Amebic liver abscess in rat: Morphological evidence of innate immune modulation by the sympathetic nervous system

Martín Humberto Muñoz-Ortega, Daniel Cervantes-García, Andrés Quintanar-Stephano, María Del Rosario Campos-Esparza, Mario García-Lorenzana, R. Campos-Rodríguez, M. G. Rodríguez, Javier Ventura-Juárez

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Abstract

© 2015, Universidad de la Frontera. All rights reserved. All organs of the immune system are innervated and almost all neurotransmitter receptors are present on immune cells. We studied the effects of sympathetic innervation in the development of amebic liver abscess (ALA) in rats. Our results showed that lack of sympathetic innervation promote a decrease in size of ALA. We found scarce amoebas, increased the number of neutrophils and a few collagen fibers surrounding the abscess, meanwhile in control group, we observed abscesses areas with typical necrosis including trophozoites and neutrophils. Macrophages were differentially distributed surrounding abscess area in control and vehicle groups, but equally located in and outside of the abscesses in sympathectomized rat. No significant differences were observed on NK cells in analysed groups. In cytokines quantification studies, we observed down-expression of IFN-g and TNF-a, moreover, we found overexpression of IL-10 in sympathectomized and ALA group. In conclusion, our results suggest that elimination of sympathetic nerve fibers in a model rat of amebic liver abscess induces reduction of the innate immune response and presence of amebas through the liver at seven days post-inoculation.
Original languageAmerican English
Pages (from-to)213-221
Number of pages9
JournalInternational Journal of Morphology
DOIs
StatePublished - 1 Jan 2015

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Amoebic Liver Abscess
Sympathetic Nervous System
Abscess
Amoeba
Neutrophils
Adrenergic Fibers
Trophozoites
Control Groups
Neurotransmitter Receptor
Nerve Fibers
Innate Immunity
Natural Killer Cells
Interleukin-10
Immune System
Necrosis
Collagen
Macrophages
Cytokines
Liver

Cite this

Muñoz-Ortega, M. H., Cervantes-García, D., Quintanar-Stephano, A., Campos-Esparza, M. D. R., García-Lorenzana, M., Campos-Rodríguez, R., ... Ventura-Juárez, J. (2015). Amebic liver abscess in rat: Morphological evidence of innate immune modulation by the sympathetic nervous system. International Journal of Morphology, 213-221. https://doi.org/10.4067/S0717-95022015000100034
Muñoz-Ortega, Martín Humberto ; Cervantes-García, Daniel ; Quintanar-Stephano, Andrés ; Campos-Esparza, María Del Rosario ; García-Lorenzana, Mario ; Campos-Rodríguez, R. ; Rodríguez, M. G. ; Ventura-Juárez, Javier. / Amebic liver abscess in rat: Morphological evidence of innate immune modulation by the sympathetic nervous system. In: International Journal of Morphology. 2015 ; pp. 213-221.
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abstract = "{\circledC} 2015, Universidad de la Frontera. All rights reserved. All organs of the immune system are innervated and almost all neurotransmitter receptors are present on immune cells. We studied the effects of sympathetic innervation in the development of amebic liver abscess (ALA) in rats. Our results showed that lack of sympathetic innervation promote a decrease in size of ALA. We found scarce amoebas, increased the number of neutrophils and a few collagen fibers surrounding the abscess, meanwhile in control group, we observed abscesses areas with typical necrosis including trophozoites and neutrophils. Macrophages were differentially distributed surrounding abscess area in control and vehicle groups, but equally located in and outside of the abscesses in sympathectomized rat. No significant differences were observed on NK cells in analysed groups. In cytokines quantification studies, we observed down-expression of IFN-g and TNF-a, moreover, we found overexpression of IL-10 in sympathectomized and ALA group. In conclusion, our results suggest that elimination of sympathetic nerve fibers in a model rat of amebic liver abscess induces reduction of the innate immune response and presence of amebas through the liver at seven days post-inoculation.",
author = "Mu{\~n}oz-Ortega, {Mart{\'i}n Humberto} and Daniel Cervantes-Garc{\'i}a and Andr{\'e}s Quintanar-Stephano and Campos-Esparza, {Mar{\'i}a Del Rosario} and Mario Garc{\'i}a-Lorenzana and R. Campos-Rodr{\'i}guez and Rodr{\'i}guez, {M. G.} and Javier Ventura-Ju{\'a}rez",
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Muñoz-Ortega, MH, Cervantes-García, D, Quintanar-Stephano, A, Campos-Esparza, MDR, García-Lorenzana, M, Campos-Rodríguez, R, Rodríguez, MG & Ventura-Juárez, J 2015, 'Amebic liver abscess in rat: Morphological evidence of innate immune modulation by the sympathetic nervous system', International Journal of Morphology, pp. 213-221. https://doi.org/10.4067/S0717-95022015000100034

Amebic liver abscess in rat: Morphological evidence of innate immune modulation by the sympathetic nervous system. / Muñoz-Ortega, Martín Humberto; Cervantes-García, Daniel; Quintanar-Stephano, Andrés; Campos-Esparza, María Del Rosario; García-Lorenzana, Mario; Campos-Rodríguez, R.; Rodríguez, M. G.; Ventura-Juárez, Javier.

In: International Journal of Morphology, 01.01.2015, p. 213-221.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Amebic liver abscess in rat: Morphological evidence of innate immune modulation by the sympathetic nervous system

AU - Muñoz-Ortega, Martín Humberto

AU - Cervantes-García, Daniel

AU - Quintanar-Stephano, Andrés

AU - Campos-Esparza, María Del Rosario

AU - García-Lorenzana, Mario

AU - Campos-Rodríguez, R.

AU - Rodríguez, M. G.

AU - Ventura-Juárez, Javier

PY - 2015/1/1

Y1 - 2015/1/1

N2 - © 2015, Universidad de la Frontera. All rights reserved. All organs of the immune system are innervated and almost all neurotransmitter receptors are present on immune cells. We studied the effects of sympathetic innervation in the development of amebic liver abscess (ALA) in rats. Our results showed that lack of sympathetic innervation promote a decrease in size of ALA. We found scarce amoebas, increased the number of neutrophils and a few collagen fibers surrounding the abscess, meanwhile in control group, we observed abscesses areas with typical necrosis including trophozoites and neutrophils. Macrophages were differentially distributed surrounding abscess area in control and vehicle groups, but equally located in and outside of the abscesses in sympathectomized rat. No significant differences were observed on NK cells in analysed groups. In cytokines quantification studies, we observed down-expression of IFN-g and TNF-a, moreover, we found overexpression of IL-10 in sympathectomized and ALA group. In conclusion, our results suggest that elimination of sympathetic nerve fibers in a model rat of amebic liver abscess induces reduction of the innate immune response and presence of amebas through the liver at seven days post-inoculation.

AB - © 2015, Universidad de la Frontera. All rights reserved. All organs of the immune system are innervated and almost all neurotransmitter receptors are present on immune cells. We studied the effects of sympathetic innervation in the development of amebic liver abscess (ALA) in rats. Our results showed that lack of sympathetic innervation promote a decrease in size of ALA. We found scarce amoebas, increased the number of neutrophils and a few collagen fibers surrounding the abscess, meanwhile in control group, we observed abscesses areas with typical necrosis including trophozoites and neutrophils. Macrophages were differentially distributed surrounding abscess area in control and vehicle groups, but equally located in and outside of the abscesses in sympathectomized rat. No significant differences were observed on NK cells in analysed groups. In cytokines quantification studies, we observed down-expression of IFN-g and TNF-a, moreover, we found overexpression of IL-10 in sympathectomized and ALA group. In conclusion, our results suggest that elimination of sympathetic nerve fibers in a model rat of amebic liver abscess induces reduction of the innate immune response and presence of amebas through the liver at seven days post-inoculation.

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DO - 10.4067/S0717-95022015000100034

M3 - Article

SP - 213

EP - 221

JO - International Journal of Morphology

JF - International Journal of Morphology

SN - 0717-9367

ER -