Antilymphoma potential of the ethanol extract and rutin obtained of the leaves from schinus molle linn.

Fernando Calzada, Jesús Solares-Pascasio, Miguel Valdes, Normand Garcia-Hernandez, Claudia Velázquez, Rosa Ordoñez-Razo, Elizabeth Barbosa

Research output: Contribution to journalArticle

Abstract

© 2018 Pharmacognosy Research | Published by Wolters Kluwer - Medknow. Background: Schinus molle Linn. (Anacardiaceae) is a medicinal plant used by traditional healers in Mexican traditional medicine as antitumoral. Objective: This study was undertaken to obtain information that support the traditional use of the leaves from S. molle as antitumoral. Material and Methods: Antilymphoma properties of the ethanol extract of the leaves from S. molle (EELSm) and rutin were made on athymic CD-1 nu/nu and CD-1 mice inoculated with U-937 cell line (human leukemic monocyte lymphoma [HLML]), and for their antiproliferative effects on U-937 cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Furthermore, the extract and rutin were tested for brine shrimp (BS) toxicity test. In addition, HPLC analysis was realized to known the content of rutin in the leaves from S. molle. Results: An EELSm and rutin exhibited important cytotoxic effects on U-937 cells line (IC50from 172.0 μg/mL and 9.6 μg/mL, respectively) and showed in vivo antitumoral properties on HLML in two murine models (EC50from 52.2 and 9.5 mg/kg to CD-1 nu/nu mice; EC50from 99.4 mg/kg and 6.8 mg/kg to CD-1 mice, respectively). In addition, both showed strong lethality on BS larvae (LC50≤ 22.2 μg/mL). The result of HPLC showed that rutin was the major constituent of EELSm. Conclusions: These test results support traditional medicinal use of S. molle as antitumoral and also suggest that both rutin and EELSm possess antitumor effect on HLML in murine models. Finally, rutin may play an important role in anticancer properties of S. molle. Abbreviations used: EELSm: Ethanol extract of the leaves from S. molle, HLML: Human leukemic monocyte lymphoma, HPLC: High pressure liquid chromatography, BS: Brine shrimp, BSLT: BS lethality test, DMSO: Dimethyl sulfoxide, MTT: 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide.
Original languageAmerican English
Pages (from-to)119-123
Number of pages5
JournalPharmacognosy Research
DOIs
StatePublished - 1 Apr 2018

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Anacardiaceae
Rutin
Artemia
Ethanol
Monocytes
Lymphoma
High Pressure Liquid Chromatography
Dimethyl Sulfoxide
Cell Line
Pharmacognosy
Toxicity Tests
Traditional Medicine
Medicinal Plants
Larva

Cite this

Calzada, F., Solares-Pascasio, J., Valdes, M., Garcia-Hernandez, N., Velázquez, C., Ordoñez-Razo, R., & Barbosa, E. (2018). Antilymphoma potential of the ethanol extract and rutin obtained of the leaves from schinus molle linn. Pharmacognosy Research, 119-123. https://doi.org/10.4103/pr.pr_90_17
Calzada, Fernando ; Solares-Pascasio, Jesús ; Valdes, Miguel ; Garcia-Hernandez, Normand ; Velázquez, Claudia ; Ordoñez-Razo, Rosa ; Barbosa, Elizabeth. / Antilymphoma potential of the ethanol extract and rutin obtained of the leaves from schinus molle linn. In: Pharmacognosy Research. 2018 ; pp. 119-123.
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title = "Antilymphoma potential of the ethanol extract and rutin obtained of the leaves from schinus molle linn.",
abstract = "{\circledC} 2018 Pharmacognosy Research | Published by Wolters Kluwer - Medknow. Background: Schinus molle Linn. (Anacardiaceae) is a medicinal plant used by traditional healers in Mexican traditional medicine as antitumoral. Objective: This study was undertaken to obtain information that support the traditional use of the leaves from S. molle as antitumoral. Material and Methods: Antilymphoma properties of the ethanol extract of the leaves from S. molle (EELSm) and rutin were made on athymic CD-1 nu/nu and CD-1 mice inoculated with U-937 cell line (human leukemic monocyte lymphoma [HLML]), and for their antiproliferative effects on U-937 cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Furthermore, the extract and rutin were tested for brine shrimp (BS) toxicity test. In addition, HPLC analysis was realized to known the content of rutin in the leaves from S. molle. Results: An EELSm and rutin exhibited important cytotoxic effects on U-937 cells line (IC50from 172.0 μg/mL and 9.6 μg/mL, respectively) and showed in vivo antitumoral properties on HLML in two murine models (EC50from 52.2 and 9.5 mg/kg to CD-1 nu/nu mice; EC50from 99.4 mg/kg and 6.8 mg/kg to CD-1 mice, respectively). In addition, both showed strong lethality on BS larvae (LC50≤ 22.2 μg/mL). The result of HPLC showed that rutin was the major constituent of EELSm. Conclusions: These test results support traditional medicinal use of S. molle as antitumoral and also suggest that both rutin and EELSm possess antitumor effect on HLML in murine models. Finally, rutin may play an important role in anticancer properties of S. molle. Abbreviations used: EELSm: Ethanol extract of the leaves from S. molle, HLML: Human leukemic monocyte lymphoma, HPLC: High pressure liquid chromatography, BS: Brine shrimp, BSLT: BS lethality test, DMSO: Dimethyl sulfoxide, MTT: 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide.",
author = "Fernando Calzada and Jes{\'u}s Solares-Pascasio and Miguel Valdes and Normand Garcia-Hernandez and Claudia Vel{\'a}zquez and Rosa Ordo{\~n}ez-Razo and Elizabeth Barbosa",
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Calzada, F, Solares-Pascasio, J, Valdes, M, Garcia-Hernandez, N, Velázquez, C, Ordoñez-Razo, R & Barbosa, E 2018, 'Antilymphoma potential of the ethanol extract and rutin obtained of the leaves from schinus molle linn.', Pharmacognosy Research, pp. 119-123. https://doi.org/10.4103/pr.pr_90_17

Antilymphoma potential of the ethanol extract and rutin obtained of the leaves from schinus molle linn. / Calzada, Fernando; Solares-Pascasio, Jesús; Valdes, Miguel; Garcia-Hernandez, Normand; Velázquez, Claudia; Ordoñez-Razo, Rosa; Barbosa, Elizabeth.

In: Pharmacognosy Research, 01.04.2018, p. 119-123.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antilymphoma potential of the ethanol extract and rutin obtained of the leaves from schinus molle linn.

AU - Calzada, Fernando

AU - Solares-Pascasio, Jesús

AU - Valdes, Miguel

AU - Garcia-Hernandez, Normand

AU - Velázquez, Claudia

AU - Ordoñez-Razo, Rosa

AU - Barbosa, Elizabeth

PY - 2018/4/1

Y1 - 2018/4/1

N2 - © 2018 Pharmacognosy Research | Published by Wolters Kluwer - Medknow. Background: Schinus molle Linn. (Anacardiaceae) is a medicinal plant used by traditional healers in Mexican traditional medicine as antitumoral. Objective: This study was undertaken to obtain information that support the traditional use of the leaves from S. molle as antitumoral. Material and Methods: Antilymphoma properties of the ethanol extract of the leaves from S. molle (EELSm) and rutin were made on athymic CD-1 nu/nu and CD-1 mice inoculated with U-937 cell line (human leukemic monocyte lymphoma [HLML]), and for their antiproliferative effects on U-937 cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Furthermore, the extract and rutin were tested for brine shrimp (BS) toxicity test. In addition, HPLC analysis was realized to known the content of rutin in the leaves from S. molle. Results: An EELSm and rutin exhibited important cytotoxic effects on U-937 cells line (IC50from 172.0 μg/mL and 9.6 μg/mL, respectively) and showed in vivo antitumoral properties on HLML in two murine models (EC50from 52.2 and 9.5 mg/kg to CD-1 nu/nu mice; EC50from 99.4 mg/kg and 6.8 mg/kg to CD-1 mice, respectively). In addition, both showed strong lethality on BS larvae (LC50≤ 22.2 μg/mL). The result of HPLC showed that rutin was the major constituent of EELSm. Conclusions: These test results support traditional medicinal use of S. molle as antitumoral and also suggest that both rutin and EELSm possess antitumor effect on HLML in murine models. Finally, rutin may play an important role in anticancer properties of S. molle. Abbreviations used: EELSm: Ethanol extract of the leaves from S. molle, HLML: Human leukemic monocyte lymphoma, HPLC: High pressure liquid chromatography, BS: Brine shrimp, BSLT: BS lethality test, DMSO: Dimethyl sulfoxide, MTT: 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide.

AB - © 2018 Pharmacognosy Research | Published by Wolters Kluwer - Medknow. Background: Schinus molle Linn. (Anacardiaceae) is a medicinal plant used by traditional healers in Mexican traditional medicine as antitumoral. Objective: This study was undertaken to obtain information that support the traditional use of the leaves from S. molle as antitumoral. Material and Methods: Antilymphoma properties of the ethanol extract of the leaves from S. molle (EELSm) and rutin were made on athymic CD-1 nu/nu and CD-1 mice inoculated with U-937 cell line (human leukemic monocyte lymphoma [HLML]), and for their antiproliferative effects on U-937 cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Furthermore, the extract and rutin were tested for brine shrimp (BS) toxicity test. In addition, HPLC analysis was realized to known the content of rutin in the leaves from S. molle. Results: An EELSm and rutin exhibited important cytotoxic effects on U-937 cells line (IC50from 172.0 μg/mL and 9.6 μg/mL, respectively) and showed in vivo antitumoral properties on HLML in two murine models (EC50from 52.2 and 9.5 mg/kg to CD-1 nu/nu mice; EC50from 99.4 mg/kg and 6.8 mg/kg to CD-1 mice, respectively). In addition, both showed strong lethality on BS larvae (LC50≤ 22.2 μg/mL). The result of HPLC showed that rutin was the major constituent of EELSm. Conclusions: These test results support traditional medicinal use of S. molle as antitumoral and also suggest that both rutin and EELSm possess antitumor effect on HLML in murine models. Finally, rutin may play an important role in anticancer properties of S. molle. Abbreviations used: EELSm: Ethanol extract of the leaves from S. molle, HLML: Human leukemic monocyte lymphoma, HPLC: High pressure liquid chromatography, BS: Brine shrimp, BSLT: BS lethality test, DMSO: Dimethyl sulfoxide, MTT: 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide.

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M3 - Article

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Calzada F, Solares-Pascasio J, Valdes M, Garcia-Hernandez N, Velázquez C, Ordoñez-Razo R et al. Antilymphoma potential of the ethanol extract and rutin obtained of the leaves from schinus molle linn. Pharmacognosy Research. 2018 Apr 1;119-123. https://doi.org/10.4103/pr.pr_90_17