TY - JOUR
T1 - Antimicrobial susceptibility pattern of stenotrophomonas species isolated from Mexico
AU - Elufisan, Temidayo O.
AU - Luna, Isabel Cristina Rodriguez
AU - Oyedara, Omotayo O.
AU - Varela, Alejandro Sanchez
AU - García, Virgilio Bocanegra
AU - Oluyide, Busayo O.
AU - Treviño, Samantha Flores
AU - López, Miguel Angel Villalobos
AU - Guo, Xianwu
N1 - Publisher Copyright:
© 2020 Elufisan TO et al. Licensee African Health Sciences.
PY - 2020/4/23
Y1 - 2020/4/23
N2 - Background: Stenotrophomonas species are multi-resistant bacteria with ability to cause opportunistic infections Objective: We isolated 45 Stenotrophomonas species from soil, sewage and the clinic with the aim of investigating their susceptibility to commonly used antimicrobial agents. Methodology: The identities of isolates were confirmed with 16S rRNA gene sequence and MALDI-TOF analysis. Anti-mi-crobial resistance, biofilm production and clonal diversity were also evaluated. The minimum inhibitory concentration technique as described by Clinical & Laboratory Standards Institute: CLSI Guidelines (CLSI) was employed for the evaluation of isolate susceptibility to antibiotics. Result: Forty-five Stenotrophomonas species which include 36 environmental strains and 9 clinical strains of S. maltophilia were considered in this study. 32 (88.9 %) environmental strains were identified to be S. maltophilia, 2 (5.6 %) were Stenotrophomonas nitritireducens, and 2 (5.6 %) cluster as Stenotrophomonas spp. Stenotrophomonas isolates were resistant to at least six of the antibiotics tested, including Trimethoprim/Sulfamethoxazole (SXT). Conclusion: Environmental isolates from this study were resistant to SXT which is commonly used for the treatment of S. maltophilia infections. This informs the need for good public hygiene as the environment could be a reservoir of multi-resistant bacteria. It also buttresses the importance of surveillance study in the management of bacterial resistance.
AB - Background: Stenotrophomonas species are multi-resistant bacteria with ability to cause opportunistic infections Objective: We isolated 45 Stenotrophomonas species from soil, sewage and the clinic with the aim of investigating their susceptibility to commonly used antimicrobial agents. Methodology: The identities of isolates were confirmed with 16S rRNA gene sequence and MALDI-TOF analysis. Anti-mi-crobial resistance, biofilm production and clonal diversity were also evaluated. The minimum inhibitory concentration technique as described by Clinical & Laboratory Standards Institute: CLSI Guidelines (CLSI) was employed for the evaluation of isolate susceptibility to antibiotics. Result: Forty-five Stenotrophomonas species which include 36 environmental strains and 9 clinical strains of S. maltophilia were considered in this study. 32 (88.9 %) environmental strains were identified to be S. maltophilia, 2 (5.6 %) were Stenotrophomonas nitritireducens, and 2 (5.6 %) cluster as Stenotrophomonas spp. Stenotrophomonas isolates were resistant to at least six of the antibiotics tested, including Trimethoprim/Sulfamethoxazole (SXT). Conclusion: Environmental isolates from this study were resistant to SXT which is commonly used for the treatment of S. maltophilia infections. This informs the need for good public hygiene as the environment could be a reservoir of multi-resistant bacteria. It also buttresses the importance of surveillance study in the management of bacterial resistance.
KW - Anti-microbial susceptibility
KW - Biochemical characterization
KW - Clonal diversity
KW - Environmental
KW - Stenotrophomonas
UR - http://www.scopus.com/inward/record.url?scp=85085346272&partnerID=8YFLogxK
U2 - 10.4314/ahs.v20i1.22
DO - 10.4314/ahs.v20i1.22
M3 - Artículo
C2 - 33402905
AN - SCOPUS:85085346272
SN - 1680-6905
VL - 20
SP - 168
EP - 181
JO - African Health Sciences
JF - African Health Sciences
IS - 1
ER -