© 2015 Elsevier Ltd. All rights reserved. In this work, two electrocatalysts based on Pd (Pd/MWCNT and PdAg/MWCNT) were synthesized using reverse micelles for ethanol oxidation (EOR), and evaluated in an air-breathing microfluidic fuel cell. Commercial Pd (Pd/C) was used for comparison. The average nanoparticle size estimated by TEM was approximately 4-7 nm for both electrocatalysts. TGA measurements indicated that the electrocatalysts had similar metallic loading (19% for Pd and 17% for PdAg). The materials were tested for EOR in 1 M KOH. Fuel cell tests were performed in an air-breathing microfluidic fuel cell (ABμFFC) in which oxygen was obtained from the air to improve the performance of the cathodic reaction. The results were compared with those obtained using a closed microfluidic fuel cell (μFFC). In these devices, two streams were injected, one containing fuel and the other containing an electrolyte solution (1 M KOH), and commercial Pt (Pt/C) was used as cathodic catalyst. The ABμFFC that used the PdAg/MWCNT electrocatalyst performed better (almost 70% more power was harvested) than the one that used the Pd/MWCNT electrocatalyst. Finally, the power density harvested from the ABμFFC was 4 times higher than that harvested from the μFFC.