TY - JOUR
T1 - Antinociceptive effect of heliopsis longipes extract and affinin in mice
AU - Déciga-Campos, Myrna
AU - Rios, María Yolanda
AU - Aguilar-Guadarrama, A. Berenice
PY - 2010
Y1 - 2010
N2 - Heliopsis longipes is used as analgesic in Mexican traditional medicine. The present study assesses the possible antinociceptive effect of Heliopsis longipes and describes the pharmacological mechanism of action of the antinociceptive effect of affinin, identified as the one active principle in Heliopsis longipes acetone extract. Intraperitoneal administration of H. longipes extract and affinin produced a dose-dependent antinociceptive effect when assessed in mice submitted to acetic acid and capsaicin tests. Affinin-induced antinociception (30mg/kg, i.p.) was blocked by naltrexone (1mg/kg, s.c.), p-chlorophenylalanine (80mg/kg, i.p.) and flumazenil (5mg/kg, s.c.) suggesting that its pharmacological effect could be due to the activation of opiodergic, serotoninergic and GABAergic systems. In addition, the antinociceptive effect of affinin was attenuated by pretreatment with 1H-[1,2,4]oxadiazolo[1,2-a]quinoxalin-1-one (1mg/kg, s.c.) and glibenclamide (10mg/kg, s.c.) suggesting that the nitric oxide-K+ channels pathway could be involved in its mechanism of action. These results suggest that affinin itself or its derivatives may have potential antinociceptive effects.
AB - Heliopsis longipes is used as analgesic in Mexican traditional medicine. The present study assesses the possible antinociceptive effect of Heliopsis longipes and describes the pharmacological mechanism of action of the antinociceptive effect of affinin, identified as the one active principle in Heliopsis longipes acetone extract. Intraperitoneal administration of H. longipes extract and affinin produced a dose-dependent antinociceptive effect when assessed in mice submitted to acetic acid and capsaicin tests. Affinin-induced antinociception (30mg/kg, i.p.) was blocked by naltrexone (1mg/kg, s.c.), p-chlorophenylalanine (80mg/kg, i.p.) and flumazenil (5mg/kg, s.c.) suggesting that its pharmacological effect could be due to the activation of opiodergic, serotoninergic and GABAergic systems. In addition, the antinociceptive effect of affinin was attenuated by pretreatment with 1H-[1,2,4]oxadiazolo[1,2-a]quinoxalin-1-one (1mg/kg, s.c.) and glibenclamide (10mg/kg, s.c.) suggesting that the nitric oxide-K+ channels pathway could be involved in its mechanism of action. These results suggest that affinin itself or its derivatives may have potential antinociceptive effects.
KW - Affinin
KW - Antinociceptive activity
KW - Capsaicin test
KW - Compositae
KW - Heliopsis longipes
KW - Writhing test
UR - http://www.scopus.com/inward/record.url?scp=77952043296&partnerID=8YFLogxK
U2 - 10.1055/s-0029-1240658
DO - 10.1055/s-0029-1240658
M3 - Artículo
C2 - 19950048
SN - 0032-0943
VL - 76
SP - 665
EP - 670
JO - Planta Medica
JF - Planta Medica
IS - 7
ER -