Adhesion forces of biofilms developed in vitro from clinical strains of skin wounds

Elizabeth Alvarado-Gomez, Mario Perez-Diaz, Donato Valdez-Perez, Jaime Ruiz-Garcia, Martin Magaña-Aquino, Gabriel Martinez-Castañon, Fidel Martinez-Gutierrez

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

© 2017 Elsevier B.V. A biofilm is a very complex consortium formed by a mix of different microorganisms, which have become an important health problem, because its formation is a resistance mechanism used by bacteria against antibiotics or the immune system. In this work, we show differences between some physicochemical properties of biofilms in mono- and multi-species, formed by bacteria from clinical samples of infected chronic wounds. Of the most prevalent bacteria in wounds, two mono- and one multi-species biofilms were developed in vitro by Drip Flow Reactor: one biofilm was developed by S. aureus, other by P. aeruginosa, and a third one by the mix of both strains. With these biofilms, we determined microbial growth by plate counting, and their physicochemical characterization by Atomic Force Microscopy, Raman Micro-Spectroscopy and Scanning Electron Microscopy. We found that the viability of S. aureus was less than P. aeruginosa in multi-species biofilm. However, the adhesion force of S. aureus is much higher than that of P. aeruginosa, but it decreased while that of P. aeruginosa increased in the multi-species biofilm. In addition, we found free pyrimidines functional groups in the P. aeruginosa biofilm and its mix with S. aureus. Surprisingly, each bacterium alone formed single layer biofilms, while the mix bacteria formed a multilayer biofilm at the same observation time. Our results show the necessity to evaluate biofilms from clinically isolated strains and have a better understanding of the adhesion forces of bacteria in biofilm multispecies, which could be of prime importance in developing more effective treatments against biofilm formation.
Original languageAmerican English
Pages (from-to)336-344
Number of pages301
JournalMaterials Science and Engineering C
DOIs
StatePublished - 1 Jan 2018

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biofilms
Biofilms
adhesion
biofilm
skin
Skin
Adhesion
bacteria
Bacteria
bacterium
immune systems
Pyrimidines
atomic force microscopy
antibiotics
Immune system
pyrimidines
immune system
Antibiotics
microorganisms
physicochemical property

Cite this

Alvarado-Gomez, E., Perez-Diaz, M., Valdez-Perez, D., Ruiz-Garcia, J., Magaña-Aquino, M., Martinez-Castañon, G., & Martinez-Gutierrez, F. (2018). Adhesion forces of biofilms developed in vitro from clinical strains of skin wounds. Materials Science and Engineering C, 336-344. https://doi.org/10.1016/j.msec.2017.08.028
Alvarado-Gomez, Elizabeth ; Perez-Diaz, Mario ; Valdez-Perez, Donato ; Ruiz-Garcia, Jaime ; Magaña-Aquino, Martin ; Martinez-Castañon, Gabriel ; Martinez-Gutierrez, Fidel. / Adhesion forces of biofilms developed in vitro from clinical strains of skin wounds. In: Materials Science and Engineering C. 2018 ; pp. 336-344.
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Alvarado-Gomez, E, Perez-Diaz, M, Valdez-Perez, D, Ruiz-Garcia, J, Magaña-Aquino, M, Martinez-Castañon, G & Martinez-Gutierrez, F 2018, 'Adhesion forces of biofilms developed in vitro from clinical strains of skin wounds', Materials Science and Engineering C, pp. 336-344. https://doi.org/10.1016/j.msec.2017.08.028

Adhesion forces of biofilms developed in vitro from clinical strains of skin wounds. / Alvarado-Gomez, Elizabeth; Perez-Diaz, Mario; Valdez-Perez, Donato; Ruiz-Garcia, Jaime; Magaña-Aquino, Martin; Martinez-Castañon, Gabriel; Martinez-Gutierrez, Fidel.

In: Materials Science and Engineering C, 01.01.2018, p. 336-344.

Research output: Contribution to journalArticle

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AU - Alvarado-Gomez, Elizabeth

AU - Perez-Diaz, Mario

AU - Valdez-Perez, Donato

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AU - Magaña-Aquino, Martin

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