TY - JOUR
T1 - Adventitious root cultures of Castilleja tenuiflora Benth. as a source of phenylethanoid glycosides
AU - Gómez-Aguirre, Yenny Adriana
AU - Zamilpa, Alejandro
AU - González-Cortazar, Manasés
AU - Trejo-Tapia, Gabriela
N1 - Funding Information:
This work was supported by the Secretaría de Investigación y Posgrado del Instituto Politécnico Nacional (IPN) (Grants 20090311 , 20100308 , and 20110153 ) and by Consejo Nacional de Ciencia y Tecnología (CONACyT-México) (Grant 100202 ). Y.A. Gómez-Aguirre is indebted to CONACyT for the doctoral fellowship awarded and Programa Institucional de Formación de Investigadores (PIFI-IPN). G. Trejo-Tapia is grateful to SIBE-IPN and EDI-IPN.
PY - 2012/3
Y1 - 2012/3
N2 - Castilleja tenuiflora is a highly valued medicinal plant that grows in pine-oak woods in Mexico. In this study, we identified for the first time verbascoside and isoverbascoside as the major phenylethanoid glycosides (PhGs) in C. tenuiflora. These compounds have proven biological activities, including anti-inflammatory, antioxidant, and cytotoxic activities, which may be related to the traditional uses of this plant. We developed a reverse-phase high-performance liquid chromatography (RP-HPLC) procedure to analyze PhGs, and determined their concentrations in various different tissues of wild plants. Verbascoside accumulated mainly in roots and inflorescences (9.23 and 7.88mgg -1 dry biomass, respectively), while isoverbascoside accumulated mainly in the roots (7.13mgg -1 dry biomass). To provide an alternative source of material for production of bioactive compounds, we established in vitro adventitious root cultures in which roots were grown in B5 medium containing either 10μM indole 3-acetic acid (IAA) or 10μM α-naphthaleneacetic acid (NAA). The greatest dry biomass yield (30gL -1) was achieved at 30 days after transfer of roots into IAA-containing medium. The highest specific yields of PhGs were also obtained using this auxin; the maximum level of verbascoside was 14.62mgg -1 dry root biomass (438.6mgL -1) at 30 days after root transfer, and the maximum yield of isoverbascoside was 37.32mgg -1 dry root biomass (522.48mgL -1) at 23 days after root transfer. Adventitious root cultures of C. tenuiflora are a promising system for further studies on scale-up and phenylethanoid glycosides biosynthesis.
AB - Castilleja tenuiflora is a highly valued medicinal plant that grows in pine-oak woods in Mexico. In this study, we identified for the first time verbascoside and isoverbascoside as the major phenylethanoid glycosides (PhGs) in C. tenuiflora. These compounds have proven biological activities, including anti-inflammatory, antioxidant, and cytotoxic activities, which may be related to the traditional uses of this plant. We developed a reverse-phase high-performance liquid chromatography (RP-HPLC) procedure to analyze PhGs, and determined their concentrations in various different tissues of wild plants. Verbascoside accumulated mainly in roots and inflorescences (9.23 and 7.88mgg -1 dry biomass, respectively), while isoverbascoside accumulated mainly in the roots (7.13mgg -1 dry biomass). To provide an alternative source of material for production of bioactive compounds, we established in vitro adventitious root cultures in which roots were grown in B5 medium containing either 10μM indole 3-acetic acid (IAA) or 10μM α-naphthaleneacetic acid (NAA). The greatest dry biomass yield (30gL -1) was achieved at 30 days after transfer of roots into IAA-containing medium. The highest specific yields of PhGs were also obtained using this auxin; the maximum level of verbascoside was 14.62mgg -1 dry root biomass (438.6mgL -1) at 30 days after root transfer, and the maximum yield of isoverbascoside was 37.32mgg -1 dry root biomass (522.48mgL -1) at 23 days after root transfer. Adventitious root cultures of C. tenuiflora are a promising system for further studies on scale-up and phenylethanoid glycosides biosynthesis.
KW - Acteoside
KW - Adventitious root cultures
KW - Auxins
KW - Castilleja tenuiflora
KW - Isoacteoside
KW - Phenylethanoid glycosides
UR - http://www.scopus.com/inward/record.url?scp=80054095474&partnerID=8YFLogxK
U2 - 10.1016/j.indcrop.2011.09.005
DO - 10.1016/j.indcrop.2011.09.005
M3 - Artículo
SN - 0926-6690
VL - 36
SP - 188
EP - 195
JO - Industrial Crops and Products
JF - Industrial Crops and Products
IS - 1
ER -