Antibody Response to Nitrogenase-Positive and -Negative Klebsiella pneumoniae Strains in Juvenile-Onset Ankylosing Spondylitis Patients and their First Degree Relatives: Lack of Differential Recognition of the Bacterial Nitrogenase

Víctor Parra-Campos, Alejandro Escobar-Gutiérrez, Ma Lilia Domínguez-López, Mario Cancino-Díaz, Rubén Burgos-Vargas, Julio Granados-Arreola, Luis Jiménez-Zamudio, Ethel García-Latorre

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Abstract

In the search for the pathogenic consequences of the molecular mimicry between the Klebsiella pneumoniae nitrogenase and the HLA-B27 antigen, sera from individuals belonging to 16 kindreds with juvenile-onset ankylosing spondylitis cases, were analyzed for antibodies against nitrogenasepositive and -negative K. pneumoniae whole bacterial extracts. An initial screening for nitrogenase producing K. pneumoniae strains was performed in 31 clinical isolates. The best nitrogenase producing strain was selected as well as a non producing one for immunoblot analysis using sera from 82 subjects, 55 HLA-B27 positive, of which 26 had some clinical manifestations. Even though electrophoretic patterns were different in both strains, there was no distinctive differential recognition of the 30-40 kDa proteins where the nitrogenase subcomponent which snares the sequence QTDRED with the HLA-B27 molecule is located. On the other hand, strong recognition of a protein of 60 kDa (p60Kp) was detected in 75% of HLA-B27 positive tested subjects independently of their clinical status. Studies on the nature of this protein and its participation in the pathogenesis of ankylosing spondylitis are now in progress.
Original languageAmerican English
Pages (from-to)121-127
Number of pages108
JournalRevista Latinoamericana de Microbiologia
StatePublished - 1 Dec 1996

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Klebsiella
pneumonia
Nitrogenase
HLA-B27 Antigen
Klebsiella pneumoniae
Ankylosing Spondylitis
nitrogenase
antibodies
Antibodies
Antibody Formation
proteins
Proteins
serums
Palmprint recognition
pathogenesis
antigens
molecular mimicry
Antigens
Molecular Mimicry
Screening

Cite this

@article{04015951cc164a199313f1ed832f58a9,
title = "Antibody Response to Nitrogenase-Positive and -Negative Klebsiella pneumoniae Strains in Juvenile-Onset Ankylosing Spondylitis Patients and their First Degree Relatives: Lack of Differential Recognition of the Bacterial Nitrogenase",
abstract = "In the search for the pathogenic consequences of the molecular mimicry between the Klebsiella pneumoniae nitrogenase and the HLA-B27 antigen, sera from individuals belonging to 16 kindreds with juvenile-onset ankylosing spondylitis cases, were analyzed for antibodies against nitrogenasepositive and -negative K. pneumoniae whole bacterial extracts. An initial screening for nitrogenase producing K. pneumoniae strains was performed in 31 clinical isolates. The best nitrogenase producing strain was selected as well as a non producing one for immunoblot analysis using sera from 82 subjects, 55 HLA-B27 positive, of which 26 had some clinical manifestations. Even though electrophoretic patterns were different in both strains, there was no distinctive differential recognition of the 30-40 kDa proteins where the nitrogenase subcomponent which snares the sequence QTDRED with the HLA-B27 molecule is located. On the other hand, strong recognition of a protein of 60 kDa (p60Kp) was detected in 75{\%} of HLA-B27 positive tested subjects independently of their clinical status. Studies on the nature of this protein and its participation in the pathogenesis of ankylosing spondylitis are now in progress.",
author = "V{\'i}ctor Parra-Campos and Alejandro Escobar-Guti{\'e}rrez and Dom{\'i}nguez-L{\'o}pez, {Ma Lilia} and Mario Cancino-D{\'i}az and Rub{\'e}n Burgos-Vargas and Julio Granados-Arreola and Luis Jim{\'e}nez-Zamudio and Ethel Garc{\'i}a-Latorre",
year = "1996",
month = "12",
day = "1",
language = "American English",
pages = "121--127",
journal = "Revista Latinoamericana de Microbiologia",
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T1 - Antibody Response to Nitrogenase-Positive and -Negative Klebsiella pneumoniae Strains in Juvenile-Onset Ankylosing Spondylitis Patients and their First Degree Relatives: Lack of Differential Recognition of the Bacterial Nitrogenase

AU - Parra-Campos, Víctor

AU - Escobar-Gutiérrez, Alejandro

AU - Domínguez-López, Ma Lilia

AU - Cancino-Díaz, Mario

AU - Burgos-Vargas, Rubén

AU - Granados-Arreola, Julio

AU - Jiménez-Zamudio, Luis

AU - García-Latorre, Ethel

PY - 1996/12/1

Y1 - 1996/12/1

N2 - In the search for the pathogenic consequences of the molecular mimicry between the Klebsiella pneumoniae nitrogenase and the HLA-B27 antigen, sera from individuals belonging to 16 kindreds with juvenile-onset ankylosing spondylitis cases, were analyzed for antibodies against nitrogenasepositive and -negative K. pneumoniae whole bacterial extracts. An initial screening for nitrogenase producing K. pneumoniae strains was performed in 31 clinical isolates. The best nitrogenase producing strain was selected as well as a non producing one for immunoblot analysis using sera from 82 subjects, 55 HLA-B27 positive, of which 26 had some clinical manifestations. Even though electrophoretic patterns were different in both strains, there was no distinctive differential recognition of the 30-40 kDa proteins where the nitrogenase subcomponent which snares the sequence QTDRED with the HLA-B27 molecule is located. On the other hand, strong recognition of a protein of 60 kDa (p60Kp) was detected in 75% of HLA-B27 positive tested subjects independently of their clinical status. Studies on the nature of this protein and its participation in the pathogenesis of ankylosing spondylitis are now in progress.

AB - In the search for the pathogenic consequences of the molecular mimicry between the Klebsiella pneumoniae nitrogenase and the HLA-B27 antigen, sera from individuals belonging to 16 kindreds with juvenile-onset ankylosing spondylitis cases, were analyzed for antibodies against nitrogenasepositive and -negative K. pneumoniae whole bacterial extracts. An initial screening for nitrogenase producing K. pneumoniae strains was performed in 31 clinical isolates. The best nitrogenase producing strain was selected as well as a non producing one for immunoblot analysis using sera from 82 subjects, 55 HLA-B27 positive, of which 26 had some clinical manifestations. Even though electrophoretic patterns were different in both strains, there was no distinctive differential recognition of the 30-40 kDa proteins where the nitrogenase subcomponent which snares the sequence QTDRED with the HLA-B27 molecule is located. On the other hand, strong recognition of a protein of 60 kDa (p60Kp) was detected in 75% of HLA-B27 positive tested subjects independently of their clinical status. Studies on the nature of this protein and its participation in the pathogenesis of ankylosing spondylitis are now in progress.

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