TY - JOUR
T1 - Active tuberculosis patients have high levels of IgA anti-alphacrystallin and isocitrate lyase proteins
AU - Talavera-Paulín, M.
AU - García-Morales, L.
AU - Ruíz-Sánchez, B. P.
AU - Caamal-Ley, A. D.
AU - Hernández-Solis, A.
AU - Ramírez-Casanova, E.
AU - Cicero-Sabido, R.
AU - Espitia, C.
AU - Helguera-Repetto, C.
AU - Gonzálezy-Merchand, J. A.
AU - Flores-Mejía, R.
AU - Estrada-Parra, S.
AU - Estrada-García, I.
AU - Chacón-Salinas, R.
AU - Wong-Baeza, I.
AU - Serafín-López, J.
N1 - Publisher Copyright:
© 2016 The Union.
PY - 2016/12/1
Y1 - 2016/12/1
N2 - SETTING: Mexico City, Mexico. OBJECTIVE : To identify proteins synthetised by Mycobacterium tuberculosis in hypoxic culture, which resemble more closely a granuloma environment than aerobic culture, and to determine if they are recognised by antibodies from patients with active pulmonary tuberculosis (PTB). DESIGN: Soluble extracts from M. tuberculosis H37Rv cultured under aerobic or hypoxic conditions were analysed using two-dimensional polyacrylamide gel electrophoresis, and proteins over-expressed under hypoxia were identified by mass spectrometry. The presence of immunoglobulin (Ig) G, IgA and IgM antibodies against these proteins was determined in the serum of 42 patients with active PTB and 42 healthy controls. RESULT S : We selected three M. tuberculosis H37Rv proteins (alpha-crystallin protein [Acr, Rv2031c], universal stress protein Rv2623 and isocitrate lyase [ICL, RV0467]) that were over-expressed under hypoxia. Titres of anti-Acr and anti-ICL IgA antibodies were higher in patients than in healthy controls, with an area under the receiver operating characteristic curve of 0.71 for anti-ICL IgA antibodies. CONCLUS ION: ICL could be used in combination with other M. tuberculosis antigens to improve the sensitivity and specificity of current serological TB diagnostic methods.
AB - SETTING: Mexico City, Mexico. OBJECTIVE : To identify proteins synthetised by Mycobacterium tuberculosis in hypoxic culture, which resemble more closely a granuloma environment than aerobic culture, and to determine if they are recognised by antibodies from patients with active pulmonary tuberculosis (PTB). DESIGN: Soluble extracts from M. tuberculosis H37Rv cultured under aerobic or hypoxic conditions were analysed using two-dimensional polyacrylamide gel electrophoresis, and proteins over-expressed under hypoxia were identified by mass spectrometry. The presence of immunoglobulin (Ig) G, IgA and IgM antibodies against these proteins was determined in the serum of 42 patients with active PTB and 42 healthy controls. RESULT S : We selected three M. tuberculosis H37Rv proteins (alpha-crystallin protein [Acr, Rv2031c], universal stress protein Rv2623 and isocitrate lyase [ICL, RV0467]) that were over-expressed under hypoxia. Titres of anti-Acr and anti-ICL IgA antibodies were higher in patients than in healthy controls, with an area under the receiver operating characteristic curve of 0.71 for anti-ICL IgA antibodies. CONCLUS ION: ICL could be used in combination with other M. tuberculosis antigens to improve the sensitivity and specificity of current serological TB diagnostic methods.
KW - 2D-PAGE/MS
KW - Hypoxia
KW - Serological diagnostic methods
UR - http://www.scopus.com/inward/record.url?scp=84998996020&partnerID=8YFLogxK
U2 - 10.5588/ijtld.16.0250
DO - 10.5588/ijtld.16.0250
M3 - Artículo
C2 - 28000588
SN - 1027-3719
VL - 20
SP - 1681
EP - 1688
JO - International Journal of Tuberculosis and Lung Disease
JF - International Journal of Tuberculosis and Lung Disease
IS - 12
ER -