TY - JOUR
T1 - Adherence of Ornithobacterium rhinotracheale to chicken embryo lung cells as a pathogenic mechanism
AU - De la Rosa-Ramos, Miguel A.
AU - Muñoz-Solís, Karina
AU - Palma-Zepeda, Mario
AU - Gutierrez-Castillo, Adriana C.
AU - López Villegas, Edgar O.
AU - Guerra-Infante, Fernando M.
AU - Castro-Escarpulli, Graciela
N1 - Publisher Copyright:
© 2017 Houghton Trust Ltd.
PY - 2018/3/4
Y1 - 2018/3/4
N2 - Ornithobacterium rhinotracheale is a bacterium that causes respiratory disease in birds and it has been isolated in countries with a large poultry production, including Mexico. The pathogenicity mechanisms of this bacterium have not been completely elucidated yet. The capacity of the bacterium to adhere to epithelial cells of chicken in vitro has been evidenced, and since this bacterium has been isolated from the lungs and air sacs of several avian species, the aim of this study was to determine if this bacterium can adhere to chicken lung cells. We used five O. rhinotracheale reference serovars (A–E) that were in contact with primary lung cells cultured from a 19-day-old chicken embryo. O. rhinotracheale adherence was evaluated through optical and transmission electron microscopies. The results revealed that O. rhinotracheale is capable of adhering to chicken embryo lung cells within 3 h of incubation with a diffuse adherence pattern. The adherence percentages of the chicken embryo lung cells were 51–96% according to the serovar of the bacterium. Relative adherence was from 4 to 8 bacteria per cell. Transmission electron microscope data revealed intracellular bacteria inside a vacuole in less than 3 h of incubation.
AB - Ornithobacterium rhinotracheale is a bacterium that causes respiratory disease in birds and it has been isolated in countries with a large poultry production, including Mexico. The pathogenicity mechanisms of this bacterium have not been completely elucidated yet. The capacity of the bacterium to adhere to epithelial cells of chicken in vitro has been evidenced, and since this bacterium has been isolated from the lungs and air sacs of several avian species, the aim of this study was to determine if this bacterium can adhere to chicken lung cells. We used five O. rhinotracheale reference serovars (A–E) that were in contact with primary lung cells cultured from a 19-day-old chicken embryo. O. rhinotracheale adherence was evaluated through optical and transmission electron microscopies. The results revealed that O. rhinotracheale is capable of adhering to chicken embryo lung cells within 3 h of incubation with a diffuse adherence pattern. The adherence percentages of the chicken embryo lung cells were 51–96% according to the serovar of the bacterium. Relative adherence was from 4 to 8 bacteria per cell. Transmission electron microscope data revealed intracellular bacteria inside a vacuole in less than 3 h of incubation.
KW - Mexico
KW - Ornithobacterium rhinotracheale
KW - adherence
KW - avian diseases
KW - bacterial infections
KW - birds
KW - pathogenicity mechanisms
KW - poultry industry
KW - respiratory disease
UR - http://www.scopus.com/inward/record.url?scp=85032675584&partnerID=8YFLogxK
U2 - 10.1080/03079457.2017.1390208
DO - 10.1080/03079457.2017.1390208
M3 - Artículo
C2 - 29016186
SN - 0307-9457
VL - 47
SP - 172
EP - 178
JO - Avian Pathology
JF - Avian Pathology
IS - 2
ER -