Analysis of the effect of a 2-(trifluoromethyl)-1H-benzimidazole derivative on Trichinella spiralis muscle larvae

Félix Matadamas-Martínez, Benjamín Nogueda-Torres, Alicia Hernández-Campos, Francisco Hernández-Luis, Rafael Castillo, Guillermo Mendoza, Javier R. Ambrosio, Gabriela Andrés-Antonio, Lilián Yépez-Mulia

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Abstract

Albendazole and mebendazole are widely used in the treatment of trichinellosis; however, chemotherapy failure has been reported. In an effort to develop new anthelminthic compounds, we examined a previously synthesized 2-(trifluoromethyl)-1. H-benzimidazole derivative (1) that showed good in vitro activity against Trichinella spiralis muscle larvae but low in vivo efficacy. In order to improve the solubility of compound 1, an inclusion complex with 2-hydroxypropyl-β-cyclodextrin (1/HP-βCD) was prepared. When 1/HP-βCD was tested in vivo, it significantly reduced the ML burden (84%). In addition, a proteomic analysis of T. spiralis ML treated with 1 revealed significant changes in the expression levels of proteins involved in energy metabolism and the cytoskeleton of the parasite. Compound (1) also induced extensive ultrastructural changes in the cuticle, hypodermis and midgut of the parasite. © 2013 Elsevier B.V.
Original languageAmerican English
Pages (from-to)193-197
Number of pages173
JournalVeterinary Parasitology
DOIs
StatePublished - 25 Feb 2013

Fingerprint

muscle larvae
Trichinella spiralis
benzimidazole
Larva
Parasites
chemical derivatives
Mebendazole
mebendazole
Trichinellosis
parasites
trichinosis
Albendazole
Muscles
albendazole
cyclodextrins
Subcutaneous Tissue
Cyclodextrins
cytoskeleton
protein metabolism
Cytoskeleton

Cite this

Matadamas-Martínez, F., Nogueda-Torres, B., Hernández-Campos, A., Hernández-Luis, F., Castillo, R., Mendoza, G., ... Yépez-Mulia, L. (2013). Analysis of the effect of a 2-(trifluoromethyl)-1H-benzimidazole derivative on Trichinella spiralis muscle larvae. Veterinary Parasitology, 193-197. https://doi.org/10.1016/j.vetpar.2013.01.054
Matadamas-Martínez, Félix ; Nogueda-Torres, Benjamín ; Hernández-Campos, Alicia ; Hernández-Luis, Francisco ; Castillo, Rafael ; Mendoza, Guillermo ; Ambrosio, Javier R. ; Andrés-Antonio, Gabriela ; Yépez-Mulia, Lilián. / Analysis of the effect of a 2-(trifluoromethyl)-1H-benzimidazole derivative on Trichinella spiralis muscle larvae. In: Veterinary Parasitology. 2013 ; pp. 193-197.
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abstract = "Albendazole and mebendazole are widely used in the treatment of trichinellosis; however, chemotherapy failure has been reported. In an effort to develop new anthelminthic compounds, we examined a previously synthesized 2-(trifluoromethyl)-1. H-benzimidazole derivative (1) that showed good in vitro activity against Trichinella spiralis muscle larvae but low in vivo efficacy. In order to improve the solubility of compound 1, an inclusion complex with 2-hydroxypropyl-β-cyclodextrin (1/HP-βCD) was prepared. When 1/HP-βCD was tested in vivo, it significantly reduced the ML burden (84{\%}). In addition, a proteomic analysis of T. spiralis ML treated with 1 revealed significant changes in the expression levels of proteins involved in energy metabolism and the cytoskeleton of the parasite. Compound (1) also induced extensive ultrastructural changes in the cuticle, hypodermis and midgut of the parasite. {\circledC} 2013 Elsevier B.V.",
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Matadamas-Martínez, F, Nogueda-Torres, B, Hernández-Campos, A, Hernández-Luis, F, Castillo, R, Mendoza, G, Ambrosio, JR, Andrés-Antonio, G & Yépez-Mulia, L 2013, 'Analysis of the effect of a 2-(trifluoromethyl)-1H-benzimidazole derivative on Trichinella spiralis muscle larvae', Veterinary Parasitology, pp. 193-197. https://doi.org/10.1016/j.vetpar.2013.01.054

Analysis of the effect of a 2-(trifluoromethyl)-1H-benzimidazole derivative on Trichinella spiralis muscle larvae. / Matadamas-Martínez, Félix; Nogueda-Torres, Benjamín; Hernández-Campos, Alicia; Hernández-Luis, Francisco; Castillo, Rafael; Mendoza, Guillermo; Ambrosio, Javier R.; Andrés-Antonio, Gabriela; Yépez-Mulia, Lilián.

In: Veterinary Parasitology, 25.02.2013, p. 193-197.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Matadamas-Martínez, Félix

AU - Nogueda-Torres, Benjamín

AU - Hernández-Campos, Alicia

AU - Hernández-Luis, Francisco

AU - Castillo, Rafael

AU - Mendoza, Guillermo

AU - Ambrosio, Javier R.

AU - Andrés-Antonio, Gabriela

AU - Yépez-Mulia, Lilián

PY - 2013/2/25

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N2 - Albendazole and mebendazole are widely used in the treatment of trichinellosis; however, chemotherapy failure has been reported. In an effort to develop new anthelminthic compounds, we examined a previously synthesized 2-(trifluoromethyl)-1. H-benzimidazole derivative (1) that showed good in vitro activity against Trichinella spiralis muscle larvae but low in vivo efficacy. In order to improve the solubility of compound 1, an inclusion complex with 2-hydroxypropyl-β-cyclodextrin (1/HP-βCD) was prepared. When 1/HP-βCD was tested in vivo, it significantly reduced the ML burden (84%). In addition, a proteomic analysis of T. spiralis ML treated with 1 revealed significant changes in the expression levels of proteins involved in energy metabolism and the cytoskeleton of the parasite. Compound (1) also induced extensive ultrastructural changes in the cuticle, hypodermis and midgut of the parasite. © 2013 Elsevier B.V.

AB - Albendazole and mebendazole are widely used in the treatment of trichinellosis; however, chemotherapy failure has been reported. In an effort to develop new anthelminthic compounds, we examined a previously synthesized 2-(trifluoromethyl)-1. H-benzimidazole derivative (1) that showed good in vitro activity against Trichinella spiralis muscle larvae but low in vivo efficacy. In order to improve the solubility of compound 1, an inclusion complex with 2-hydroxypropyl-β-cyclodextrin (1/HP-βCD) was prepared. When 1/HP-βCD was tested in vivo, it significantly reduced the ML burden (84%). In addition, a proteomic analysis of T. spiralis ML treated with 1 revealed significant changes in the expression levels of proteins involved in energy metabolism and the cytoskeleton of the parasite. Compound (1) also induced extensive ultrastructural changes in the cuticle, hypodermis and midgut of the parasite. © 2013 Elsevier B.V.

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