Anatomical and histochemical characterization of in vitro haustorium from roots of Castilleja tenuiflora

G. Salcedo-Morales, A. R. Jiménez-Aparicio, F. Cruz-Sosa, G. Trejo-Tapia

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In vitro induction of haustoria from Castilleja tenuiflora Benth. was achieved by applying 25 μM catechin, 25 μM vanillin, or 25 μM H2O2. Of the treatments tested, 25 μM vanillin was the strongest inducer of haustoria in C. tenuiflora roots in vitro (up to 3 haustoria per root). Haustorium development occurred laterally and was observable 14 d after inducer application. It was characterized by elongation of the epidermal cells and division of the inner cortical cells which also possessed abundant nuclei. Histochemical analysis using 3,3-diaminobenzidine (DAB) and diphenylboric acid 2-aminoethyl ester (DBPA) indicated that the formation of haustoria was associated with the accumulation of H2O2 and flavonoids. © 2014 Springer Science+Business Media Dordrecht.
Original languageAmerican English
Pages (from-to)164-168
Number of pages147
JournalBiologia Plantarum
DOIs
StatePublished - 1 Jan 2014

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Orobanchaceae
Castilleja
vanillin
Catechin
catechin
Flavonoids
Cell Division
Esters
flavonoids
esters
cells
Acids
acids
In Vitro Techniques

Cite this

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abstract = "In vitro induction of haustoria from Castilleja tenuiflora Benth. was achieved by applying 25 μM catechin, 25 μM vanillin, or 25 μM H2O2. Of the treatments tested, 25 μM vanillin was the strongest inducer of haustoria in C. tenuiflora roots in vitro (up to 3 haustoria per root). Haustorium development occurred laterally and was observable 14 d after inducer application. It was characterized by elongation of the epidermal cells and division of the inner cortical cells which also possessed abundant nuclei. Histochemical analysis using 3,3-diaminobenzidine (DAB) and diphenylboric acid 2-aminoethyl ester (DBPA) indicated that the formation of haustoria was associated with the accumulation of H2O2 and flavonoids. {\circledC} 2014 Springer Science+Business Media Dordrecht.",
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Anatomical and histochemical characterization of in vitro haustorium from roots of Castilleja tenuiflora. / Salcedo-Morales, G.; Jiménez-Aparicio, A. R.; Cruz-Sosa, F.; Trejo-Tapia, G.

In: Biologia Plantarum, 01.01.2014, p. 164-168.

Research output: Contribution to journalArticle

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AU - Salcedo-Morales, G.

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AU - Trejo-Tapia, G.

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