TY - JOUR
T1 - An alternative model for the transmembrane segments of the yeast H+-ATPase
AU - Pardo, Juan Pablo
AU - Martínez, Federico
AU - Guerra, Guadalupe
AU - Velázquez, Isabel
AU - Rendón, Juan Luis
AU - Mendoza, Guillermo
PY - 1999
Y1 - 1999
N2 - An alternative topological model for the yeast plasma membrane H+-ATPase from K. lactis was deduced by joint prediction, using 11 algorithms for the prediction of transmembrane segments complemented with hydrophobic moment analysis. Similarly to the model currently used in the literature, this alternative model contains 10 transmembrane segments, four in the N-half and six in the C-half of the protein. However, the distribution of the membrane-associated segments on the C-half of the enzyme differs in both models. Nine of the 10 transmembrane segments are highly hydrophobic with low hydrophobic moments, and are probably involved in structural roles. The fifth transmembrane segment is, on the other hand, less hydrophobic, with the highest hydrophobic moment, suggesting that this segment might have a dynamic role in the coupling of the hydrolysis of ATP with the translocation of protons across the membrane. The alignment of the Ca2+-ATPase, the Na+/K+-ATPase and the H+-ATPase sequences showed that these proteins have the same topology in the N-half, but important differences were found at the C-half of the enzymes. In contrast with the mammalian ATPases, the fifth transmembrane segment in the H+-ATPase appears early in the sequence, giving rise to a shorter cytoplasmic central loop. This alternative model will be useful in the designing of site-directed mutagenesis experiments and contains information for the fitting of the amino acid sequence into the transmembrane region of the three-dimensional model of the ATPase.
AB - An alternative topological model for the yeast plasma membrane H+-ATPase from K. lactis was deduced by joint prediction, using 11 algorithms for the prediction of transmembrane segments complemented with hydrophobic moment analysis. Similarly to the model currently used in the literature, this alternative model contains 10 transmembrane segments, four in the N-half and six in the C-half of the protein. However, the distribution of the membrane-associated segments on the C-half of the enzyme differs in both models. Nine of the 10 transmembrane segments are highly hydrophobic with low hydrophobic moments, and are probably involved in structural roles. The fifth transmembrane segment is, on the other hand, less hydrophobic, with the highest hydrophobic moment, suggesting that this segment might have a dynamic role in the coupling of the hydrolysis of ATP with the translocation of protons across the membrane. The alignment of the Ca2+-ATPase, the Na+/K+-ATPase and the H+-ATPase sequences showed that these proteins have the same topology in the N-half, but important differences were found at the C-half of the enzymes. In contrast with the mammalian ATPases, the fifth transmembrane segment in the H+-ATPase appears early in the sequence, giving rise to a shorter cytoplasmic central loop. This alternative model will be useful in the designing of site-directed mutagenesis experiments and contains information for the fitting of the amino acid sequence into the transmembrane region of the three-dimensional model of the ATPase.
KW - H-ATPase
KW - Kluyveromyces lactis
KW - Prediction
KW - Transmembrane segments
UR - http://www.scopus.com/inward/record.url?scp=0344496504&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-0061(199911)15:15<1585::AID-YEA483>3.0.CO;2-A
DO - 10.1002/(SICI)1097-0061(199911)15:15<1585::AID-YEA483>3.0.CO;2-A
M3 - Artículo
SN - 0749-503X
VL - 15
SP - 1585
EP - 1593
JO - Yeast
JF - Yeast
IS - 15
ER -