TY - JOUR
T1 - Actinobacterial diversity from marine sediments collected in Mexico
AU - Maldonado, Luis A.
AU - Fragoso-Yáñez, Dulce
AU - Pérez-García, Adriana
AU - Rosellón-Druker, Judith
AU - Quintana, Erika T.
N1 - Funding Information:
Acknowledgements This work is sponsored by grants 612 (ICMyL-UNAM; Mexican aquatic environments) and CO1-46894 (Consejo Nacional de Ciencia y Tecnología, Mexico - CONACyT-; Gulf of California) to LAMM. DFY received a scholarship from project CO1-46894 to study some of the isolates included in this work.
PY - 2009
Y1 - 2009
N2 - Seventeen different media known to support the growth and isolation of members of the class Actinobacteria were evaluated as selective isolation media for the recovery of this microbial group from marine sediments samples collected in the Gulf of California and the Gulf of Mexico. A general selective isolation procedure was employed for six sediments and nearly 300 actinomycetes were recovered from the selective isolation plates. Full 16S rRNA gene sequencing revealed that the isolates belonged to several actinobacterial taxa, notably to the genera Actinomadura, Dietzia, Gordonia, Micromonospora, Nonomuraea, Rhodococcus, Saccharomonospora, Saccharopolyspora, Salinispora, Streptomyces, "Solwaraspora" and Verrucosispora. Previous works on marine sediments have been restricted to the isolation of members of the genera Micromonospora, Rhodococcus and Streptomyces. This study provides further evidence that Actinobacteria present in marine habitats are not restricted to the Micromonospora-Rhodococcus- Streptomyces grouping. Indeed, this first systematic study shows the extent of actinobacterial diversity that can be found in marine sediments collected in Mexico and probably, worldwide.
AB - Seventeen different media known to support the growth and isolation of members of the class Actinobacteria were evaluated as selective isolation media for the recovery of this microbial group from marine sediments samples collected in the Gulf of California and the Gulf of Mexico. A general selective isolation procedure was employed for six sediments and nearly 300 actinomycetes were recovered from the selective isolation plates. Full 16S rRNA gene sequencing revealed that the isolates belonged to several actinobacterial taxa, notably to the genera Actinomadura, Dietzia, Gordonia, Micromonospora, Nonomuraea, Rhodococcus, Saccharomonospora, Saccharopolyspora, Salinispora, Streptomyces, "Solwaraspora" and Verrucosispora. Previous works on marine sediments have been restricted to the isolation of members of the genera Micromonospora, Rhodococcus and Streptomyces. This study provides further evidence that Actinobacteria present in marine habitats are not restricted to the Micromonospora-Rhodococcus- Streptomyces grouping. Indeed, this first systematic study shows the extent of actinobacterial diversity that can be found in marine sediments collected in Mexico and probably, worldwide.
KW - 16S rRNA gene sequencing
KW - Actinobacteria
KW - Marine sediments
KW - Selective isolation
UR - http://www.scopus.com/inward/record.url?scp=58949097527&partnerID=8YFLogxK
U2 - 10.1007/s10482-008-9294-3
DO - 10.1007/s10482-008-9294-3
M3 - Artículo
SN - 0003-6072
VL - 95
SP - 111
EP - 120
JO - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
JF - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
IS - 2
ER -