TY - JOUR
T1 - Activities of α-asarone in various animal seizure models and in biochemical assays might be essentially accounted for by antioxidant properties
AU - Pages, Nicole
AU - Maurois, Pierre
AU - Delplanque, Bernadette
AU - Bac, Pierre
AU - Stables, James P.
AU - Tamariz, Joaqún
AU - Chamorro, Germán
AU - Vamecq, Joseph
N1 - Funding Information:
This work was supported by the french Inserm (JV and BD).
PY - 2010/12
Y1 - 2010/12
N2 - Anticonvulsant properties of α-asarone were studied in mice at three doses with different toxicity. The 100 mg/kg dose decreased both treadmill performance and locomotor activity, caused hypothermia, and potentiated pentobarbital-induced sleep. The last two effects and no toxicity were observed at 60 and 22 mg/kg, respectively. In chemical (pentylenetetrazole, picrotoxin, N-methyl- d-aspartate, pilocarpine) and electrical (maximal electroshock) seizure tests, neither seizures nor death were prevented by 60 mg/kg α-asarone which, however, exhibited protective-like effects (delay in the onset of clonic and/or tonic seizures and/or in the death of mice). Magnesium deficiency-dependent audiogenic seizures responded to non-toxic doses of α-asarone (60 mg/kg and less): 22 mg/kg protecting 50% of tested animals. Because these seizures respond to both anti-seizure and antioxidant compounds, antioxidant properties of α-asarone were studied, indicating 5. Units of superoxide dismutase-like activity per mg α-asarone. Treatment of mice by α-asarone (daily dose of 100 mg/kg during 7 days) induced brain antioxidant enzymes (superoxide dismutase, glutathione peroxidase and reductase) in striatum and hippocampus and to a lesser extent in cortex. In view of recent findings about deleterious roles of chronic inflammatory/oxidant stresses in human epilepsy outcome, antioxidant and inductive properties of α-asarone are proposed to be coherent bases for traditional clinical efficacy.
AB - Anticonvulsant properties of α-asarone were studied in mice at three doses with different toxicity. The 100 mg/kg dose decreased both treadmill performance and locomotor activity, caused hypothermia, and potentiated pentobarbital-induced sleep. The last two effects and no toxicity were observed at 60 and 22 mg/kg, respectively. In chemical (pentylenetetrazole, picrotoxin, N-methyl- d-aspartate, pilocarpine) and electrical (maximal electroshock) seizure tests, neither seizures nor death were prevented by 60 mg/kg α-asarone which, however, exhibited protective-like effects (delay in the onset of clonic and/or tonic seizures and/or in the death of mice). Magnesium deficiency-dependent audiogenic seizures responded to non-toxic doses of α-asarone (60 mg/kg and less): 22 mg/kg protecting 50% of tested animals. Because these seizures respond to both anti-seizure and antioxidant compounds, antioxidant properties of α-asarone were studied, indicating 5. Units of superoxide dismutase-like activity per mg α-asarone. Treatment of mice by α-asarone (daily dose of 100 mg/kg during 7 days) induced brain antioxidant enzymes (superoxide dismutase, glutathione peroxidase and reductase) in striatum and hippocampus and to a lesser extent in cortex. In view of recent findings about deleterious roles of chronic inflammatory/oxidant stresses in human epilepsy outcome, antioxidant and inductive properties of α-asarone are proposed to be coherent bases for traditional clinical efficacy.
KW - Antioxidant
KW - Audiogenic test
KW - Electroshock
KW - Epilepsy
KW - Magnesium deficiency
KW - N-methyl-d-aspartate
KW - Neuroprotection
KW - Pentylenetetrazole
KW - Picrotoxin
KW - Pilocarpine
KW - Seizure
KW - α-Asarone
UR - http://www.scopus.com/inward/record.url?scp=78149471472&partnerID=8YFLogxK
U2 - 10.1016/j.neures.2010.08.011
DO - 10.1016/j.neures.2010.08.011
M3 - Artículo
SN - 0168-0102
VL - 68
SP - 337
EP - 344
JO - Neuroscience Research
JF - Neuroscience Research
IS - 4
ER -