmaceuticals, or functional foods, for the identified bioactive properties.

    AB - Efforts aimed at reduction of fishing waste generated during the evisceration and filleting are scarce. The fishing waste is used in the production of low value-added products, such as flours or silages. It is important to visualize an alternative and profitable use of this waste, as it constitutes a serious environmental problem. This research determined the antioxidant properties of collagenous extracts of skin and galls of Oreochromis sp. The raw materials were characterized by proximal chemical analysis. Three treatments were applied to extract the collagen: salt-soluble collagen, acid-soluble collagen (ASC), and pepsin-hydrolyzed collagen (PHC). The collagenous fractions were hydrolyzed (0.1% pepsin). The recovered collagen yield and antioxidant activity were determined to hydrolyzed collagen (HC) and nonhydrolyzed collagen (NHC). The ASC skin showed the highest extraction yield (3.02%). For galls, only the PHC extraction was feasible (0.16%). Antioxidant analysis of NHC did not reveal radical scavenging activities. HC displayed a 2,2-diphenyl-1-picrylhydrazyl %RSA of 22.58 (ASC skin) and 10.34% (PHC galls), and a 2,2′-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid] %RSA of 30.40% (PHC skin) and 29.43% (PHC galls), respectively. The ASC skin and PHC gall extracts exhibited 94.40% and 81.54% in ferric-reducing antioxidant power, and 43.63 and 38.08 μg ascorbic acid equivalents per milli liter for total antioxidant capacity, respectively. The collagen extracts showed %RSA and chelation of pro-oxidant metal ions. Different mechanism of antioxidant action was identified for the extracts: radical scavengers for HC and metal ion chelators for NHC. In conclusion, red tilapia skin collagen is recommended as an active ingredient of nutraceuticals, pharmaceuticals, or functional foods, for the identified bioactive properties.

    KW - chelating activity

    KW - free radical scavenging

    KW - hydrolysis

    KW - protein

    KW - red tilapia

    U2 - 10.1089/jmf.2019.0013

    DO - 10.1089/jmf.2019.0013

    M3 - Article

    SP - 1

    EP - 7

    JO - Journal of Medicinal Food

    JF - Journal of Medicinal Food

    SN - 1096-620X

    ER -

    Medina Medrano JR, Quiñones-Muñoz TA, Arce-Ortíz A, Torruco-Uco JG, Hernández-Martínez R, Lizardi-Jiménez MA et al. Antioxidant Activity of Collagen Extracts Obtained from the Skin and Gills of Oreochromis sp. Journal of Medicinal Food. 2019 Mar 22;1-7. https://doi.org/10.1089/jmf.2019.0013