TY - JOUR
T1 - Antibody-coupled hydroxyapatite nanoparticles as efficient tools for labeling intracellular proteins
AU - Vázquez-Hernández, Fabiola
AU - Mendoza-Acevedo, Salvador
AU - Mendoza-Barrera, Claudia Oliva
AU - Mendoza-Álvarez, Julio
AU - Luna-Arias, Juan Pedro
N1 - Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Smart biomaterials for active targeting are a novel way for biosensing, gene and drug delivery, and bioimaging. The functional additives are chosen according to the material carrier characteristics, i.e. the functional mercapto acids of different lengths. In order to identify the target tissue, cell, organ or molecule, the biomaterial must be equipped with a recognizing molecule on its surface. In most cases, semiconductor o metal materials are employed in bioimaging and biosensing applications; in gene and drug delivery area, it is useful to employ porous nanoparticles as carriers. Hydroxyapatite nanoparticles have been proved efficiently in drug delivery. In this work we established a new protocol to obtain smart hydroxyapatite nanoparticles with 3-mercaptopropionic acid and anti-Actin molecules in order to localize actin molecules in cells.
AB - Smart biomaterials for active targeting are a novel way for biosensing, gene and drug delivery, and bioimaging. The functional additives are chosen according to the material carrier characteristics, i.e. the functional mercapto acids of different lengths. In order to identify the target tissue, cell, organ or molecule, the biomaterial must be equipped with a recognizing molecule on its surface. In most cases, semiconductor o metal materials are employed in bioimaging and biosensing applications; in gene and drug delivery area, it is useful to employ porous nanoparticles as carriers. Hydroxyapatite nanoparticles have been proved efficiently in drug delivery. In this work we established a new protocol to obtain smart hydroxyapatite nanoparticles with 3-mercaptopropionic acid and anti-Actin molecules in order to localize actin molecules in cells.
KW - Bioconjugation
KW - Cell viability
KW - Functionalization
KW - Hydroxyapatite
KW - Immunofluorescence
KW - Nanoparticle
UR - http://www.scopus.com/inward/record.url?scp=85005810430&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2016.10.082
DO - 10.1016/j.msec.2016.10.082
M3 - Artículo
C2 - 27987788
SN - 0928-4931
VL - 71
SP - 909
EP - 918
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
ER -