Activity of Thioallyl Compounds From Garlic Against Giardia duodenalis Trophozoites and in Experimental Giardiasis

Raúl Argüello-García, Mariana de la Vega-Arnaud, Iraís J. Loredo-Rodríguez, Adriana M. Mejía-Corona, Elizabeth Melgarejo-Trejo, Eulogia A. Espinoza-Contreras, Rocío Fonseca-Liñán, Arturo González-Robles, Nury Pérez-Hernández, M. Guadalupe Ortega-Pierres

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

Fresh aqueous extracts (AGEs) and several thioallyl compounds (TACs) from garlic have an important antimicrobial activity that likely involves their interaction with exposed thiol groups at single aminoacids or target proteins. Since these groups are present in Giardia duodenalis trophozoites, in this work we evaluated the anti-giardial activity of AGE and several garlic's TACs. In vitro susceptibility assays showed that AGE affected trophozoite viability initially by a mechanism impairing cell integrity and oxidoreductase activities while diesterase activities were abrogated at higher AGE concentrations. The giardicidal activities of seven TACs were related to the molecular descriptor HOMO (Highest Occupied Molecular Orbital) energy and with their capacity to modify the -SH groups exposed in giardial proteins. Interestingly, the activity of several cysteine proteases in trophozoite lysates was inhibited by representative TACs as well as the cytopathic effect of the virulence factor giardipain-1. Of these, allicin showed the highest anti-giardial activity, the lower HOMO value, the highest thiol-modifying activity and the greatest inhibition of cysteine proteases. Allicin had a cytolytic mechanism in trophozoites with subsequent impairment of diesterase and oxidoreductase activities in a similar way to AGE. In addition, by electron microscopy a marked destruction of plasma membrane and endomembranes was observed in allicin-treated trophozoites while cytoskeletal elements were not affected. In further flow cytometry analyses pro-apoptotic effects of allicin concomitant to partial cell cycle arrest at G2 phase with the absence of oxidative stress were observed. In experimental infections of gerbils, the intragastric administration of AGE or allicin decreased parasite numbers and eliminated trophozoites in experimentally infected animals, respectively. These data suggest a potential use of TACs from garlic against G. duodenalis and in the treatment of giardiasis along with their additional benefits in the host's health.
Original languageAmerican English
Pages (from-to)353
Number of pages1
JournalFrontiers in Cellular and Infection Microbiology
DOIs
StatePublished - 1 Jan 2018

Fingerprint

Giardiasis
Giardia lamblia
Trophozoites
Garlic
Cysteine Proteases
Sulfhydryl Compounds
Oxidoreductases
Gerbillinae
G2 Phase
Virulence Factors
Cell Cycle Checkpoints
Electron Microscopy
Flow Cytometry
Parasites
Proteins
Oxidative Stress
Cell Membrane
allicin
Health
Infection

Cite this

Argüello-García, R., de la Vega-Arnaud, M., Loredo-Rodríguez, I. J., Mejía-Corona, A. M., Melgarejo-Trejo, E., Espinoza-Contreras, E. A., ... Ortega-Pierres, M. G. (2018). Activity of Thioallyl Compounds From Garlic Against Giardia duodenalis Trophozoites and in Experimental Giardiasis. Frontiers in Cellular and Infection Microbiology, 353. https://doi.org/10.3389/fcimb.2018.00353
Argüello-García, Raúl ; de la Vega-Arnaud, Mariana ; Loredo-Rodríguez, Iraís J. ; Mejía-Corona, Adriana M. ; Melgarejo-Trejo, Elizabeth ; Espinoza-Contreras, Eulogia A. ; Fonseca-Liñán, Rocío ; González-Robles, Arturo ; Pérez-Hernández, Nury ; Ortega-Pierres, M. Guadalupe. / Activity of Thioallyl Compounds From Garlic Against Giardia duodenalis Trophozoites and in Experimental Giardiasis. In: Frontiers in Cellular and Infection Microbiology. 2018 ; pp. 353.
@article{920af89ff731491da05963f192c54540,
title = "Activity of Thioallyl Compounds From Garlic Against Giardia duodenalis Trophozoites and in Experimental Giardiasis",
abstract = "Fresh aqueous extracts (AGEs) and several thioallyl compounds (TACs) from garlic have an important antimicrobial activity that likely involves their interaction with exposed thiol groups at single aminoacids or target proteins. Since these groups are present in Giardia duodenalis trophozoites, in this work we evaluated the anti-giardial activity of AGE and several garlic's TACs. In vitro susceptibility assays showed that AGE affected trophozoite viability initially by a mechanism impairing cell integrity and oxidoreductase activities while diesterase activities were abrogated at higher AGE concentrations. The giardicidal activities of seven TACs were related to the molecular descriptor HOMO (Highest Occupied Molecular Orbital) energy and with their capacity to modify the -SH groups exposed in giardial proteins. Interestingly, the activity of several cysteine proteases in trophozoite lysates was inhibited by representative TACs as well as the cytopathic effect of the virulence factor giardipain-1. Of these, allicin showed the highest anti-giardial activity, the lower HOMO value, the highest thiol-modifying activity and the greatest inhibition of cysteine proteases. Allicin had a cytolytic mechanism in trophozoites with subsequent impairment of diesterase and oxidoreductase activities in a similar way to AGE. In addition, by electron microscopy a marked destruction of plasma membrane and endomembranes was observed in allicin-treated trophozoites while cytoskeletal elements were not affected. In further flow cytometry analyses pro-apoptotic effects of allicin concomitant to partial cell cycle arrest at G2 phase with the absence of oxidative stress were observed. In experimental infections of gerbils, the intragastric administration of AGE or allicin decreased parasite numbers and eliminated trophozoites in experimentally infected animals, respectively. These data suggest a potential use of TACs from garlic against G. duodenalis and in the treatment of giardiasis along with their additional benefits in the host's health.",
author = "Ra{\'u}l Arg{\"u}ello-Garc{\'i}a and {de la Vega-Arnaud}, Mariana and Loredo-Rodr{\'i}guez, {Ira{\'i}s J.} and Mej{\'i}a-Corona, {Adriana M.} and Elizabeth Melgarejo-Trejo and Espinoza-Contreras, {Eulogia A.} and Roc{\'i}o Fonseca-Li{\~n}{\'a}n and Arturo Gonz{\'a}lez-Robles and Nury P{\'e}rez-Hern{\'a}ndez and Ortega-Pierres, {M. Guadalupe}",
year = "2018",
month = "1",
day = "1",
doi = "10.3389/fcimb.2018.00353",
language = "American English",
pages = "353",
journal = "Frontiers in Cellular and Infection Microbiology",
issn = "2235-2988",
publisher = "Frontiers Media S.A.",

}

Argüello-García, R, de la Vega-Arnaud, M, Loredo-Rodríguez, IJ, Mejía-Corona, AM, Melgarejo-Trejo, E, Espinoza-Contreras, EA, Fonseca-Liñán, R, González-Robles, A, Pérez-Hernández, N & Ortega-Pierres, MG 2018, 'Activity of Thioallyl Compounds From Garlic Against Giardia duodenalis Trophozoites and in Experimental Giardiasis', Frontiers in Cellular and Infection Microbiology, pp. 353. https://doi.org/10.3389/fcimb.2018.00353

Activity of Thioallyl Compounds From Garlic Against Giardia duodenalis Trophozoites and in Experimental Giardiasis. / Argüello-García, Raúl; de la Vega-Arnaud, Mariana; Loredo-Rodríguez, Iraís J.; Mejía-Corona, Adriana M.; Melgarejo-Trejo, Elizabeth; Espinoza-Contreras, Eulogia A.; Fonseca-Liñán, Rocío; González-Robles, Arturo; Pérez-Hernández, Nury; Ortega-Pierres, M. Guadalupe.

In: Frontiers in Cellular and Infection Microbiology, 01.01.2018, p. 353.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Activity of Thioallyl Compounds From Garlic Against Giardia duodenalis Trophozoites and in Experimental Giardiasis

AU - Argüello-García, Raúl

AU - de la Vega-Arnaud, Mariana

AU - Loredo-Rodríguez, Iraís J.

AU - Mejía-Corona, Adriana M.

AU - Melgarejo-Trejo, Elizabeth

AU - Espinoza-Contreras, Eulogia A.

AU - Fonseca-Liñán, Rocío

AU - González-Robles, Arturo

AU - Pérez-Hernández, Nury

AU - Ortega-Pierres, M. Guadalupe

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Fresh aqueous extracts (AGEs) and several thioallyl compounds (TACs) from garlic have an important antimicrobial activity that likely involves their interaction with exposed thiol groups at single aminoacids or target proteins. Since these groups are present in Giardia duodenalis trophozoites, in this work we evaluated the anti-giardial activity of AGE and several garlic's TACs. In vitro susceptibility assays showed that AGE affected trophozoite viability initially by a mechanism impairing cell integrity and oxidoreductase activities while diesterase activities were abrogated at higher AGE concentrations. The giardicidal activities of seven TACs were related to the molecular descriptor HOMO (Highest Occupied Molecular Orbital) energy and with their capacity to modify the -SH groups exposed in giardial proteins. Interestingly, the activity of several cysteine proteases in trophozoite lysates was inhibited by representative TACs as well as the cytopathic effect of the virulence factor giardipain-1. Of these, allicin showed the highest anti-giardial activity, the lower HOMO value, the highest thiol-modifying activity and the greatest inhibition of cysteine proteases. Allicin had a cytolytic mechanism in trophozoites with subsequent impairment of diesterase and oxidoreductase activities in a similar way to AGE. In addition, by electron microscopy a marked destruction of plasma membrane and endomembranes was observed in allicin-treated trophozoites while cytoskeletal elements were not affected. In further flow cytometry analyses pro-apoptotic effects of allicin concomitant to partial cell cycle arrest at G2 phase with the absence of oxidative stress were observed. In experimental infections of gerbils, the intragastric administration of AGE or allicin decreased parasite numbers and eliminated trophozoites in experimentally infected animals, respectively. These data suggest a potential use of TACs from garlic against G. duodenalis and in the treatment of giardiasis along with their additional benefits in the host's health.

AB - Fresh aqueous extracts (AGEs) and several thioallyl compounds (TACs) from garlic have an important antimicrobial activity that likely involves their interaction with exposed thiol groups at single aminoacids or target proteins. Since these groups are present in Giardia duodenalis trophozoites, in this work we evaluated the anti-giardial activity of AGE and several garlic's TACs. In vitro susceptibility assays showed that AGE affected trophozoite viability initially by a mechanism impairing cell integrity and oxidoreductase activities while diesterase activities were abrogated at higher AGE concentrations. The giardicidal activities of seven TACs were related to the molecular descriptor HOMO (Highest Occupied Molecular Orbital) energy and with their capacity to modify the -SH groups exposed in giardial proteins. Interestingly, the activity of several cysteine proteases in trophozoite lysates was inhibited by representative TACs as well as the cytopathic effect of the virulence factor giardipain-1. Of these, allicin showed the highest anti-giardial activity, the lower HOMO value, the highest thiol-modifying activity and the greatest inhibition of cysteine proteases. Allicin had a cytolytic mechanism in trophozoites with subsequent impairment of diesterase and oxidoreductase activities in a similar way to AGE. In addition, by electron microscopy a marked destruction of plasma membrane and endomembranes was observed in allicin-treated trophozoites while cytoskeletal elements were not affected. In further flow cytometry analyses pro-apoptotic effects of allicin concomitant to partial cell cycle arrest at G2 phase with the absence of oxidative stress were observed. In experimental infections of gerbils, the intragastric administration of AGE or allicin decreased parasite numbers and eliminated trophozoites in experimentally infected animals, respectively. These data suggest a potential use of TACs from garlic against G. duodenalis and in the treatment of giardiasis along with their additional benefits in the host's health.

U2 - 10.3389/fcimb.2018.00353

DO - 10.3389/fcimb.2018.00353

M3 - Article

SP - 353

JO - Frontiers in Cellular and Infection Microbiology

JF - Frontiers in Cellular and Infection Microbiology

SN - 2235-2988

ER -

Argüello-García R, de la Vega-Arnaud M, Loredo-Rodríguez IJ, Mejía-Corona AM, Melgarejo-Trejo E, Espinoza-Contreras EA et al. Activity of Thioallyl Compounds From Garlic Against Giardia duodenalis Trophozoites and in Experimental Giardiasis. Frontiers in Cellular and Infection Microbiology. 2018 Jan 1;353. https://doi.org/10.3389/fcimb.2018.00353