TY - JOUR
T1 - Amebic liver abscess in rat
T2 - Morphological evidence of innate immune modulation by the sympathetic nervous system
AU - Muñoz-Ortega, Martín Humberto
AU - Cervantes-García, Daniel
AU - Quintanar-Stephano, Andrés
AU - Campos-Esparza, María Del Rosario
AU - García-Lorenzana, Mario
AU - Campos-Rodríguez, R.
AU - Rodríguez, M. G.
AU - Ventura-Juárez, Javier
N1 - Publisher Copyright:
© 2015, Universidad de la Frontera. All rights reserved.
PY - 2015
Y1 - 2015
N2 - All organs of the immune system are innervated and almost all neurotransmitter receptors are present on immune cells. We studied the effects of sympathetic innervation in the development of amebic liver abscess (ALA) in rats. Our results showed that lack of sympathetic innervation promote a decrease in size of ALA. We found scarce amoebas, increased the number of neutrophils and a few collagen fibers surrounding the abscess, meanwhile in control group, we observed abscesses areas with typical necrosis including trophozoites and neutrophils. Macrophages were differentially distributed surrounding abscess area in control and vehicle groups, but equally located in and outside of the abscesses in sympathectomized rat. No significant differences were observed on NK cells in analysed groups. In cytokines quantification studies, we observed down-expression of IFN-g and TNF-a, moreover, we found overexpression of IL-10 in sympathectomized and ALA group. In conclusion, our results suggest that elimination of sympathetic nerve fibers in a model rat of amebic liver abscess induces reduction of the innate immune response and presence of amebas through the liver at seven days post-inoculation.
AB - All organs of the immune system are innervated and almost all neurotransmitter receptors are present on immune cells. We studied the effects of sympathetic innervation in the development of amebic liver abscess (ALA) in rats. Our results showed that lack of sympathetic innervation promote a decrease in size of ALA. We found scarce amoebas, increased the number of neutrophils and a few collagen fibers surrounding the abscess, meanwhile in control group, we observed abscesses areas with typical necrosis including trophozoites and neutrophils. Macrophages were differentially distributed surrounding abscess area in control and vehicle groups, but equally located in and outside of the abscesses in sympathectomized rat. No significant differences were observed on NK cells in analysed groups. In cytokines quantification studies, we observed down-expression of IFN-g and TNF-a, moreover, we found overexpression of IL-10 in sympathectomized and ALA group. In conclusion, our results suggest that elimination of sympathetic nerve fibers in a model rat of amebic liver abscess induces reduction of the innate immune response and presence of amebas through the liver at seven days post-inoculation.
KW - Amebic liver abscess
KW - E. histolytica
KW - Rat
KW - Sympathectomy
UR - http://www.scopus.com/inward/record.url?scp=84927599966&partnerID=8YFLogxK
U2 - 10.4067/S0717-95022015000100034
DO - 10.4067/S0717-95022015000100034
M3 - Artículo
SN - 0717-9367
VL - 33
SP - 213
EP - 221
JO - International Journal of Morphology
JF - International Journal of Morphology
IS - 1
ER -