Alterations to DNA, apoptosis and oxidative damage induced by sucralose in blood cells of Cyprinus carpio

Gerardo Heredia-García, Leobardo Manuel Gómez-Oliván, José Manuel Orozco-Hernández, Marlenee Luja-Mondragón, Hariz Islas-Flores, Nely SanJuan-Reyes, Marcela Galar-Martínez, Sandra García-Medina, Octavio Dublán-García

Research output: Contribution to journalArticle

Abstract

Sucralose (SUC) is an organochlorine that is used as a common sweetener in different dietary products around the world. Its extended use and production have led to this product is released into the environment in concentrations ranging from ng L−1 to μg L−1 in surface waters, groundwaters, wastewater treatment plants and ocean waters. A previous study carried out by our research team demonstrated that SUC is capable of inducing oxidative stress in Cyprinus carpio at environmentally-relevant concentrations. The aim of this study was to evaluate if SUC was capable of inducing alterations to DNA, apoptosis, and oxidative damage in the blood cells of C. carpio. Carps were exposed to two environmentally-relevant concentrations (0.05 and 155 μg L−1) of SUC, and the following biomarkers were determined: comet assay, micronucleus test (MN), caspase-3 activity, TUNEL assay, hydroperoxide content, lipid peroxidation level, protein carbonyl content and superoxide dismutase and catalase activities. Results obtained showed that SUC is capable of inducing DNA damage. A maximum increase of 35% and 23% were observed for c1 and c2, respectively in the comet assay; increases of 586% and 507.7% for c1 and c2, respectively, were found at 72 h through the MN test. The activity of caspase-3 showed a greater response for c1 and c2 at 96 h, with 271% and 493.5%, respectively. TUNEL assay also showed the highest response at 96 h, with 51.8 for c1 and 72.9 for c2; c1 y c2 were able to induce oxidative stress with the highest expression at 72 h. A correlation between DNA damage biomarkers, apoptosis and plasma levels of SUC in both concentrations were observed. With the data obtained, we can conclude that SUC, at environmentally-relevant concentrations, was capable of generating DNA alterations, apoptosis and oxidative stress in blood cells in common carp.

Original languageEnglish
Pages (from-to)411-421
Number of pages11
JournalScience of the Total Environment
Volume692
DOIs
StatePublished - 20 Nov 2019

Fingerprint

trichlorosucrose
apoptosis
Cell death
Oxidative stress
Assays
DNA
Blood
blood
Cells
assay
Apoptosis
damage
Biomarkers
comet
biomarker
Caspase 3
Surface waters
organochlorine
Wastewater treatment
Lipids

Keywords

  • Apoptosis
  • Common carp
  • DNA alterations
  • Oxidative stress
  • Sweeteners

Cite this

Heredia-García, G., Gómez-Oliván, L. M., Orozco-Hernández, J. M., Luja-Mondragón, M., Islas-Flores, H., SanJuan-Reyes, N., ... Dublán-García, O. (2019). Alterations to DNA, apoptosis and oxidative damage induced by sucralose in blood cells of Cyprinus carpio. Science of the Total Environment, 692, 411-421. https://doi.org/10.1016/j.scitotenv.2019.07.165
Heredia-García, Gerardo ; Gómez-Oliván, Leobardo Manuel ; Orozco-Hernández, José Manuel ; Luja-Mondragón, Marlenee ; Islas-Flores, Hariz ; SanJuan-Reyes, Nely ; Galar-Martínez, Marcela ; García-Medina, Sandra ; Dublán-García, Octavio. / Alterations to DNA, apoptosis and oxidative damage induced by sucralose in blood cells of Cyprinus carpio. In: Science of the Total Environment. 2019 ; Vol. 692. pp. 411-421.
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abstract = "Sucralose (SUC) is an organochlorine that is used as a common sweetener in different dietary products around the world. Its extended use and production have led to this product is released into the environment in concentrations ranging from ng L−1 to μg L−1 in surface waters, groundwaters, wastewater treatment plants and ocean waters. A previous study carried out by our research team demonstrated that SUC is capable of inducing oxidative stress in Cyprinus carpio at environmentally-relevant concentrations. The aim of this study was to evaluate if SUC was capable of inducing alterations to DNA, apoptosis, and oxidative damage in the blood cells of C. carpio. Carps were exposed to two environmentally-relevant concentrations (0.05 and 155 μg L−1) of SUC, and the following biomarkers were determined: comet assay, micronucleus test (MN), caspase-3 activity, TUNEL assay, hydroperoxide content, lipid peroxidation level, protein carbonyl content and superoxide dismutase and catalase activities. Results obtained showed that SUC is capable of inducing DNA damage. A maximum increase of 35{\%} and 23{\%} were observed for c1 and c2, respectively in the comet assay; increases of 586{\%} and 507.7{\%} for c1 and c2, respectively, were found at 72 h through the MN test. The activity of caspase-3 showed a greater response for c1 and c2 at 96 h, with 271{\%} and 493.5{\%}, respectively. TUNEL assay also showed the highest response at 96 h, with 51.8 for c1 and 72.9 for c2; c1 y c2 were able to induce oxidative stress with the highest expression at 72 h. A correlation between DNA damage biomarkers, apoptosis and plasma levels of SUC in both concentrations were observed. With the data obtained, we can conclude that SUC, at environmentally-relevant concentrations, was capable of generating DNA alterations, apoptosis and oxidative stress in blood cells in common carp.",
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Heredia-García, G, Gómez-Oliván, LM, Orozco-Hernández, JM, Luja-Mondragón, M, Islas-Flores, H, SanJuan-Reyes, N, Galar-Martínez, M, García-Medina, S & Dublán-García, O 2019, 'Alterations to DNA, apoptosis and oxidative damage induced by sucralose in blood cells of Cyprinus carpio', Science of the Total Environment, vol. 692, pp. 411-421. https://doi.org/10.1016/j.scitotenv.2019.07.165

Alterations to DNA, apoptosis and oxidative damage induced by sucralose in blood cells of Cyprinus carpio. / Heredia-García, Gerardo; Gómez-Oliván, Leobardo Manuel; Orozco-Hernández, José Manuel; Luja-Mondragón, Marlenee; Islas-Flores, Hariz; SanJuan-Reyes, Nely; Galar-Martínez, Marcela; García-Medina, Sandra; Dublán-García, Octavio.

In: Science of the Total Environment, Vol. 692, 20.11.2019, p. 411-421.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Alterations to DNA, apoptosis and oxidative damage induced by sucralose in blood cells of Cyprinus carpio

AU - Heredia-García, Gerardo

AU - Gómez-Oliván, Leobardo Manuel

AU - Orozco-Hernández, José Manuel

AU - Luja-Mondragón, Marlenee

AU - Islas-Flores, Hariz

AU - SanJuan-Reyes, Nely

AU - Galar-Martínez, Marcela

AU - García-Medina, Sandra

AU - Dublán-García, Octavio

PY - 2019/11/20

Y1 - 2019/11/20

N2 - Sucralose (SUC) is an organochlorine that is used as a common sweetener in different dietary products around the world. Its extended use and production have led to this product is released into the environment in concentrations ranging from ng L−1 to μg L−1 in surface waters, groundwaters, wastewater treatment plants and ocean waters. A previous study carried out by our research team demonstrated that SUC is capable of inducing oxidative stress in Cyprinus carpio at environmentally-relevant concentrations. The aim of this study was to evaluate if SUC was capable of inducing alterations to DNA, apoptosis, and oxidative damage in the blood cells of C. carpio. Carps were exposed to two environmentally-relevant concentrations (0.05 and 155 μg L−1) of SUC, and the following biomarkers were determined: comet assay, micronucleus test (MN), caspase-3 activity, TUNEL assay, hydroperoxide content, lipid peroxidation level, protein carbonyl content and superoxide dismutase and catalase activities. Results obtained showed that SUC is capable of inducing DNA damage. A maximum increase of 35% and 23% were observed for c1 and c2, respectively in the comet assay; increases of 586% and 507.7% for c1 and c2, respectively, were found at 72 h through the MN test. The activity of caspase-3 showed a greater response for c1 and c2 at 96 h, with 271% and 493.5%, respectively. TUNEL assay also showed the highest response at 96 h, with 51.8 for c1 and 72.9 for c2; c1 y c2 were able to induce oxidative stress with the highest expression at 72 h. A correlation between DNA damage biomarkers, apoptosis and plasma levels of SUC in both concentrations were observed. With the data obtained, we can conclude that SUC, at environmentally-relevant concentrations, was capable of generating DNA alterations, apoptosis and oxidative stress in blood cells in common carp.

AB - Sucralose (SUC) is an organochlorine that is used as a common sweetener in different dietary products around the world. Its extended use and production have led to this product is released into the environment in concentrations ranging from ng L−1 to μg L−1 in surface waters, groundwaters, wastewater treatment plants and ocean waters. A previous study carried out by our research team demonstrated that SUC is capable of inducing oxidative stress in Cyprinus carpio at environmentally-relevant concentrations. The aim of this study was to evaluate if SUC was capable of inducing alterations to DNA, apoptosis, and oxidative damage in the blood cells of C. carpio. Carps were exposed to two environmentally-relevant concentrations (0.05 and 155 μg L−1) of SUC, and the following biomarkers were determined: comet assay, micronucleus test (MN), caspase-3 activity, TUNEL assay, hydroperoxide content, lipid peroxidation level, protein carbonyl content and superoxide dismutase and catalase activities. Results obtained showed that SUC is capable of inducing DNA damage. A maximum increase of 35% and 23% were observed for c1 and c2, respectively in the comet assay; increases of 586% and 507.7% for c1 and c2, respectively, were found at 72 h through the MN test. The activity of caspase-3 showed a greater response for c1 and c2 at 96 h, with 271% and 493.5%, respectively. TUNEL assay also showed the highest response at 96 h, with 51.8 for c1 and 72.9 for c2; c1 y c2 were able to induce oxidative stress with the highest expression at 72 h. A correlation between DNA damage biomarkers, apoptosis and plasma levels of SUC in both concentrations were observed. With the data obtained, we can conclude that SUC, at environmentally-relevant concentrations, was capable of generating DNA alterations, apoptosis and oxidative stress in blood cells in common carp.

KW - Apoptosis

KW - Common carp

KW - DNA alterations

KW - Oxidative stress

KW - Sweeteners

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Heredia-García G, Gómez-Oliván LM, Orozco-Hernández JM, Luja-Mondragón M, Islas-Flores H, SanJuan-Reyes N et al. Alterations to DNA, apoptosis and oxidative damage induced by sucralose in blood cells of Cyprinus carpio. Science of the Total Environment. 2019 Nov 20;692:411-421. https://doi.org/10.1016/j.scitotenv.2019.07.165