TY - JOUR
T1 - Antinociceptive and anti-inflammatory effects of compounds isolated from Scaphyglottis livida and Maxillaria densa
AU - Déciga-Campos, Myrna
AU - Palacios-Espinosa, Juan Francisco
AU - Reyes-Ramírez, Adelfo
AU - Mata, Rachel
N1 - Funding Information:
This research was supported by a CONACYT grant (45814-Q) and DGAPA (IN212005). We are grateful to Dr. Jorge E. Torres-López (UJAT) for the hot-plate equipment. This work is part of the Master of Science thesis of Juan F. Palacios-Espinosa. We are grateful to Isabel Rivero and Laura Acevedo for technical assistance.
PY - 2007/11/1
Y1 - 2007/11/1
N2 - Oral administration of a CH2Cl2-MeOH (1:1) extract of Scaphyglottis livida produced dose-dependent antinociceptive and anti-inflammatory effects when tested in mice and rats using the hot-plate (150-600 mg/kg) and carrageenan-induced inflammation (150-600 mg/kg) models, respectively. Morphine (1.5-6 mg/kg, p.o.) and indomethacin (10-40 mg/kg, p.o.) were used as positive controls, respectively. Four compounds were isolated from the active extract of Scaphyglottis livida, namely 5α-lanosta-24,24-dimethyl-9(11),25-dien-3β-ol (LDD), 24,24,dimethyl-9,19-cyclolanosta-9(11),25-dien-3-one (cyclobalanone), gigantol and 3,4′-dihydroxy-3′,4,5-trimethoxybibenzyl (DTB). LDD and gigantol (25-100 mg/kg, p.o.) significantly increased the hot-plate latency in comparison to vehicle-treated mice and decreased carrageenan-induced inflammation in rats. The antinociception provoked by LDD and gigantol was partially blocked by naloxone (1 mg/kg, i.p.). However, pretreatment with l-NAME (100 mg/kg, i.p.) and glibenclamide (10 mg/kg, i.p.) did not affect the antinociceptive response induced by LDD or gigantol suggesting that their pharmacological effect could be partially due to activation of opioid receptors. Moreover, a CH2Cl2-MeOH (1:1) extract of Maxillaria densa reduced acetic acid-induced abdominal writhes but was not able to produce antinociception in the hot-plate assay. Two compounds were isolated from the active extract of Maxillaria densa, namely fimbriol A and erianthridin. Both compounds partially reduced acetic acid-induced writhes. The results tend to support the popular use of this species in folk medicine for treatment of painful complaints.
AB - Oral administration of a CH2Cl2-MeOH (1:1) extract of Scaphyglottis livida produced dose-dependent antinociceptive and anti-inflammatory effects when tested in mice and rats using the hot-plate (150-600 mg/kg) and carrageenan-induced inflammation (150-600 mg/kg) models, respectively. Morphine (1.5-6 mg/kg, p.o.) and indomethacin (10-40 mg/kg, p.o.) were used as positive controls, respectively. Four compounds were isolated from the active extract of Scaphyglottis livida, namely 5α-lanosta-24,24-dimethyl-9(11),25-dien-3β-ol (LDD), 24,24,dimethyl-9,19-cyclolanosta-9(11),25-dien-3-one (cyclobalanone), gigantol and 3,4′-dihydroxy-3′,4,5-trimethoxybibenzyl (DTB). LDD and gigantol (25-100 mg/kg, p.o.) significantly increased the hot-plate latency in comparison to vehicle-treated mice and decreased carrageenan-induced inflammation in rats. The antinociception provoked by LDD and gigantol was partially blocked by naloxone (1 mg/kg, i.p.). However, pretreatment with l-NAME (100 mg/kg, i.p.) and glibenclamide (10 mg/kg, i.p.) did not affect the antinociceptive response induced by LDD or gigantol suggesting that their pharmacological effect could be partially due to activation of opioid receptors. Moreover, a CH2Cl2-MeOH (1:1) extract of Maxillaria densa reduced acetic acid-induced abdominal writhes but was not able to produce antinociception in the hot-plate assay. Two compounds were isolated from the active extract of Maxillaria densa, namely fimbriol A and erianthridin. Both compounds partially reduced acetic acid-induced writhes. The results tend to support the popular use of this species in folk medicine for treatment of painful complaints.
KW - Anti-inflammatory
KW - Antinociception
KW - Bibenzyls
KW - Maxillaria densa
KW - Orchidaceae
KW - Phenanthrenes
KW - Scaphyglottis livida
KW - Triterpenoids
UR - http://www.scopus.com/inward/record.url?scp=35148827416&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2007.07.021
DO - 10.1016/j.jep.2007.07.021
M3 - Artículo
C2 - 17855030
SN - 0378-8741
VL - 114
SP - 161
EP - 168
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
IS - 2
ER -