TY - JOUR
T1 - Antibody Response to Nitrogenase-Positive and -Negative Klebsiella pneumoniae Strains in Juvenile-Onset Ankylosing Spondylitis Patients and their First Degree Relatives
T2 - Lack of Differential Recognition of the Bacterial Nitrogenase
AU - Parra-Campos, Víctor
AU - Escobar-Gutiérrez, Alejandro
AU - Domínguez-López, Ma Lilia
AU - Cancino-Díaz, Mario
AU - Burgos-Vargas, Rubén
AU - Granados-Arreola, Julio
AU - Jiménez-Zamudio, Luis
AU - García-Latorre, Ethel
PY - 1996
Y1 - 1996
N2 - In the search for the pathogenic consequences of the molecular mimicry between the Klebsiella pneumoniae nitrogenase and the HLA-B27 antigen, sera from individuals belonging to 16 kindreds with juvenile-onset ankylosing spondylitis cases, were analyzed for antibodies against nitrogenasepositive and -negative K. pneumoniae whole bacterial extracts. An initial screening for nitrogenase producing K. pneumoniae strains was performed in 31 clinical isolates. The best nitrogenase producing strain was selected as well as a non producing one for immunoblot analysis using sera from 82 subjects, 55 HLA-B27 positive, of which 26 had some clinical manifestations. Even though electrophoretic patterns were different in both strains, there was no distinctive differential recognition of the 30-40 kDa proteins where the nitrogenase subcomponent which snares the sequence QTDRED with the HLA-B27 molecule is located. On the other hand, strong recognition of a protein of 60 kDa (p60Kp) was detected in 75% of HLA-B27 positive tested subjects independently of their clinical status. Studies on the nature of this protein and its participation in the pathogenesis of ankylosing spondylitis are now in progress.
AB - In the search for the pathogenic consequences of the molecular mimicry between the Klebsiella pneumoniae nitrogenase and the HLA-B27 antigen, sera from individuals belonging to 16 kindreds with juvenile-onset ankylosing spondylitis cases, were analyzed for antibodies against nitrogenasepositive and -negative K. pneumoniae whole bacterial extracts. An initial screening for nitrogenase producing K. pneumoniae strains was performed in 31 clinical isolates. The best nitrogenase producing strain was selected as well as a non producing one for immunoblot analysis using sera from 82 subjects, 55 HLA-B27 positive, of which 26 had some clinical manifestations. Even though electrophoretic patterns were different in both strains, there was no distinctive differential recognition of the 30-40 kDa proteins where the nitrogenase subcomponent which snares the sequence QTDRED with the HLA-B27 molecule is located. On the other hand, strong recognition of a protein of 60 kDa (p60Kp) was detected in 75% of HLA-B27 positive tested subjects independently of their clinical status. Studies on the nature of this protein and its participation in the pathogenesis of ankylosing spondylitis are now in progress.
UR - http://www.scopus.com/inward/record.url?scp=0030324794&partnerID=8YFLogxK
M3 - Artículo
SN - 0187-4640
VL - 38
SP - 121
EP - 127
JO - Revista latinoamericana de microbiologia
JF - Revista latinoamericana de microbiologia
IS - 2
ER -