@article{c6a090064ad44fa1bee8091682bbc9a8,
title = "Anatomical and histochemical characterization of in vitro haustorium from roots of Castilleja tenuiflora",
abstract = "In vitro induction of haustoria from Castilleja tenuiflora Benth. was achieved by applying 25 μM catechin, 25 μM vanillin, or 25 μM H2O2. Of the treatments tested, 25 μM vanillin was the strongest inducer of haustoria in C. tenuiflora roots in vitro (up to 3 haustoria per root). Haustorium development occurred laterally and was observable 14 d after inducer application. It was characterized by elongation of the epidermal cells and division of the inner cortical cells which also possessed abundant nuclei. Histochemical analysis using 3,3-diaminobenzidine (DAB) and diphenylboric acid 2-aminoethyl ester (DBPA) indicated that the formation of haustoria was associated with the accumulation of H2O2 and flavonoids.",
keywords = "catechin, flavonoids, hemiparasitism, hydrogen peroxide, vanillin",
author = "G. Salcedo-Morales and Jim{\'e}nez-Aparicio, {A. R.} and F. Cruz-Sosa and G. Trejo-Tapia",
note = "Funding Information: Submitted 25 August 2012, last revision 30 April 2013, accepted 2 July 2013. Abbreviations: DAB - 3,3-diaminobenzidine; DBPA - diphenylboric acid 2-aminoethyl ester; DMBQ - 2,6-dimethoxy-p-benzoquinone; EC - epidermal cells; ICC - inner cortical cells; MZ - meristematic zone; SG - starch grain; NZ - zone with nucleus. Acknowledgements: This work was financed by the Secretar{\'i}a de Investigaci{\'o}n y Posgrado del Instituto Polit{\'e}cnico Nacional (SIP-IPN, Grant Nos. 20120745 and 20131786) and the Consejo Nacional de Ciencia y Tecnolog{\'i}a (CONACyT, Grant No. 100202). GSM is indebted to Universidad Aut{\'o}noma Metropolitana-Iztapalapa, CONACyT and IPN (COTEBAL-IPN) for doctoral fellowship awarded. GSM, ARJA and GTT thank EDI and SIBE-IPN. * Corresponding author; fax: (+52) 735 3941896, e-mail: gttapia@ipn.mx; gabriela.trejo@yahoo.com",
year = "2014",
month = mar,
day = "1",
doi = "10.1007/s10535-013-0369-2",
language = "Ingl{\'e}s",
volume = "58",
pages = "164--168",
journal = "Biologia Plantarum",
issn = "0006-3134",
number = "1",
}