Anti-inflammatory effect of 3-O-[(6'-O-palmitoyl)-β-D-glucopyranosyl sitosterol] from Agave angustifolia on ear edema in mice

Elizabeth Hernández-Valle, Maribel Herrera-Ruiz, Gabriela Rosas Salgado, Alejandro Zamilpa, Martha Lucia Arenas Ocampo, Antonio Jiménez Aparicio, Jaime Tortoriello, Enrique Jiménez-Ferrer

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

In Mexico Agave angustifolia has traditionally been used to treat inflammation. The aim of this study was to measure the anti-inflammatory effect of the extract of A. angustifolia, the isolation and identification of active compounds. From the acetone extract two active fractions were obtained, (AsF13 and AaF16). For the characterization of pharmacological activity, the acute inflammatory model of mouse ear edema induced with TPA was used. The tissue exposed to TPA and treatments were subjected to two analysis, cytokine quantification (IL-1β, IL-6, IL-10 and TNF-α) and histopathological evaluation. The active fraction (AaF16) consisted principally of 3-O-[(6'-O-palmitoyl)-β-D-glucopyranpsyl] sitosterol. In AaF13 fraction was identified β-sitosteryl glucoside (2) and stigmasterol (3). The three treatments tested showed a concentration-dependent anti-inflammatory effect (AaAc Emax = 33.10%, EC50 = 0.126 mg/ear; AaF13 Emax = 54.22%, EC50 = 0.0524 mg/ear; AaF16 Emax = 61.01%, EC50 = 0.050 mg/ear). The application of TPA caused a significant increase on level of IL-1β, IL-6 and TNFα compared with basal condition, which was countered by any of the experimental treatments. Moreover, the experimental treatments induced a significant increase in the levels of IL-4 and IL-10, compared to the level observed when stimulated with TPA. Therefore, the anti-inflammatory effect of Agave angustifolia, is associated with the presence of 3-O-[(6'-O-palmitoyl)-β-D-glucopyranosyl] sitosterol.

Original languageEnglish
Pages (from-to)15624-15637
Number of pages14
JournalMolecules
Volume19
Issue number10
DOIs
StatePublished - 1 Oct 2014

Keywords

  • 3-O-[(6'-O-palmitoyl)-β-D-glucopyranosyl] sitosterol
  • Agave angustifolia
  • Anti-inflammatory

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